An improved diffusion cell design for determining drug transport parameters across cultured cell monolayers

Journal of Pharmaceutical Sciences

Volumn 85, Issue 11, 1996, Pages 1196-1203

  Imanidis, Georg ^a   Waldner, Christoph ^a   Mettler, Christoph ^a   Leuenberger, Hans ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

MANNITOL; POLYCARBONATE; SALICYLIC ACID; TESTOSTERONE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DIFFUSION; DRUG PENETRATION; DRUG TRANSPORT; KIDNEY CELL; MEMBRANE PERMEABILITY; NONHUMAN; SWINE;

EID: 0029844399     PISSN: 00223549     EISSN: None     Source Type: Journal    
DOI: 10.1021/js960102g     Document Type: Article

References (20)

1. Audus, K. L.; Bartel, R. L.; Hidalgo, I. J.; Borchardt, R. T. The use of cultured epithelial and endothelial cells for drug transport and metabolism studies. Pharm. Res. 1990, 7, 435-451.
2. Pharmaceutical Applications of Cell and Tissue Culture to Drug Transport. NATO ASI Series; Wilson, G.; Davis, S. S.; Ilium, L.; Zweibaum, A., Eds. Plenum: New York and London, 1991.
3. Tsuji, A.; Takanaga, H.; Tamai, I.; Terasaki, T. Transcellular transport of benzoic acid across Caco-2 cells by a pH-dependent and carrier-mediated transport mechanism. Pharm. Res. 1994, 11, 30-37.
4. Rubas, W.; Jezyk, N.; Grass, G. M. Comparison of the permeability characteristics of a human colonic epithelial (Caco-2) cell line to colon of rabbit, monkey and dog intestine and human drug absorption. Pharm. Res. 1993, 10, 113-117.
5. Hilgers, A. R.; Conradi, R. A.; Burton, P. S. Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa. Pharm. Res. 1990, 7, 902-910.
6. Conradi, R. A.; Hilgers, A. R.; Ho, N. F. H.; Burton, P. S. The influence of peptide structure on transport across Caco-2 cells. Pharm. Res. 1991, 8, 1453-1460.
7. Karlsson, J.; Artursson, P. A method for the determination of cellular permeability coefficients and aqueous boundary layer thickness in monolayers of intestinal epithelial (Caco-2) cells grown in permeable filter chambers. Int. J. Pharm. 1991, 71, 55-64
8. Hidalgo, I. J.; Hillgren, K. M.; Grass, G. M.; Borchardt, R. T. Characterization of the unstirred water layer in Caco-2 cell monolayers using a novel diffusion apparatus. Pharm. Res. 1991, 8, 222-227.
9. Grass, G. M.; Sweetana, S. A. In vitro measurement of gastrointestinal tissue permeability using a new diffusion cell. Pharm. Res. 1988, 5, 372-376.
10. Ho, N. F. H.; Raub, T. J.; Burton, P. S.; Barsuhn, C. L.; Adson, A.; Audus, K. L.; Borchardt, R. T. Quantitative approaches to delineate passive transport mechanisms in cell culture monolayers. In Transport Processes in Pharmaceutical Systems; Himmelstein, K. J.; Amidon, G. L.; Lee, P. I., Eds.; Marcel Dekker: New York, in press.
11. Komiya, I.; Park, J. Y.; Kamani, A.; Ho, N. F. H.; Higuchi, W. I. Quantitative mechanistic studies in simultaneous fluid flow and intestinal absorption using steroids as model solutes. Int. J. Pharm. 1980, 4, 249-262.
12. Högerle, M. L.; Winne, D. Drug absorption by the rat jejunum perfused in situ. Naunyn-Schmiedeberg's Arch. Pharmacol. 1983, 322, 249-255.
13. Leuenberger, H.; Buchmann, S.; Reinke, C.; Schmid, B. An in vitro absorption model system based on cell monolayers (Intestine 407, MDBK). In Pharmaceutical Applications of Cell and Tissue Culture to Drug Transport, NATO ASI Series; Wilson, G.; Davis, S. S.; Illum, L.; Zweibaum, A., Eds.; Plenum: New York and London, 1991; pp 121-139.
14. Leuenberger, H.; Buchmann, S.; Reinke, C. Membrane system and process for testing substance diffusion through and method for making the membrane. U.S. Patent 4 812 407 1989.
15. Cohick, W. S.; Clemmons, D. R. Enhanced expression of dihydrofolate reductase by bovine kidney epithelial cells results in altered cell morphology, IGF-I responsiveness and IGF binding protein-3 expression. J. Cell. Physiol. 1994, 161, 178-186.
16. Levich, V. G. Physicochemical Hydrodynamics. Prentice-Hall: Englewood Cliffs, NJ, 1962; pp 60-72.
17. Cereijido, M.; Robbins, E. S.; Dolan, W. J.; Rotunno, C. A.; Sabatini, D. D. Polarized monolayers formed by epithelial cells on a permeable and translucent support. J. Cell Biol. 1978, 77, 853-880.
18. Amidon, G. E.; Higuchi, W. I.; Ho, N. F. H. Theoretical and experimental studies of transport of micelle-solubilized solutes. J. Pharm. Sci. 1982, 71, 77-84.
19. Adson, A.; Burton, P. S.; Raub, T. J.; Barsuhn, C. L.; Audus, K. L.; Ho, N. F. H. Passive diffusion of weak organic electrolytes across Caco-2 cell monolayers: Uncoupling the contributions of hydrodynamic, transcellular, and paracellular barriers. J. Pharm. Sci. 1995, 84, 1197-1204.
20. Hartiala, K. Metabolism of hormones, drugs and other substances by the gut. Physiol. Rev. 1973, 53, 496-534.