A method for co-localization of tubular lysosomes and microtubules in macrophages: Fluorescence microscopy of individual cells

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 10, 1996, Pages 1109-1114

  Robinson, John M ^a   Chiplonkar, Jayant ^a,b   Luo, Zhongrong ^a,c  

^a OHIO STATE UNIVERSITY   (United States)

^b Natl Facil Anim Tissue Cell C   (India)

^c American University of the Caribbean School of Medicine   (United States)

Author keywords

Fluorescence microscopy; Freeze substitution; Immunocytochemistry; Macrophages; Microtubules; Rapid freezing; Tubular lysosomes

Indexed keywords

STRUCTURAL PROTEIN;

ANIMAL CELL; ARTICLE; CELL ULTRASTRUCTURE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOSKELETON; FLUORESCENCE MICROSCOPY; IMMUNOCYTOCHEMISTRY; LYSOSOME; MACROPHAGE ACTIVATION; MICROTUBULE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA;

EID: 0029846906     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.10.8813075     Document Type: Article

References (22)

1. Araki N, Ogawa K (1987) Regulation of intracellular movements by the cytoskeletal system in rat alveolar macrophages. Acta Histochem Cytochem 20:659-678
2. Cooper MS, Cornell-Bell AH, Chernjavsky A, Dani JW, Smith SJ (1990) Tubulovesicular processes emerge from trans-Golgi cisternae, extend along microtubules, and interlink adjacent trans-Golgi elements into a reticulum. Cell 61:135-145
3. Dailey ME, Bridgman PC (1989) Dynamics of the endoplasmic reticulum and other membranous organelles in growth cones of cultured neurons. J Neurosci 9:1897-1909
4. Heuser J (1989a) Changes in lysosome shape and distribution correlated with changes in cytoplasmic pH. J Cell Biol 108:855-864
5. Heuser JE (1989b) Mechanisms behind the organization of membranous organelles in cells. Curr Opin Cell Biol 1:98-102
6. Hollenbeck PJ, Swanson JA (1990) Radial extension of macrophage tubular lysosomes supported by kinesin. Nature 346:864-866
7. Hopkins CR, Gibson A, Shipman M, Miller K (1990) Movement of internalized ligand-receptor complexes along a continuous endosomal reticulum. Nature 346:335-339
8. Lippincott-Schwartz J, Yuan L, Tipper C, Amherdt M, Orci L, Klausner RD (1991) Brefeldin A's effects on endosomes, lysosomes, and TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67:601-616
9. Luo Z, Robinson JM (1992) Co-localization of an endocytic marker and acid phosphatase in a tubular/reticular compartment in macrophages. J Histochem Cytochem 40:93-103
10. Melan MA (1994) Overview of cell fixation and permeabilization. Methods Mol Biol 34:55-66
11. Pettitt JM, Humphris CH, Barrett SP, Toh B-H, van Driel IR, Gleeson PA (1995) Fast freeze-fixation/freeze-substitution reveals the secretory membranes of the gastric parietal cell as a network of helically coiled tubules. A new model for parietal cell transformation. J Cell Sci 108:1127-1141
12. Pharie-Washington L, Wang E, Silverstein SC (1980) Phorbol myristate acetate stimulates pinocytosis and membrane spreading in mouse peritoneal macrophages. J Cell Biol 86:634-640
13. Robinson JM, Karnovsky MJ (1991) Rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity. J Histochem Cytochem 39:787-792
14. Robinson JM, Luo Z (1992) The association of microtubules with an acid phosphatase-containing tubular compartment in macrophages. Acta Histochem Cytochem 25:223-229
15. Robinson JM, Okada T, Castellot JJ Jr, Karnovsky MJ (1986) Unusual lysosomes in aortic smooth muscle cells: presence in living and rapidly frozen cells. J Cell Biol 102:1615-1622
16. Robinson JM, Vandré DD (1995) Stimulus-dependent alterations in macrophage microtubules: increased tubulin polymerization and detyrosination. J Cell Sci 108:645-655
17. Samuels AL, Giddings TH, Staehelin LA (1995) Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants. J Cell Biol 130:1345-1357
18. Swanson J, Bushnell A, Silverstein SC (1987) Tubular lysosome morphology and distribution within macrophages depend on the integrity of cytoplasmic microtubules. Proc Nad Acad Sci USA 84:1921-1925
19. Swanson JA, Locke A, Ansel P, Hollenbeck PJ (1992) Radial movement of lysosomes along microtubules in permeabilized macrophages. J Cell Sci 103:201-209
20. Terasaki M, Chen LB, Fujiwara K (1986) Microtubules and the endoplasmic reticulum are highly interdependent structures. J Cell Biol 103:1557-1568
21. Terasaki M, Reese TS (1992) Characterization of endoplasmic reticulum by co-localization of BiP and dicarbocyanine dyes. J Cell Sci 101:315-322
22. Wood SA, Brown WJ (1992) The morphology but not the function of endosomes and lysosomes is altered by brefeldin A. J Cell Biol 119:273-285