Random mutagenesis of Thermus aquaticus DNA polymerase I: Concordance of immutable sites in vivo with the crystal structure

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 18, 1996, Pages 9670-9675

  Suzuki, Motoshi ^a   Baskin, Dale ^a   Hood, Leroy ^a   Loeb, Lawrence A ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; DNA DIRECTED DNA POLYMERASE BETA;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENETICS; BINDING SITE; CRYSTAL STRUCTURE; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENETIC COMPLEMENTATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; THERMUS AQUATICUS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ARGININE; CODON; CRYSTALLOGRAPHY, X-RAY; DNA POLYMERASE I; ESCHERICHIA COLI; GLYCINE; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; PLASMIDS; PROTEIN CONFORMATION; THERMUS;

EID: 0029845979     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.18.9670     Document Type: Article

References (45)

1. Joyce, C. M. & Steitz, T. A. (1994) Annu. Rev. Biochem. 63, 777-822.
2. Kohlstaedt, L. A., Wang, J., Friedman, J. M., Rice, P. A. & Steitz, T. A. (1992) Science 256, 1783-1790.
3. Ollis, D. L., Brick, P., Hamlin, R., Xuong, N. G. & Steitz, T. A. (1985) Nature (London) 313, 762-766.
4. Sousa, R., Chung, Y. J., Rose, J. P. & Wang, B.-C. (1993) Nature (London) 364, 593-599.
5. Sawaya, M. R., Pelletier, H., Kumar, A., Wilson, S. H. & Kraut, J. (1994) Science 264, 1930-1935.
6. Davies, J. F., II, Almassy, R. J., Hostomska, Z., Ferre, R. A. & Hostomsky, Z. (1994) Cell 76, 1123-1133.
7. Pelletier, H., Sawaya, M. R., Kumar, A., Wilson, S. H. & Kraut, J. (1994) Science 264, 1891-1903.
8. Arnold, E., Jacobo-Molina, A., Nanni, R. G., Williams, R. L., Lu, X., Ding, J., Clark, A. D., Jr., Zhang, A., Ferris, A. L., Clark, P., Hizi, A. & Hughes, S. H. (1992) Nature (London) 357, 85-89.
9. Korolev, S., Nayal, M., Barnes, W. M., Di Cera, E. & Waksman, G. (1995) Proc. Natl. Acad. Sci. USA 92, 9264-9268.
10. Kim, Y., Eom, S. H., Wang, J., Lee, D.-S., Suh, S. W. & Steitz, T. A. (1995) Nature (London) 376, 612-616.
11. Kim, B. & Loeb, L. A. (1995) Proc. Natl. Acad. Sci. USA 92, 684-688.
12. Kim, B. & Loeb, L. A. (1995) J. Virol. 69, 6563-6566.
13. Sweasy, J. B. & Loeb, L. A. (1992) J. Biol. Chem. 267, 1407-1410.
14. Sweasy, J. B. & Loeb, L. A. (1993) Proc. Natl. Acad. Sci. USA 90, 4626-4630.
15. Sweasy, J. B., Chen, M. & Loeb, L. A. (1995) J. Bacteriol. 177, 2923-2925.
16. Kim, B., Hathaway, T. R. & Loeb, L. A. (1996) J. Biol. Chem. 271, 4872-4878.
17. Blank, A., Kim, B. & Loeb, L. A. (1994) Proc. Natl. Acad. Sci. USA 91, 9047-9051.
18. Witkin, E. M. & Roegner-Maniscalo, V. (1992) J. Bacteriol. 174, 4166-4168.
19. Takeshita, S., Sato, M., Toba, M., Masahashi, W. & Hashimoto-Gotoh, T. (1987) Gene 61, 63-74.
20. Lawyer, F. C., Stoffel, S., Saiki, R. K., Myambo, K., Drummond, R. & Gelfand, D. H. (1989) J. Blol. Chem. 264, 6427-6437.
21. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. USA 82, 488-492.
22. Dube, D. K., Parker, J. D., French, D. C., Cahill, D. S., Dube, S., Horwitz, M. S. Z., Munir, K. M. & Loeb, L. A. (1991) Biochemistry 30, 11760-11767.
23. Braithwaite, D. K. & Ito, J. (1993) Nucleic Acids Res. 21, 787-802.
24. Bryant, F. R., Johnson, K. A. & Benkovic, S. J. (1983) Biochemistry 22, 3537-3546.
25. Patel, P. H., Jacobo-Molina, A., Ding, J., Tantillo, C., Clark, A. D., Jr., Raag, R., Nanni, R. G., Hughes, S. H. & Arnold, E. (1995) Biochemistry 34, 5351-5363.
26. Mizrahi, V., Henrie, R. N., Marlier, J. F., Johnson, K. A. & Benkovic, S. J. (1985) Biochemistry: 24, 4010-4018.
27. Kornberg, A. & Baker, T. A. (1992) DNA Replication (Freeman, New York).
28. Beese, L. S., Friedman, J. M. & Steitz, T. A. (1993) Biochemistry 32, 14095-14101.
29. Blanco, L., Bernad, A., Blasco, M. A. & Salas, M. (1991) Gene 100, 27-38.
30. Delarue, M., Poch, O., Tordo, N., Moras, D. & Argos, P. (1990) Protein Eng. 3, 461-467.
31. Joyce, C. M., Kelley, W. S. & Grindley, N. D. F. (1982) J. Biol. Chem. 257, 1958-1964.
32. Ferrin, L. J. & Mildvan, A. S. (1985) Biochemistry 24, 6904-6913.
33. Basu, A. & Modak, M. J. (1987) Biochemistry 26, 1704-1709.
34. Rush, J. & Konigsberg, W. H. (1990) J. Biol. Chem. 265, 4821-4827.
35. Mullen, G. P., Shenbagamurthi, P. & Mildvan, A. S. (1989) J. Biol. Chem. 264, 19637-19647.
36. Bowie, J. U., Reidhaar-Olson, J. F., Lim, W. A. & Sauer, R. T. (1990) Science 247, 1306-1310.
37. Rennell, D., Bouvier, S. E., Hardy, L. W. & Poteete, A. R. (1991) J. Mol. Biol. 222, 67-88.
38. Pandey, V. N., Kaushik, N. & Modak, M. J. (1994) J. Biol. Chem. 269, 13259-13265.
39. Astatke, M., Grindley, N. D. F. & Joyce, C. M. (1995) J. Biol. Chem. 270, 1945-1954.
40. Osumi-Davis, P. A., de-Aguilera, M. C., Woody, R. W. & Woody, A.-Y. M. (1992) J. Mol. Biol. 226, 37-45.
41. Tabor, S. & Richardson, C. C. (1995) Proc. Natl. Acad. Sci. USA 92, 6339-6343.
42. Carroll, S. S., Cowart, M. & Benkovic, S. J. (1991) Biochemistry 30, 804-813.
43. Sousa, R. & Padilla, R. (1995) EMBO J. 14, 4609-4621.
44. Bonner, G., Patra, D., Lafer, E. M. & Sousa, R. (1992) EMBO J. 11, 3767-3775.
45. Pandey, V. N., Kaushik, N. & Modak, M. J. (1994) J. Biol. Chem. 269, 21828-21834.