Covalent attachment of FAD derivatives to a fusion protein consisting of 6-hydroxy-D-nicotine oxidase and a mitochondrial presequence. Folding, enzyme activity, and import of the modified protein into yeast mitochondria

Journal of Biological Chemistry

Volumn 271, Issue 41, 1996, Pages 25208-25212

  Stoltz, Michaela ^a   Rassow, Joachim ^a   Bückmann, Andreas F ^b   Brandsch, Roderich ^a,c  

^a Inst fur Biochem/Molekularbiologie   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FLAVINE ADENINE NUCLEOTIDE; MITOCHONDRIAL ENZYME; NICOTINE; OXIDOREDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SUBUNIT; MITOCHONDRIAL MEMBRANE; NEUROSPORA CRASSA; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; RETICULOCYTE; SACCHAROMYCES CEREVISIAE;

ANIMALS; DNA PRIMERS; FLAVIN-ADENINE DINUCLEOTIDE; HSP70 HEAT-SHOCK PROTEINS; MITOCHONDRIA; NEUROSPORA CRASSA; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; PROTEIN FOLDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTON-TRANSLOCATING ATPASES; RABBITS; RECOMBINANT FUSION PROTEINS; RETICULOCYTES; SACCHAROMYCES CEREVISIAE; TRYPSIN;

NEUROSPORA CRASSA; ORYCTOLAGUS CUNICULUS; SACCHAROMYCES CEREVISIAE;

EID: 0029845925     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.41.25208     Document Type: Article

References (23)

1. Decker, K., and Brandsch, R. (1991) Biofactors 3, 69-81
2. Brandsch, R., and Bichler, V. (1991) J. Biol. Chem. 266, 19056-19062
3. Stoltz, M., Rysavy, P., Kalousek, F., and Brandsch, R. (1995) J. Biol. Chem. 270, 8016-8022
4. Bückmann, A. F., Erdmann, H., Pietzsch, M., Hall, J. M., and Bannister, J. V. (1993) in Flavins and Flavoproteins (Yagi, K., ed) pp. 597-600, Walter de Gruyter & Co., Berlin
5. Brandsch, R., Bichler, V., and Krauss, B. (1989) Biochem. J. 258, 187-192
6. Gambill, D., Voos, W., Kang, P. J., Miao, B., Langer, T., Craig, E. A., and Pfanner, N. (1993) J. Cell Biol. 12, 3288-3296
7. Voos, W., von Ahsen, O., Müller, H., Guiard, B., Rassow, J., and Pfanner, N. (1996) EMBO J 15, 2668-2677
8. Pfanner, N., Tropschug, M., and Neupert, W. (1987) Cell 49, 815-823
9. Möhler, H., Brühmüller, M., and Decker, K. (1972) Eur. J. Biochem. 29, 152-155
10. Daum, G., Böhni, P. C., and Schatz, G. (1982) J. Biol. Chem. 257, 13028-13033
11. Hartl, F.-U., Ostermann, J., Guiard, B., and Neupert, W. (1987) Cell 51, 1027-1037
12. Mauch, L., Bichler, V., and Brandsch, R. (1989) FEBS Lett. 257, 86-88
13. Kang, P. J., Ostermann, J., Shilling, J., Neupert, W., Craig, E. A., and Pfanner, N. (1990) Nature 348, 137-143
14. Rassow, J., Hartl, F.-U., Guiard, B., Pfanner, N., and Neupert, W. (1990) FEBS Lett. 275, 190-194
15. Kim, J., Fuller, J. H., Cecchini, G., and McIntire, W. S. (1994) J. Bacteriol. 176, 6349-6361
16. Scrutton, N. S., Packman, L. C., Mathews, F. S., Rohlfs, R. J., and Hille, R. (1994) J. Biol. Chem. 269, 13942-13950
17. Zhou, B. P., Lewis, D. A., Kwan, S.-W., Kirksey, T. T., and Abell, C. W. (1995) Biochemistry 34, 9526-9531
18. Karplus, P. A., Daniels, M. J., and Herriott, J. R. (1991) Science 251, 60-66
19. Kim, J. J. P., Wang, M., and Paschke, R. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 7523-7527
20. Hartl, F.-U., and Neupert, W. (1990) Science 247, 930-938
21. Voos, W., Gambill, B. D., Guiard, B., Pfanner, N., and Craig, E. A. (1993) J. Cell Biol. 123, 119-126
22. Zara, V., Palmieri, F., Mahlke, K., and Pfanner, N. (1992) J. Biol. Chem. 267, 12077-12081
23. Mayer, A., Neupert, W., and Lill, R. (1995) Cell 80, 127-137