Cloning and characterization of two Serratia marcescens genes involved in core lipopolysaccharide biosynthesis?

Journal of Bacteriology

Volumn 178, Issue 19, 1996, Pages 5741-5747

  Guasch, Joan Francesc ^a   Piqué, Nuria ^a   Climent, Nuria ^a   Ferrer, Santiago ^a   Merino, Susana ^a   Rubires, Xavier ^a   Tomas, Juan M ^a   Regué, Miguel ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

3 DEOXY MANNO OCTULOSONIC ACID; BACTERIOCIN; BACTERIUM LIPOPOLYSACCHARIDE; DNA FRAGMENT;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; CARBOHYDRATE SYNTHESIS; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SERRATIA MARCESCENS; SOUTHERN BLOTTING;

EID: 0029844673     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.19.5741-5747.1996     Document Type: Article

References (32)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Austin, E. A., J. F. Graves, L. A. Hite, C. T. Parker, and C. L. Schnaitman. 1990. Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus. J. Bacteriol. 172:5312-5325.
3. Belunis, C. J., K. E. Mdluli, C. R. H. Raetz, and F. E. Nano. 1992. A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specific epitope. J. Biol. Chem. 267:18702-18707.
4. Chow, W. Y., and D. E. Berg. 1988. Tn5tac1, a derivative of Tn5 that generates conditional mutations. Proc. Natl. Acad. Sci. USA 85:6468-6472.
5. Clementz, T., and C. R. H. Raetz. 1991. A gene coding for 3-deoxy-D-mannooctulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing. J. Biol. Chem. 266:9687-9696.
6. Enfedaque, J., S. Ferrer, J. F. Guasch, J. Tomás, and M. Regué. 1996. Bacteriocin 28b from Serratia marcescens N28b: identification of Escherichia coli surface components involved in bacteriocin binding and translocation. Can. J. Microbiol. 42:19-26.
7. Felsenstein, J. 1989. PHYLIP: phylogeny inference package (version 3.2). Cladistics 5:164-166,
8. Ferrer, S., M. B. Viejo, J. F. Guasch, J. Enfedaque, and M. Regué, 1996. Genetic evidence for an activator required for induction of colicin-like bactericin 28b production in Serratia marcescens by DNA-damaging agents. J. Bacteriol. 178:951-960.
9. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Keriavage, C. J. Bult, J.-F. Tomb, B. A. Dougherty, J. M. Merrick, K. MacKenney, G. Sutton, W. FitzHugh, C. Fields, J. D. Gocayne, J. Scott, R. Shlrley, L.-I. Liu, A. Glodek, J. M. Kelley, J. F. Weldman, C. A. Philips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Uterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrmann, N. S. M. Geoghagen, C. L. Gnehm, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
10. Gargallo-Vlola, D. V. 1989. Enzyme polymorphism, prodigiosin production, and plasmid fingerprints in clinical and naturally occurring isolates of Serratia marcescens, J. Clin. Microbiol. 27:860-868.
11. Guasch, J. F., J. Enfedaque, S. Ferrer, D. Gargallo, and M. Regué. 1995. Bacteriocin 28b, a chromosomally encoded bacteriocin produced by most Serratia marcescens biotypes. Res. Microbiol. 146:477-483.
12. Guasch, J. F., S. Ferrer, J. Enfedaque, M. B. Viejo, and M. Regué. 1995. A 17 kDa outer-membrane protein (Omp4) from Serratia marcescens confers partial resistance to bacteriocin 28b when expressed in Escherichia coli. Microbiology 141:2535-2542.
13. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids, J. Mol. Biol. 166:557-580.
14. Klena, J. D., E. Pradel, and C. A. Schnaitman. 1992. Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaI, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium. J. Bacteriol. 174:4746-4752.
15. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
16. Löbau, S., U. Mamat, W. Brabetz, and H. Brade. 1995. Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-α-D-manno-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183. Mol. Microbiol. 18:391-399.
17. Mamat, U., M. Baumann, G. Schmidt, and H. Brade. 1993. The genus-specific lipopolysaccharide epitope of Chlamydia is assembled in C. psittaci and C. trachomatis by glycosyltransferases of low homology. Mol. Microbiol. 10:935-941.
18. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Osborn, M. J. 1963. Studies of the Gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctanoate in lipolysaccharide of Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 50:499-506.
20. Parker, C. T., E. Pradel, and C. A. Schnaitman. 1992. Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ. rfaP, and rfaG of Escherichia coli K-12. J. Bacteriol. 174:930-934.
21. Pradel, E., C. T. Parker, and C. A. Schnaitman. 1992. Structure of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core. J. Bacteriol. 174:4736-4745.
22. Pradel, E., and C. A. Schnaitman. 1991. Effect of the rfaH (sfrB) and temperature on the expression of rfa genes of Escherichia coli K-12. J. Bacteriol. 173:6428-6431.
23. Pugsley, A. P., and B. Oudega. 1987. Methods for studying colicins and their plasmids, p. 105-161. In K. G. Hardy (ed.), Plasmids, a practical approach. IRL Press, Oxford.
24. Radzlejewska-Lebrecht, J., D. Krajewska-Pietrasik, and H. Mayer. 1990. Terminal and chain-linked residues of D-galacturonic acid: characteristic constituents of the R-core regions of Proteae and of Serratia marcescens. Syst. Appl. Microbiol. 13:214-219.
25. Roncero, C., and M. J. Casadaban, 1992. Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus. J. Bacteriol. 174:3250-3260.
26. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
27. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
28. Schnaitman, C. L., and J. D. Klena. 1993. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol. Rev. 57:655-682.
29. Vlejo, M. B., J. Enfedaque, J. F. Guasch, S. Ferrer, and M. Regué. 1995. Protection against bacteriocin 28b in Serratia marcescens is apparently not related to the expression of an immunity gene. Can. J. Microbiol. 41:217-226.
30. Viejo, M. B., S. Ferrer, J. Enfedaque, and M. Regué. 1992, Cloning and DNA sequence analysis of a bacteriocin gene from Serratia marcescens. J. Gen. Microbiol. 138:1737-1743.
31. Viljanen, P., and M. Vaara. 1984. Susceptibility of gram-negative bacteria to polymyxin B nonapeptide. Antimicrob. Agents Chemother. 25:701-705.
32. Weisbach, A., and J. Hurwitz. 1959. The formation of 2-keto-3-deoxyheptanoic acid in extracts of E. coli B. J. Biol. Chem. 234:705-712.