Mutations in active-site residues of the uracil-DNA glycosylase encoded by vaccinia virus are incompatible with virus viability

Journal of Virology

Volumn 70, Issue 11, 1996, Pages 7965-7973

  Ellison, Kimberly S ^a   Peng, Wei ^a   McFadden, Grant ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b ALBERTA ENVIRONMENT AND PARKS   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; MUTANT PROTEIN; URACIL DNA GLYCOSYLTRANSFERASE; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA REPLICATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GENE MUTATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; VACCINIA VIRUS; VIRUS GENE; VIRUS RECOMBINATION;

EID: 0029845801     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.11.7965-7973.1996     Document Type: Article

References (55)

1. Bennett, S. E., and D. W. Mosbaugh. 1992. Characterization of the Eschcrichia coli uracil-DNA glycosylase inhibitor protein complex. J. Biol. Chem. 267:22512-22521.
2. Caradonna, S., D. Worrad, and R. Lirette. 1987. Isolation of a herpes simplex virus cDNA encoding the DNA repair enzyme uracil-DNA glycosylase. J. Virol. 61:3040-3047.
3. Carpenter, M. S., and A. M. DeLange. 1991. A temperature-sensitive lesion in the small subunit of the vaccinia virus-encoded mRNA capping enzyme causes a defect in viral telomere resolution. J. Virol. 65:4042-4050.
4. Chee, M. S., A. T. Bankier, S. Beck, R. Bohni, C. M. Brown, R. Cerny, T. Horsnell, C. A. I. Hutchison, T. Kouzarides, J. A. Martignetti, E. Preddie, S. C. Satchwell, P. Tomlinson, K. M. Weston, and B. G. Barrell. 1990. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr. Top. Microbiol. Immunol. 154:125-170.
5. Cone, R., J. Duncan, L. Hamilton, and E. C. Friedberg. 1977. Partial purification and characterization of a uracil DNA N-glycosidase from Bacillus subtilis. Biochemistry 16:3194-3201.
6. DeLange, A. M., and G. McFadden. 1986. Sequence-nonspecific replication of transfected plasmid DNA in poxvirus-infected cells. Proc. Natl. Acad. Sci. USA 83:614-618.
7. DeLange, A. M., and G. McFadden. 1990. The role of telomeres in poxvirus DNA replication. Curr. Top. Microbiol. Immunol. 163:71-92.
8. Du, S., and P. Traktman. 1996. Vaccinia virus DNA replication: 200 bp of telomeric sequence confer optimal replication efficiency on minichromosome templates. Proc. Natl. Acad. Sci. USA 93:9693-9698.
9. Duncan, B. K. 1985. Isolation of insertion, deletion, and nonsense mutations of the uracil-DNA glycosylase (ung) gene of Escherichia coli K-12. J. Bacteriol. 164:689-695.
10. Ellison, K., X. Xu, and G. McFadden. 1996. Unpublished observations.
11. Focher, F., P. Mazzarello, A. Verri, U. Hubscher, and S. Spadari. 1990. Activity profiles of enzymes that control the uracil incorporation into DNA during neuronal development. Mutat. Res. 237:65-73.
12. Focher, F., A. Verri, S. Verzeletti, P. Mazzarello, and S. Spadari. 1992. Uracil in oriS of herpes simplex 1 alters its specific recognition by origin binding protein (OBP): does virus induced uracil-DNA glycosylase play a key role in viral reactivation and replication? Chromosoma 102:S67-S71.
13. Goebel, S. J., G. P. Johnson, M. E. Perkus, S. W. Davis, J. P. Winslow, and E. Paoletti. 1990. The complete DNA sequence of vaccinia virus. Virology 179:247-266.
14. Graham, K, A., A. Opgenorth, C. Upton, and G. McFadden. 1992. Myxoma virus M11L ORF encodes a protein for which cell surface localization is critical in manifestation of viral virulence. Virology 191:112-124.
15. Karran, P., R. Cone, and E. C. Friedberg. 1981. Specificity of the bacteriophage PBS2 induced inhibitor of uracil-DNA glycosylase. Biochemistry 20: 6092-6096.
