Human intestinal epithelial cells swell and demonstrate actin rearrangement in response to the metalloprotease toxin of Bacteroides fragilis

Infection and Immunity

Volumn 64, Issue 12, 1996, Pages 5022-5028

  Koshy, Sherin ^a   Montrose, Marshall H ^a   Sears, Cynthia L ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN; F ACTIN; METALLOPROTEINASE;

ACTIN FILAMENT; ARTICLE; BACTEROIDES FRAGILIS; CELL VOLUME; CONTROLLED STUDY; CYTOSKELETON; DIARRHEA; HUMAN; HUMAN CELL; INTESTINE CELL; INTESTINE EPITHELIUM; PRIORITY JOURNAL; PROTEIN DEGRADATION; TIGHT JUNCTION;

EID: 0029849199     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.12.5022-5028.1996     Document Type: Article

References (45)

1. Collins. J. E., M. E. Bergeland, L. L. Myers, and D. S. Shoop. 1989. Exfoliating colitis associated with enleroloxigenic Bactfroider fragilis in a piglet. J. Vol. Diagn. Invest, 1:349-351.
2. Cossart, P., P. Briquet, S. Normark, and R. Rappuoli. 1996. Cellulur microbiology emerging. Science 271:315-316.
3. Dabiri, G. A., J. M. Sanger, D. A. Portnoy, and F. S. Southwick. 1990. Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly. Proc. Natl. Acad, Sci. USA 87:6068-6072.
4. DaneIll, G., A. Fabhri, and C. Florentinl. 1996. Bacteroides fragilis cnterotoxin induces cytoskeletal changes und surface Webbing in HT-29 cells. Infect. Immun, M:113-119.
5. Duimstra, J. R., L, L. Myers, J. E. Collins, D. A. Benneld, D. S. Shoop, and W. C. Bradbury. 1991. Enterovirulence of enterutoxigenic Bacteroides fragilis in gnotobiotic pigs. Vet. Pathol. 28:514-518.
6. Florentin!, C., G. Donelli, P. Matarrese, A. Fabbri, S. Paradisl, and P. Hoquet. 1995, Escherichia coli cytotoxic necrotizing factor 1: evidence for Induction of actin assembly by constitutive activation of the p21 Rho GTPase. Infect. Immun. 63:3936-3944.
7. Foskett, J. K., and K. K. Spring. 1985. Involvement of calcium and cytoskcleton in gallbladder epithelial cell volume regulation, Am. J. Physiol. 248: C27-C36.
8. Hallows. K. R., F.-Y. Law, C. H, Packman, and P. A. Knauf, l996. Changes in cytoskcletal actin content, F-actin distribution and surface morphology during Hl.-60 cell volume regulation, J. Cell. Physiol. 167:60-71,
9. Hecht, G., A. Knutsnurls, C, Pothoulakis, J. T. LaMont, and J. L. Madara. 1992. Chostridium difficile toxin B disrupts the barrier function of T84 monolayers, Gastroenterology 102:416-423
10. Hecht, G., C. Pothoulakls, J. T. LaMont, and J. L. Madara. 1988. Closmdium difficile toxin A perturbs eytoskeletal structure and tight junction permeability of cultured human intestinal epithelial monolayers. J. Clin. Invest. 82: 1510-1524.
11. Herman, I. M. 1993. Actin isoforms. Curr. Opin. Cell. Biol. 5:48-55.
12. Hewlett, K. L., M. C. Gray, I. E, Ehrmann, N. J. Maloney, A. S. Otero, L. Gray, M, Allietta, G. Szabo, A. A. Weiss, and E. M. Harry. 1993. Characterization of adenylale cyclase toxin from a mutant of Bordetella pertussis defective in the activator gene, cyaC. J. Biol. Chem. 268:7842-7848.
13. Huet, C., C. Sahuquillo-Merino, E, Coudrier, and D. Louvard. 1987 Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT29) provide new models for cell polarity and terminal differentiation. J. Cell Biol. 105:345-357.
