Use of engineered thrombin cleavage site for determination of translational reading frames

BioTechniques

Volumn 21, Issue 5, 1996, Pages 822-824

  Zeng, Gang ^a   Larson, Timothy J ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; OLIGONUCLEOTIDE; THROMBIN;

ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; CELL LINE; ESCHERICHIA COLI; EXPRESSION VECTOR; FRAMESHIFT MUTATION; GENETIC ENGINEERING; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; RESTRICTION SITE; RNA TRANSLATION; STOP CODON;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029846994     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215bm15     Document Type: Article

References (4)

1. Carty, S.M., A. Colbeau, P.M. Vignais and T.J. Larson. 1994. Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus. FEMS Microbiol. Lett. 118:227-232.
2. Kasher, M.S., M. Wakulchick, J.A. Cook and M.C. Smith. 1993. One-step purification of recombinant human papillomavirus type 16 E7 oncoprotein and its binding to the retinoblastoma gene product. BioTechniques 14: 630-641.
3. Larson, T.J., S. Ye, D.L. Weissenborn, H.J. Hoffmann and H. Schweizer. 1987. Purification and characterization of the glp repressor of Escherichia coli K-12. J. Biol. Chem. 262:15869-15874.
4. Tabor, S. and C.C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.