Detection of human T lymphotrophic virus type I (HTLV-I) DNA and mRNA in individual cells by polymerase chain reaction (PCR) in situ hybridization (ISH) and reverse transcription (RT)-PCR ISH

Hematological Oncology

Volumn 14, Issue 2, 1996, Pages 91-100

  Ohshima, Koichi ^a   Hashimoto, Kenichiro ^b   Izumo, Shuji ^c   Suzumiya, Junji ^a   Kikuchi, Masahiro ^a  

^a FUKUOKA UNIVERSITY   (Japan)

^b KAGOSHIMA UNIVERSITY   (Japan)

^c KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MESSENGER RNA; PROVIRUS DEOXYRIBONUCLEIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN T CELL LEUKEMIA VIRUS 1; MONONUCLEAR CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; T CELL LEUKEMIA;

DNA, VIRAL; HL-60 CELLS; HUMAN T-LYMPHOTROPIC VIRUS 1; HUMANS; IN SITU HYBRIDIZATION; LEUKEMIA, T-CELL; POLYMERASE CHAIN REACTION; PROVIRUSES; RNA, MESSENGER; RNA, VIRAL;

EID: 0029851748     PISSN: 02780232     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1069(199606)14:2<91::AID-HON574>3.0.CO;2-G     Document Type: Article

References (16)

1. Hinuma, T., Gotoh, Y., Sugamura, K., et al. A retrovirus associated with human adult T-cell leukemia: in vitro activation. Gann 1982, 73, 341-344.
2. Takatsuki, K. Overview of adult T-cell leukemia/lymphoma. Jpn. Soc. Res. 1985, 25, 97-103.
3. Yoshida, M., Seiki, M., Yamaguchi, K., Takatsuki, K. Monoclonal integration of human T-cell leukemia provirus in all primary tumours of adult T-cell leukemia suggests a causative role of human T-cell leukemia virus in the disease. Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 2534-2537.
4. Seiki, M., Inoue, J., Takeda, T., Yoshida, M. Direct evidence that p40x of human T-cell leukemia virus type I is a trans-acting transcriptional activator. EMBO J. 1986, 5, 561-565.
5. Kinoshita, T., Shimoyama, M., Tobinai, K., et al. Detection of mRNA for the tax1/rex1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction. Proc. Natl. Acad. Sci. U.S.A. 1989, 86, 5620-5624.
6. Haase, A. T., Retzel, E. F., Staskus, K. A. Amplification and detection of lentiviral DNA inside cells. Proc. Natl. Acad. Sci. U.S.A. 1990, 87, 4971-4975.
7. Nuovo, G. J., MacConnell, P., Forde, A., Delvenne, P. Detection of human papillomavirus DNA in formalin fixed tissues by in situ hybridization after amplification by PCR. Am. J Pathol. 1991, 139, 847-854.
8. Nuovo, G. J., Gallery, F., MacConnell, P., Becker, J., Bloch, W. An improved technique for the in situ detection of DNA after polymerase chain reaction amplification. Am. J. Pathol. 1991, 139, 1239-1244.
9. Patterson, B. K., Till, M., Otto, P., et al. Detection of HIV-1 DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry. Science 1993, 260, 976-979.
10. Ohshima, K., Kikuchi, M., Masuda, Y., et al. Defective provirus form of human T-cell leukemia virus type I in adult T-cell leukemia/lymphoma: clinicopathologic features. Cancer Res. 1991, 51, 4639-4642.
11. Seiki, M., Hattori, S., Hirayama, Y., Yoshida, M. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc. Natl. Acad. Sci. U.S.A. 1983, 80, 3618-3622.
12. Kashio, N., Izumo, S., Ijichi, S., et al. Detection of HTLV-1 provirus by in situ polymerase chain reaction in mononuclear cells infiltrating spinal cord lesions from HAM/TSP patients. J. Neurovirol. 1995, in press.
13. Chang, K. L., Chen, Y. Y., Shibata, D., Weiss, L. M. Description of an in situ hybridization methodology for detection of Epstein-Barr virus RNA in paraffin-embedded tissues, with a survey of normal and neoplastic tissues. Diagn. Mol. Pathol. 1992, 1, 246-255.
14. Nuovo, G. J. In situ PCR: protocols and application. Manual Supplement by Cold Spring Harbor Laboratory 1995, 4, 151-167.
15. Fujii, M., Sassone-Corsi, P., Verma, I. M. c-fos promoter trans-activation by the tax1 protein of human T-cell leukemia virus type I. Proc. Natl. Acad. Sci. U.S.A. 1988, 85, 8526-8530.
16. Cross, S. L., Feinberg, M. B., Wolf, J. B., Holbrook, J. J., Wong-Staal, F., Leonard, W. J. Regulation of the human interleukin-2 receptor alpha chain promoter: activation of a nonfunctional promoter by the transactivator gene of HTLV-1. Cell 1987, 49, 47-56.