14. Just, L., M. Wilm, J. Selzer, G. Rex, C. von Eichel-Streiber, M. Mann, and K. Aktories. 1995. The enlerotoxin from Clasiridium difficile (ToxA) monoglucosylates the Rho proteins, J. Biol. Chem. 270:13932-13936.
15. Knutton, S., T. Balwin, P. H. Williams, and A. S. McNelsh. 1989, Actin aecumulalion at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropalhogenic and entcrohemorrhagic Kseherlclila coli. Infect. Immun. 57:1290-1298.
16. Lang, F., G. dusch, H, Volkl, and D. Haussinger. 1995. Cell volume: a second message in regulation of cellular function. News Physiol, Sci. 10:1822,
17. Madara, J. L., J, Stafford, D. Barenherg, and S. Carlson. 1988. Functional coupling of tight junctions and microfilaments in T84 monolayers. Am. J. Physiol. 254:G416-G423.
18. + cotransport by eAMP in intestinal epithelial monoliiyers. J. Clin. Invest. 90:1608-1613.
19. + K 2CI cotransporl and CI secretion evoked by heatstable enleroloxin is microfilament dependent in T84 cells. Am. J. Physiol. 265:0370-0378.
20. Matthews, J. B., J. A. Smith, K. J. Tally, C. S. Awtray, H. Nguyen, J. Rich, and J. L. Madara. 1994. Na ' K R 2CI cotransport in intestinal epithelial cells: influence of chloride efflux and F actin on regulation of cotransporter activity and humetanide binding. J. Biol. Chem. 269:15703-15709.
21. McClanc. B. A. 1994, Clostridium perfringens acts by producing small molecule permeability alterations in plasma membranes, Toxicology 87:43-ft7,
22. Mellor, I. R., D. H. Thumas, and M. S. P. Sansom. 1988. Properties of ion channels formed by Staphylococeusaureus 8-toxin. Biochim. Biophys. Acta 942:280-294.
23. Moncricf, J. S., R. Obiso, Jr., L. A. Rarrasn, J. J, Kling, R. L. Wright, R. L. Van Tasscll, D. M. Lyerly, and T. D. Wilkins. 1995. The enleroloxin of Bacteroides fragilis is a metalloprotease. Infect. Immun. 63:175-181.
24. Mounler, J., A. Kyter, M. Coquls-Rondon, and P. J. Sarsonetli, 1990. Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enleroeyte-like cell line Caco-2. Infect. Immun. 58:1048-1058.
25. Mundy, L M., and C. L. Seurs. Toxin production by Bacteroides frailis: detection in extraintestinal clinical isolates, Clin. Infect. Dis., in press.
26. Myers, L. L., B. D. Flrehammer, D. S. Shoop, and M. M. Border. 1984. Bacteroides fragilis: a possible cause of acute diarrhcal disease in newborn lambs. Infect. Immun. 44:241-244.
27. Myers, L. L., D. S. Shoop, and J. E. Colllns. 1990. Rabbit model to evaluate enterovirulence of Bacteroides fragllis. J. Clin. Microbiol. 28:1658-1660.
28. Myers, L. L., D. S. Shoop, J. E. Colllns, and W. C. Bradbury. 1989. Diarrheal disease caused by enterotoxigenic Bacteroides fragilis in infant rabbits. J. Clin. Microbiol. 27:2025-2030.
29. Myers, L. L., D. S. Shoop, B. D. Flrehammer, and M. M. Border. 1985. Association of enterotoxigenic Bacleroides fragilis with diarrheal disease in calves. J. Infect. Dis. 152:1344-1348.
30. Oblso, R. J., D. M. Lyerly, R. L. Van Tassell, and T. D. Wlkins. 1995. Proteolytic activity of the Bacteroides fragilis enterotoxin causes fluid secretion and intestinal damage in vivo. Infect. Immun. 63:3820-3826.
