EGF-receptor RNA metabolism in the nucleus of A431 cells

European Journal of Cell Biology

Volumn 71, Issue 1, 1996, Pages 45-52

  Sibon, Ody C M ^a,c   Wansink, Derick G ^b   Boonstra, Johannes ^a   Humbel, Bruno M ^a   Verkleij, Arie J ^a   Cremers, Fons F M ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

^c STONY BROOK UNIVERSITY   (United States)

Author keywords

EGF receptor; Electron microscopy; In situ hybridization; Nucleus; RNA; RNA metabolism

Indexed keywords

ALPHA AMANITIN; EPIDERMAL GROWTH FACTOR RECEPTOR;

ANIMAL CELL; ARTICLE; CANCER CELL CULTURE; CELL METABOLISM; CELL NUCLEUS; EPIDERMIS CELL; EXON; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; INTRON; MOUSE; NONHUMAN; NUCLEOLUS; PRIORITY JOURNAL; RECEPTOR GENE; RNA METABOLISM; TRANSCRIPTION REGULATION;

ANIMALIA;

EID: 0029846922     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Bauman, J. G. J., P. Bentvelzen: Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization. Cytometry 9, 517-524 (1988).
2. Baurén, G., L. Wieslander: Splicing of balbiani ring 1 gene pre-mRNA occurs simultaneously with transcription. Cell 76, 183-192 (1994).
3. Beyer, A. L., Y. N. Osheim: Splice site selection, rate of splicing, and alternative splicing on nascent transcripts. Genes & Dev. 2, 754-765 (1988).
4. Bond, C. M., B. Wold: Nucleolar localization of myc transcripts. Mol. Cell. Biol. 13, 3221-3230 (1993).
5. Carmo-Fonseca, M., D. Tollervey, R. Pepperkok, S. M. L. Barabino, A. Merder, C. Brunner, P. D. Zamore, M. R. Green, E. Hurt, A. I. Lamond: Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery. EMBO J. 10, 195-206 (1991).
6. Carmo-Fonseca, M., R. Pepperkok, B. S. Sproat, W. Ansorge, M. S. Swanson, A. I. Lamond: In vivo detection of snRNP-rich organelles in nuclei of mammalian cells. EMBO J. 10, 1863-1873 (1991).
7. Danscher, G.: Localization of gold in biological tissue. A photochemical method for light and electron microscopy. Histochemistry 71, 81-88 (1981).
8. De Lange, T., L. Shiue, R. M. Meyers, D. R. Cox, S. L. Naylor, A. M. Killery, H. E. Varmus: Structure and variability of human chromosome ends. Mol. Cell. Biol. 10, 518-527 (1990).
9. Den Hertog, J. D., S. W. de Laat, J. Schlessinger, W. Kruijer: Neuronal differentiation in response to epidermal growth factor of transfected murine P19 embryonal carcinoma cells expressing human epidermal growth factor receptors. Cell Growth Diff. 2, 155-164 (1991).
10. Dirks, R. W., F. M. van der Rijke, S. Fujihita, M. van der Ploeg, A. K. Raap: Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes. J. Cell Sci. 104, 1187-1197 (1993).
11. Egyhazi, E.: Inhibition of balbiani ring RNA synthesis at the initiation level. Proc. Natl. Acad. Sci. USA 72, 947-950 (1975).
12. Erickson, J. M., C. L. Rushford, D. J. Dorney, G. N. Wilson, R. D. Schmickel: Structure and variation of human ribosomal DNA; molecular analysis of cloned fragments. Gene 16, 1-19 (1981).
13. Feinberg, A. P., B. Vogelstein: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13 (1983).
14. Fu, X.-D., T. Maniatis: Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343, 437-441 (1990).
15. Haley, J., N. Whittle, P. Bennett, D. Kinchington, A. Ullrich, M. Waterfield: The human EGF-receptor gene: stucture of the 110 kb locus and identification of sequences regulating its transcription. Oncogene Res. 1, 375-396 (1987).
16. Höltke, H. J., C. Kessler: Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA based detection. Nucleic Acids Res. 18, 5843-5851 (1990).
17. Hozák, P., A. B. Hassan, D. A. Jackson, P. R. Cook: Visualization of replication factories attached to a nucleoskeleton. Cell 73, 361-373 (1993).
