Yeast chitin synthases 1 and 2 consist of a non-homologous and dispensable N-terminal region and of a homologous moiety essential for function

Molecular and General Genetics

Volumn 252, Issue 4, 1996, Pages 420-428

  Ford, Richard A ^a   Shaw, J Andrew ^a,b   Cabib, Enrico ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b BIO RAD LABORATORIES   (United States)

Author keywords

Chitin synthases; Saccharomyces cerevisiae; Septum

Indexed keywords

CHITIN SYNTHASE; FUNGAL PROTEIN; MUTANT PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DELETION MUTANT; ENZYME ACTIVITY; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION;

SACCHAROMYCES CEREVISIAE;

EID: 0029852431     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02173007     Document Type: Article

References (33)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Greene Publishing Associates and Wiley-Interscience, New York
2. Boeke JD, Trueheart J, Natsoulis G. Fink GR (1987) 5-fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol 154 : 164-175
3. Bulawa CE (1992) CSD2, CSD3 and CHD4, genes required for chitin synthesis in Sacchromyces cererisiae. The CSD2 gene is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laeris. Mol Cell Biol 12 : 1764-1776
4. Bulawa CE, Osmond BC (1990) Chitin synthase I and chitin synthuse II are not required for chitin synthesis in vivo in Saccharomyces cereisiae. Proc Natl Acad Sci USA 87 : 7424-7428
5. Bulawa CE, Slater M, Cabib E, Au-Young J, Sburlati A, Adair WL, Robbins PW (1986) The S. cererisiae structural gene for chitin synthase is not required for chitin synthesis in vivo, Cell 46 : 213-225
6. Cabib E (1987) The synthesis and degradation of chitin, Adv Enzymol 59 : 59-101
7. Cabib E (1994) Nomenclature of genes related to chitin synthesis. Yeast Newsl 43 : 58
8. Cabib E, Farkas V (1971) The control of morphogenesis: an enzymatic mechanism for the initiation of septum formation in yeast. Proc Natl Acad Sci USA 68 : 2052-2056
9. Cabib E, Sburlati A, Bowers B, Silverman SJ (1989) Chitin synthase I, an auxiliary enzyme for chitin synthesis in Saccharomyces cererisiae. J Cell Biol 108 : 1665-1672
10. Cabib E, Silverman SJ, Shaw JA (1992) Chitinase and chitin synthase 1: counterbalancing activities in cell separation of Succharomyces cererisiae. J Gen Microbiol 138 : 97-102
11. Choi W-J, Cabib E (1994) The use of divalent cations and pH for the determination of specific yeast chitin synthetases. Anal Biochem 219 : 368-372
12. Choi W-J, Sburlati A, Cabib E (1994a) Chitin synthase 3 from yeast has zymogenic properties that depend on both the CAL1 and the CAL2 genes. Proc Natl Acad Sci USA 91 : 4727-4730
13. Choi W-J. Santos B, Durán A, Cabib E (1994b) Are yeast chitin synthases regulated at the transcriptional or the posttranslational level? Mol Cell Biol 14 : 7685-7694
14. Deng WP, Nickoloff JA (1992) Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal Biochem 200 : 81-88
15. Feng DF, Doolittle RF (1987) Progressive sequence alignment as u prerequisite to correct phylogenetic trees. J Mol Evol 25 : 351-360
16. Ito H, Fukuda Y, Muruta K. Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153 : 163-168
17. Kang MS, Au-Young J, Cabib E (1985) Modification of yeast plasma membrane density by concanavalin A attachment. Application to study of chitin synthetase distribution. J Biol Chem 260 : 12680-12684
18. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157 : 105-132
19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193 : 265-275
20. Molano J, Bowers B, Cabib E (1980) Distribution of chitin in the yeast cell wall. An ultrastructural and chemical study. J Cell Biol 85 : 199-212
21. Naguhashi S, Sudoh M, Ono N, Sawada R, Yamaguchi E, Uchida Y, Mio T, Takagi M, Arisawa M, Yamada-Okabe H (1995) Characterization of chitin synlhase 2 of Saceharomyces cererisiae. J Biol Chem 270 : 13961-13967
22. Orlean P (1987) Two chitin synthases in Saccharomyces cererisiae. J Biol Chem 262 : 5732-5739
23. Rammer M, Briza P, Ellinger A, Schuster T, Stucka R, Feldmann H, Breitenbach M (1992) DIT101 (CSD2, CALI). a cell-cycle regulated yeast gene required for synthesis of chitin in cell walls and chitosan in spore walls. Yeasts 8 : 1089-1099
24. Roncero C, Valdivieso MH, Ribas JC, Durán A (1988) Isolation and characterization of Sacecharomyces cererisiae mutants resistant to Calcofluor white. J Bacteriol 170 : 1950-1954
25. Ruiz-Herrera J (1992) Fungal cell wall: structure, synthesis, and assembly. CRC Press, Boca Raton
26. Sburlati A, Cabib E (1986) Chitin synthetase 2, a presumptive participant in septum formation in Sacchamyces cererisiae. J Biol Chem 261 : 15147-15152
27. Shaw JA, Mol PC, Bowers B, Silverman SJ, Valdivieso MH, Durán A, Cabib E (1991) The function of chitin synthases 2 and 3 in the Saccharomyces cererisiae cell cycle. J Cell Biol 114 : 111-123
28. Sikorski RS, Heiter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cererisiae. Genetics 122 : 19-27
29. Silverman SJ (1989) Similar and different domains of chitin synthases 1 and 2 of S. cererisiae: two isozymes with distinct functions. Yeast 5 : 459-467
30. Silverman SJ, Sburlati A, Slaster ML, Cabib E (1988) Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cererisiae. Proc Natl Acad Sci USA 85 : 4735-4739
31. Smith TF, Waterman MS (1981) Comparison of bio-sequences. Adv Appl Math 2 : 482-489
32. Sudoh M, Nagahashi S, Doi M, Ohta A, Takagi M, Arisawa M (1993) Cloning of the chitin synthase 3 gene from Candida albi-Cans and its expression during yeast-hyphal transition. Mol Gen Genet 241 : 351-358
33. Valdivieso MH, Mol PC, Shaw JA, Cabib E, Duran A (1991) CALI, a gene required for activity of chitin synthase 3 in Saccharomyces cererisiae. J Cell Biol 114 : 101-109