31. Pothoulakis, C., J. T. LaMont, R. Eglow, N. Gao, J. B. Rubins, T. C. Theoharldes, and B. F. Dickey. 1991. Characterization of rabbit ileal receptors for Clostridium difficile toxin A: evidence for a receptor-coupled G protein. J. Clin. Invest. 88:119-125.
32. Rosenshine, I., and B. B. Flnlay. 1993. Exploitation of host signal transduction pathways and cytoskclctal functions by invasive bacteria. Bioessays 15: 17-24.
33. Rowe, W. A., D. L. Blaekmon, and M. H. Montrose. 1993. Propionate activates multiple ion transport mechanisms in the HT29-18-C1 human colon cell line. Am. J. Physiol. 265:0564-0571.
34. Sack, R. B., M. J. Albert, K. Alam, P. K. B. Neogl, and M. S. Akbar. 1994. Isolation of enterotoxigenic Bacleroides fragilis from Bangladeshi children with diarrhea: a controlled study. J. Clin. Microbiol. 32:960-963.
35. Sack, R. B., L. L. Myers, J, A, Hill, D. S. Shoop, W. C. Bradbury, R. Held, and M. Santosham. 1992. Enterotoxigenic Bacleroides fragilis: epidemiologic studies of its role as a human diarrhoeal pathogen. J. Diarrhoeal Dis. Res. 10:4-9.
36. Sanger, J. M., J. W. Sanger, and F. S. Southwick. 1992. Host cell actin assembly is necessary and likely to provide the propulsive force for intracellular movement of Lisleria monocytogenes. Infect. Immun. 60:3609-3619.
37. San Joaquln, V. H., J. C. Griffis, C. Lee, and C. L. Sears. 1995. Association of Bacteroides fragilis with childhood diarrhea, Scand. J. Infect, Dis. 27:211-215.
38. Sears, C. L., and J. B. Kaper. 1996. Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion. Microhiol. Rev. 60:167-215.
39. Sears, C. L., L. L Myers, A. Lazenby, and R. L. Van Tassell. 1995. Enterotoxigenic Bacteroides fragilis: a summary. Clin, Infect. Dis. 20(Suppl,):S142-S148,
40. Shapiro, M., J. Matthews, G, Hecht, C. Delp, and J. L. Madara. 1991, Stabilization of F-actin prevents cAMP-elicitcd Cl- secretion in T84 cells, J. Clin. Invest. 87:1903-1909.
41. Sujlmoto, N., M. Takagi, K. Ozutiumi, S, Harada, and M. Matsuda. 1988. Enterotoxin of Clostridium perfringens type A forms ion-permeable channels in a lipid bilayer membrane. Biochem. Biophys, Res. Commun. 156: 551-556.
42. Van Tassell, R. L., D, M. Lyerly, and T. D. Wllklns. 1992. Purification and characterization of an enterotoxin from Bacteroides fragilis. Infect. Immun. 60:1343-1350.
43. Vassclon, T., J. Mounter, R. Helllo, and P. S. Sansonettl. 1992. Movement along actin filaments of the perijunctional area and de novo polymerization of cellular actin are required for Shigella ftexneri colonization of epithelial Caco-2 cell monolayers. Infect, Immun. 60:1031-1040.
44. Watson, A. J. M., S. Levine, M. Donowltz, and M. H. Montroie. 1992. Serum regulates Na-H?? exchange in Caco-2 cells by a mechanism which is dependent on F-actin. J. Biol. Chem. 267:956-962.
45. Welkel, C. L., F. D. Grieco, J. Reuben, L. L. Myers, and R. B. Sack. 1992. Human colonie epithelial cells, HT29/C, treated with crude Bacteroides fragilis entcroloxin dramatically alter their morphology. Infect. Immun, 60: 321-327.