16. Mackett, M., G. L. Smith, and B. Moss. 1985. The construction and characterisation of vaccinia virus recombinants expressing foreign genes, p. 191-211. In D. M. Glover (ed.), DNA cloning: a practical approach, vol. 2. IRL Press, Oxford.
17. McFadden, G., and S. Dales. 1980. Biogenesis of poxviruses: preliminary characterization of conditional lethal mutants of vaccinia virus defective in DNA synthesis. Virology 103:68-79.
18. McGeoch, D. J., M. A. Dalrymple, A. J. Davison, A, Dolan, M. C. Frame, D. McNab, L. J. Perry, J. E. Scolt, and P. Taylor. 1988. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. J. Gen. Virol. 69:1531-1574.
19. Meyer-Siegler, K., D. J. Mauro, G. Seal, J. Wurzer, J. K. deRiel, and M. A. Sirover. 1991. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc. Natl. Acad. Sci. USA 88:8460-8464.
20. Millns, A. K., M. S. Carpenter, and A. M. DeLange. 1994. The vaccinia virus-encoded uracil DNA glycosylase has an essential role in viral DNA replication. Virology 198:504-513.
21. Mol, C. D., A. S. Arvai, R. J. Sanderson, G. Slupphaug, B. Kavli, H. E. Krokan, D. W. Mosbaugh, and J. A. Tainer. 1995. Crystal structure of human uracil-DNA glycosylase in complex with a protein inhihitor: protein mimicry of DNA. Cell 82:701-708.
22. Mol, C. D., A. S. Arvai, G. Slupphaug, B. Kavli, I. Alseth, H. E. Krokan, and J. A. Tainer. 1995. Crystal structure and mutational analysis of human uracil-DNA glycosylase: structural basis for specificity and catalysis. Cell 80:869-878.
23. Morgan, A. R., and J. Chlebek. 1988. Quantitative assays for uracil-DNA glycosylase of high sensitivity. Biochem. Cell Biol. 66:157-160.
24. Moss, B. 1990. Poxviridae and their replication, p. 2079-2111. In B. N. Fields and D. M. Knipe (ed.), Virology, 2nd ed. Raven Press, New York.
25. Moss, B. 1990. Regulation of vaccinia virus transcription. Annu. Rev. Biochem. 59:661-688.
26. Moss, B., B.-Y. Ahn, B. Amegadzie, P. D. Gershon, and J. G. Keck. 1991. Cytoplasmic transcription system encoded by vaccinia virus. J. Biol. Chem. 266:1355-1358.
27. Mossman, K., P. Nation, J. Macen, M. Garbutt, A. Lucas, and G. McFadden. 1996. Myxoma virus M-T7, a secreted homolog of the interferon-γ receptor, is a critical virulence factor for the development of myxomatosis in European rabbits. Virology 215:17-30.
28. Moyer, R. W., and R. L. Graves. 1981. The mechanism of cytoplasmic orthopoxvirus DNA replication. Cell 27:391-401.
29. Mullaney, J., H. W. Moss, and D. J. McGeoch. 1989. Gene UL2 of herpes simplex virus type 1 encodes a uracil DNA glycosylase. J. Gen. Virol. 70: 449-454.
30. Muller, S. J., and S. Caradonna. 1993. Cell cycle regulation of a human cyclin-like gene encoding uracil-DNA glycosylase. J. Biol. Chem. 268:1310-1319.
31. Nagelhus, T. A., G. Slupphaug, T. Lindmo, and H. E. Krokan. 1995. Cell cycle regulation and subcellular localization of the major human uracil-DNA glycosylase. Exp. Cell Res. 220:292-297.
32. Niles, E. G., R. C. Condit, P. Caro, K. Davidson, L. Matusick, and J. Seto. 1986. Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment. Virology 153:96-112.
33. Olsen, L. C., R. Aasland, C. U. Wittwer, H. E. Krokan, and D. E. Heiland. 1989. Molecular cloning of human uracil-DNA glycosylase. a highly conserved DNA repair enzyme. EMBO J. 8:3121-3125.
34. Percival, K. J., M. B. Klein, and P. M. Burgers. 1989. Molecular cloning and primary structure of the uracil DNA glycosylase gene from Saccharomyces cerevisiae. J. Biol. Chem. 264:2593-2598.
35. Pogo, B. G. 1977. Elimination of naturally occurring crosslinks in vaccinia virus DNA after viral penetration into cells. Proc. Natl. Acad. Sci. USA 74:1739-1742.
36. Pogo, B. G., M. O'Shea, and P. Freimuth. 1981. Initiation and termination of vaccinia virus DNA replication. Virology 108:241-248.
