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Volumn 273, Issue 5278, 1996, Pages 1112-1114

Selective activation of calcium permeability by aspartate in Purkinje cells

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINO 5 PHOSPHONOVALERIC ACID; 6 CYANO 7 NITRO 2,3 QUINOXALINEDIONE; ALPHA AMINO 3 HYDROXY 5 METHYL 4 ISOXAZOLEPROPIONIC ACID; ASPARTIC ACID; CALCIUM ION; EXCITATORY AMINO ACID; GLUTAMIC ACID; HOMOCYSTEIC ACID; KAINIC ACID; MAGNESIUM ION; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; N METHYL DEXTRO ASPARTIC ACID RECEPTOR BLOCKING AGENT;

EID: 0029847062     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5278.1112     Document Type: Article
Times cited : (37)

References (31)
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    • 50 = 233 μM). In addition, although the inactivating component often diminished during prolonged recordings (> 30 min), there was little change in the noninactivating component (17 of 29 prolonged recordings). The presence of two components may account for the insensitivity of Asp currents to AFV [E. Audinat, T. Knöpfel, B. H. Gähwiler, J. Physiol. (London) 430, 297 (1990)]; that is, if the method of agonist application was slow, as is generally the case in slice preparations, the inactivating component is never fully expressed, leaving only the noninactivating component that we find to be less sensitive to NMDA receptor antagonists. The two components could be a consequence of several conducting states of a single molecule; alternatively, they could reflect the involvement of several different molecules.
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    • Audinat, E.1    Knöpfel, T.2    Gähwiler, B.H.3
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    • note
    • Coapplication of glycine (1 μM) increased the currents induced by Asp (300 μM) to 148 ± 13% of controls in wild-type Purkinje cells (n = 4). The currents induced by Asp (300 μM) + glycine (1 μM) were reduced by a noncompetitive NMDA receptor antagonist (5R,10S)-(+)-5-methyl-10,11 -dihydro-5H-dibenzo[a,d]cyctohepten-5,10-imine hydrogen maleate (MK-801) (5 μM) and by a noncompetitive non-NMDA receptor antagonist [1-(4-aminophenyl)-4-methyl-7,8-methyl-enedioxy-5H-2,3-benzodizepine HCl] (GYKI52466) (10 μM) to 14 ± 4% and 2.3 ± 1% of controls, respectively (n = 6).
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    • D. J. Perkel, S. Hestrin, P. Sah, R. A. Nicoll, Proc. R. Soc. London Ser. B 241, 116 (1990); A. Aijima, T. Hensh, R. T. Kado, M. Ito, Neurosci. Res. 12, 281 (1991).
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    • note
    • NR1 genotypes were determined with DMA samples from tail clips and analyzed by polymerase chain reaction (6).
  • 27
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    • 2+ was omitted from the external solution throughout the experiments. Tetrodotoxin (1 μM) and picrotoxin (100 μM) were included in the solution to block spontaneous electrical activity and glycine-γ-aminobutyric acid (GABA) channels. All drugs were dissolved in the recording solution. Drugs were applied by the Y-tube method [K. Murase et al., Brain Res. 525, 84 (1990)] controlled by a computer (6). A time constant of solution exchange surrounding a neuron was estimated to be about 15 to 20 ms, judging from a rise time of Gluinduced currents. Membrane potential was corrected for the liquid-junction potential.
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    • Murase, K.1
  • 28
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    • note
    • 2+ for 4 s at a series of membrane potentials close to the reversal potentials. Because complete solution exchange surrounding the neurons takes 15 to 20 ms (24), the reversal potentials measured by this method could underestimate the real value. Thus, for the determination of the reversal potentials, the current at 1 s after application of the test solution was measured.
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    • note
    • + ions.
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    • note
    • We thank S. C. Sun and L. M. Verselis for technical support and S. Hestrin for critical discussion. Supported, in part, by the International Human Frontier Science Program Organization (LT-663/93), National Institutes of Health Cancer Center Support CORE grant P30CA21765, and the American Lebanese Syrian Associated Charities.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.