Attractant signaling by an aspartate chemoreceptor dimer with a single cytoplasmic domain

Science

Volumn 274, Issue 5286, 1996, Pages 425-426

  Gardina, Paul J ^a   Manson, Michael D ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; DIMER; MEMBRANE RECEPTOR;

ARTICLE; CHEMORECEPTOR; CHEMOTAXIS; CYTOPLASM; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ASPARTIC ACID; BACTERIAL PROTEINS; BINDING SITES; CHEMORECEPTORS; CHEMOTAXIS; CYTOPLASM; DIMERIZATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC COMPLEMENTATION TEST; MEMBRANE PROTEINS; MUTATION; PLASMIDS; PROTEIN CONFORMATION; PROTEIN KINASES; RECEPTORS, CELL SURFACE; SIGNAL TRANSDUCTION;

ENTERIC BACTERIA; ESCHERICHIA COLI;

EID: 0029851704     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5286.425     Document Type: Article

References (27)

1. Well-characterized receptors of this type include those for epidermal [Y. Yarden and J. Schlessinger, Biochemistry 26, 1443 (1987)] and human [B. C. Cunningham et al., Science 254, 821 (1991)) growth factors and insulin [S. R. Hubbard, L. Wei, L. Ellis, W. A. Hendrickson, Nature 372, 746 (1994)]. For a general review, see C.-H. Heldin, Cell 80, 213 (1995).
2. Well-characterized receptors of this type include those for epidermal [Y. Yarden and J. Schlessinger, Biochemistry 26, 1443 (1987)] and human [B. C. Cunningham et al., Science 254, 821 (1991)) growth factors and insulin [S. R. Hubbard, L. Wei, L. Ellis, W. A. Hendrickson, Nature 372, 746 (1994)]. For a general review, see C.-H. Heldin, Cell 80, 213 (1995).
3. Well-characterized receptors of this type include those for epidermal [Y. Yarden and J. Schlessinger, Biochemistry 26, 1443 (1987)] and human [B. C. Cunningham et al., Science 254, 821 (1991)) growth factors and insulin [S. R. Hubbard, L. Wei, L. Ellis, W. A. Hendrickson, Nature 372, 746 (1994)]. For a general review, see C.-H. Heldin, Cell 80, 213 (1995).
4. Well-characterized receptors of this type include those for epidermal [Y. Yarden and J. Schlessinger, Biochemistry 26, 1443 (1987)] and human [B. C. Cunningham et al., Science 254, 821 (1991)) growth factors and insulin [S. R. Hubbard, L. Wei, L. Ellis, W. A. Hendrickson, Nature 372, 746 (1994)]. For a general review, see C.-H. Heldin, Cell 80, 213 (1995).
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15. Strain VB12 is a derivative of strain RP437 [J. S. Parkinson, ibid. 135, 45 (1978)] containing chromosomal transducer deletions (Δtsr-7021 Δtar-tap5201). Strains lacking both Tar and Tsr (the serine receptor) are generally nonchemotactic. The experiments were repeated with the recA56 strain, KO607 [K. Oosawa, N. Mutoh, M. I. Simon, ibid. 170, 1326 (1988)], which produced the same complementation patterns.
16. Strain VB12 is a derivative of strain RP437 [J. S. Parkinson, ibid. 135, 45 (1978)] containing chromosomal transducer deletions (Δtsr-7021 Δtar-tap5201). Strains lacking both Tar and Tsr (the serine receptor) are generally nonchemotactic. The experiments were repeated with the recA56 strain, KO607 [K. Oosawa, N. Mutoh, M. I. Simon, ibid. 170, 1326 (1988)], which produced the same complementation patterns.
17. Colonies that respond to aspartate form characteristic expanding rings in soft (0.325%) agar containing 0.1 mM aspartate (9). Chemotactic competence is judged by expansion rate and ring sharpness.
18. P. Gardina and M. Manson, unpublished results.
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21. M. A. Danielson, H. P. Biemann, D. E. Koshland Jr., J. J. Falke, Biochemistry 33, 6100 (1994); G. F. Lee, M. R. Lebert, A. A. Lilly, G. L. Hazelbauer, Proc. Natl. Acad. Sci. U.S.A. 92, 3391 (1994). Signaling asymmetry with respect to the binding site was reported for a Tar-EnvZ fusion protein [Y. Yang, H. Park, M. Inouye, J. Mol. Biol. 232, 493 (1993)].
22. A deletion derivative of this plasmid, lacking the sequence encoding the COOH-terminal 60% of the cytoplasmic domain, was also constructed. Both plasmids mediated identical responses, indicating that read-through of the UAA codon was insignificant.
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27. We thank R. Bourret for plasmid pRBB16 and the antibody to Tar. I. Kawagishi, K. Oosawa, and D. E. Koshland Jr. provided helpful suggestions. Supported by NIH grant GM39736.