37. Pogo, B. G. T., E. M. Berkowitz, and S. Dales. 1984. Investigation of vaccinia virus DNA replication employing a conditional lethal mutant defective in DNA. Virology 132:436-444.
38. Prichard, M. N., G. M. Duke, and E. S. Mocarski. 1996. Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication. J. Virol. 70:3018-3025.
39. Pyles, R. B., and R. L. Thompson. 1994. Evidence that the herpes simplex virus type 1 uracil DNA glycosylase is required for efficient viral replication and latency in the murine nervous system. J. Virol. 68:4963-4972.
40. Sato, S., T. Yamamoto, Y. Isegawa, and K.Yamanishi. 1994. Identification of human herpesvirus 6 uracil-DNA glycosylase gene. J. Gen. Virol. 75:2349-2354.
41. Savva, R., K. McAuley-Hecht, T. Brown, and L. Pearl. 1995. The structural basis of specific base-excision repair by uracil-DNA glycosylase. Nature (London) 373:487-493.
42. Seal, G., and M. A. Sirover. 1986. Physical association of the human base-excision repair enzyme uracil DNA glycosylase with the 70,000-dalton catalytic subunit of DNA polymerase alpha. Proc. Natl. Acad. Sci. USA 83:7608-7612.
43. Singhal, R. K., R. Prasad, and S. H. Wilson. 1995. DNA polymerase beta conducts the gap-filling step in uracil-initiated base excision repair in a bovine testis nuclear extract. J. Biol. Chem. 270:949-957.
44. Slupphaug, G., I. Eftedal, B. Kavli, S. Bharati, N. M. Helle, T. Haug, D. W. Levine, and H. E. Krokan. 1995. Properties of a recombinant human uracil-DNA glycosylase from the UNG gene and evidence that UNG encodes the major uracil-DNA glycosylase. Biochemistry 34:128-138.
45. Sobol, R. W., J. K. Horton, R. Kuhn, H. Gu, R. K. Singhal, R. Prasad, K. Rajewsky, and S. H. Wilson. 1996. Requirement of mammalian DNA polymerase-beta in base excision repair. Nature (London) 379:183-186.
46. Stuart, D. T., C. Upton, M. A. Higman, E. G. Niles, and G. McFadden. 1993. A poxvirus-encoded uracil DNA glycosylase is essential for virus viability. J. Virol. 67:2503-2512.
47. Tartaglia, J., J. Winslow, S. Goebel, G. P. Johnson, J. Taylor, and E. Paoletti. 1990. Nucleotide sequence analysis of a 10.5 kbp HindIII fragment of fowlpox virus: relatedness to the central portion of the vaccinia virus HindIII D region. J. Gen. Virol. 71:1517-1524.
48. Tomilin, N. V., and O. N. Aprelikova. 1989. Uracil-DNA glycosylases and DNA uracil repair. Int. Rev. Cytol. 114:125-179.
49. Traktman, P. 1996. Poxvirus DNA replication, p. 775-798. In M. DePamphilis (ed.), DNA replication in eukaryotic cells. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N.Y.
50. Traktman, P. 1990. Poxviruses: an emerging portrait of biological strategy. Cell 62:621-626.
51. Upton, C., D. T. Stuart, and G. McFadden. 1993. Identification of a poxvirus gene encoding a uracil DNA glycosylase. Proc. Natl. Acad. Sci. USA 90: 4518-4522.
52. Verri, A., S. Verzeletti, P. Mazzarello, S. Spadari, M. Negri, G. Bunone, V. G. Della, U. Hubscher, and F. Focher. 1992. DNA synthesis enzymes and proliferating cell nuclear antigen in normal and neoplastic nerve cells. Anticancer Res. 12:1099-1105.
53. Wang, Z., and D. W. Mosbaugh. 1989. Uracil-DNA glycosylase inhibitor gene of bacteriophage PBS2 encodes a binding protein specific for uracil-DNA glycosylase. J. Biol. Chem. 264:1163-1171.
54. Worrad, D. M., and S. Caradonna. 1988. Identification of the coding sequence for herpes simplex virus uracil-DNA glycosylase. J. Virol. 62:4774-4777.
55. Yáñez, R. J., J. M. Rodríguez, M. L. Nogal, L. Yuste, C. Enríquez, J. F. Rodríguez, and E. Vin̄uela. 1995. Analysis of the complete nucleotide sequence of African swine fever virus. Virology 208:249-278.