18. Huang, S., D. L. Spector: Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors. Genes & Dev. 5, 2288-2302 (1991).
19. Jackson, D. A., A. B. Hassan, R. J. Errington, P. R. Cook: Visualization of focal sites of transcription within human nuclei. EMBO J. 12, 1059-1065 (1993).
20. Jiménez-Garcia, L. F., D. L. Spector: In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73, 47-59 (1993).
21. Lawrence, J. B., R. H. Singer, L. M. Marselle: Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization. Cell 57, 493-502 (1989).
22. O'Keefe, R. T., S. C. Henderson, D. L. Spector: Dynamic organization of DNA replication in mammalian cell nuclei: spatial and temporally defined replication of chromosome-specific α-satellite DNA sequences. J. Cell Biol. 116, 1095-1110 (1992).
23. O'Keefe, R. T., A. Mayeda, C. L. Sadowski, A. R. Krainer, D. L. Spector: Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors. J. Cell Biol. 124, 249-260 (1994).
24. Raap, A. K., F. M. van der Rijke, R. W. Dirks, C. J. Sol, R. Boom, M. van der Ploeg: Bicolor fluorecence in situ hybridization to intron and exon mRNA sequences. Exp. Cell Res. 197, 319-322 (1991).
25. Rǎska, I., K. Koberna, M. Jarnik, V. Petrasovicova, J. Bednar, K. Rǎska, R. Bravo: Ultrastructural immunolocalization of cyclin/PCNA in synchronized 3T3 cells. Exp. Cell Res. 184, 81-89 (1989).
26. Shermoen, A. W., P. H. O'Farrell: Progression of the cell cycle through mitosis leads to abortion of nascent transcripts. Cell 67, 303-310 (1991).
27. Sibon, O. C. M., A. F. M. Cremers, J. Boonstra, B. M. Humbel, A. J. Verkleij: Localization of EGF-receptor mRNA in the nucleus of A431 cells by light microscopy. Cell Biol. Int. 17, 1-11 (1993).
28. Sibon, O. C. M., B. M. Humbel, A. De Graaf, A. J. Verkleij, A. F. M. Cremers: Ultrastructural localization of epidermal growth factor (EGF)-receptor transcripts in the cell nucleus using pre-embedding in situ hybridization in combination with ultra-small gold probes and silver enhancement. Histochemistry 101, 223-232 (1994).
29. Sibon, O C. M., A. F. M. Cremers, J. Boonstra, B. M. Humbel, A. J. Verkleij: Localization of nuclear RNA by pre- and post-embedding in situ hybridization using different gold probes. Histochemical J. 27, 35-45 (1995).
30. Spector, D. L.: Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. USA 87, 147-151 (1990).
31. Spector, D. L., X.-D. Fu, T. Maniatis: Association between distinct pre-mRNA splicing components and the cell nucleus. EMBO J. 10, 3467-3481 (1991).
32. Spector, D. L.: Macromolecular domains within the cell nucleus. Annu. Rev. Cell Biol. 9, 265-315 (1993).
33. Te Pas, M. F. W., J. Boonstra, L. M. Havekes, S. C. Hesseling, M. Ponec: The competence of transformed keratinocytes to differentiate is accompanied by amplification of the LDL- and EGF-receptor genes but not of the insulin receptor gene. Cell Biol. Int. Rep. 13, 237-249 (1989).
34. Ullrich, A., L. Coussens, J. S. Hayflick, T. J. Dull, A. Gray, A. W. Tam, J. Lee, Y. Yarden, T. A. Libermann, J. Schlessinger, J. Downward, E. L. V. Mayes, N. Whittle, M. D. Waterfield, P. H. Seeburg: Human epidermal growth factor receptor cDNA sequence and aberrant expression of amplified gene in A431 epidermoid carcinoma cells. Nature 309, 418-425 (1984).
35. Wansink, D. G., W. Schul, I. van der Kraan, B. van Steensel, R. van Driel, L. de Jong: Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus. J. Cell Biol. 122, 283-293 (1993).
36. Xing, Y., C. V. Johnson, P. R. Dobner, J. B. Lawrence: Higher level organization of individual gene transcription and RNA splicing. Science 259, 1326-1330 (1993).
37. Zhang, G., K. L. Taneja, R. H. Singer, M. R. Green: Localization of pre-mRNA splicing in mammalian nuclei. Nature 372, 809-812 (1994).