Analysis of connexin43 expression under the control of a metallothionein promoter

Cell Growth and Differentiation

Volumn 7, Issue 10, 1996, Pages 1403-1413

  Bechberger, John F ^a   Khoo, Nelson S K ^a   Naus, Christian C G ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CADMIUM; CONNEXIN 43; METALLOTHIONEIN; ZINC;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; CELL GROWTH; CELL PROLIFERATION; CONTROLLED STUDY; GENE CONTROL; GENE EXPRESSION REGULATION; GLIOMA CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RAT;

ANIMALS; CONNEXIN 43; GENE EXPRESSION REGULATION; GENE TRANSFER TECHNIQUES; METALLOTHIONEIN; PROMOTER REGIONS (GENETICS); RATS; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0029842627     PISSN: 10449523     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (46)

1. Loewenstein, W. R., and Kanno, Y. Intercellular communication and tissue growth. I. Cancerous growth. J. Cell Biol., 33: 225-234, 1967.
2. Mesnil, M., and Yamasaki, H. Cell-cell communication and growth control of normal and cancer cells: evidence and hypothesis. Mol. Carcinog., 7: 14-17, 1993.
3. Holder, J. W., Elmore, E., and Barrett, J. C. Gap junction function and cancer. Cancer Res., 53: 3475-3485, 1993.
4. Hotz-Wagenblatt, A., and Shalloway, D. Gap junctional communication and neoplastic transformation. Crit. Rev. Oncog., 4: 541-558, 1993.
5. Ruch, R. J. The role of gap junctional intercellular communication in neoplasia. Ann. Clin. Lab. Sci., 24: 216-231, 1994.
6. Yamasaki, H., and Naus, C. C. G. Role of connexin genes in growth control. Carcinogenesis, 17: 1199-1213, 1996.
7. Krutovskikh, V., Mazzoleni, G., Mironov, N., Omori, Y., Aguelon, A. M., Mesnil, M., Berger, F., Partensky, C., and Yamasaki, H. Altered homologous and heterologous gap-junctional intercellular communication in primary human liver tumors associated with aberrant protein localization but not gene mutation of connexin 32. Int. J. Cancer, 56: 87-94, 1994.
8. Tomasetto, C., Neveu, M. J., Daley, J., Horan, P. K., and Sager, R. Specificity of gap junction communication among human mammary cells and connexin transfectants in culture. J. Cell Biol., 122: 157-167, 1993.
9. Krutovskikh, V. A., Oyamada, M., and Yamasaki, H. Sequential changes of gap-junctional intercellular communications during multistage rat liver carcinogenesis: direct measurement of communication in vivo. Carcinogenesis, 12: 1701-1706, 1991.
10. Enomoto, T., and Yamasaki, H. Lack of intercellular communication between chemically transformed and surrounding nontransformed BALB/c 3T3 cells. Cancer Res., 44: 5200-5203, 1984.
11. Hamada, J., Takeichi, N., and Kobayashi, H. Metastatic capacity and intercellular communication between normal cells and metastatic cell clones derived from a rat mammary carcinoma. Cancer Res., 48: 5129-5132, 1988.
12. Ren, J., Hamada, J-I., Takeichi, N., Fujikawa, S., and Kobayashi, H. Ultrastructural differences in junctional intercellular communication between highly and weakly metastatic clones derived from rat mammary carcinoma. Cancer Res., 50: 358-362, 1990.
13. Zhu, D., Caveney, S., Kidder, G. M., and Naus, C. C. G. Transfection of C6 glioma cells with connexin 43 cDNA: analysis of expression, intercellular coupling, and cell proliferation. Proc. Natl. Acad. Sci. USA, 88: 1883-1887, 1991.
14. Mesnil, M., Krutovskikh, V., Piccoli, C., Elfgang, C., Traub, O., Willecke, K., and Yamasaki, H. Negative growth control of HeLa cells by connexin genes: connexin species specificity. Cancer Res., 55: 629-639, 1995.
15. Mehta, P. P., Hotz-Wagenblatt, A., Rose, B., and Shalloway, D. Incorporation of the gene for a cell-cell channel protein into transformed cells leads to normalization of growth. J. Membr. Biol., 124: 207-225, 1991.
16. Rose, B., Mehta, P. P., and Loewenstein, W. R. Gap-junction protein gene suppresses tumorigenicity. Carcinogenesis, 14: 1073-1075, 1993.
17. Bond, S. L., Bechberger, J. F., Khoo, N. K. S., and Naus, C. C. G. Transfection of C6 glioma cells with connexin32: the effects of expression of a nonendogenous gap junction protein. Cell Growth & Differ., 5: 179-186, 1994.
18. Eghbali, B., Kessler, J. A., Reid, L. M., Roy, C., and Spray, D. C. Involvement of gap junctions in tumorigenesis: transfection of tumor cells with connexin32 cDNA retards growth in vivo. Proc. Natl. Acad. Sci. USA, 88: 10701-10705, 1991.
19. Benda, P., Lightbody, J., Sato, G., Levine, L., and Sweet, W. Differentiated rat glial cell strain in tissue culture. Science (Washington DC), 161: 370-371, 1968.
20. Naus, C. C. G., Zhu, D., Todd, S. D. L., and Kidder, G. M. Characterization of C6 glioma cells overexpressing gap junction protein. Cell. Mol. Neurobiol., 12: 163-175, 1992.
21. Naus, C. C. G., Elisevich, K., Zhu, D., Belliveau, D. J., and Del Maestro, R. F. In vivo growth of C6 glioma cells transfected with connexin43 cDNA. Cancer Res., 52: 4208-4213, 1992.
22. McNeall, J., Sanchez, A., Gray, P. P., Chesterman, C. N., and Sleigh, M. J. Hyperinducible gene expression from a metallothionein promoter containing additional metallo-responsive elements. Gene, 76: 81-88, 1989.
23. Subramani, M., and Southern, P. Analysis of gene expression using simian virus 40 vectors. Anal. Biochem., 135: 1-15, 1983.
24. Naus, C. C. G., Bechberger, J. F., Caveney, S., and Wilson, J. X. Expression of gap junction genes in astrocytes and C6 glioma cells. Neurosci. Lett., 126: 33-36, 1991.
25. Auer, R., Del Maestro, R. F., and Anderson, R. A simple and reproducible experimental in vivo glioma model. Can. J. Neurol., 8: 325-331, 1981.
26. Zhu, D., Kidder, G. M., Caveney, S., and Naus, C. C. G. Growth retardation in glioma cells cocultured with cells overexpressing a gap junction protein. Proc. Natl. Acad. Sci. USA, 89: 10218-10221, 1992.
27. Musil, L. S., Cunningham, B. A., Edelman, G. M., and Goodenough, D. A. Differential phosphorylation of the gap junction protein connexin43 in junctional communication-competent and -deficient cell lines. J. Cell Biol., 111: 2077-2088, 1990.
28. Musil, L. S., and Goodenough, D. A. Gap junctional intercellular communication and the regulation of connexin expression and function. Curr. Opin. Cell Biol., 2: 875-880, 1990.
29. Musil, L. S., and Goodenough, D. A. Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques. J. Cell Biol., 115: 1357-1374, 1991.
30. Jou, Y. S., Matesic, D., Dupont, E., Lu, S. C., Rupp, H. L., Madhukar, B. V., Oh, S. Y., Trosko, J. E., and Chang, C. C. Restoration of gapjunctional intercellular communication in a communication-deficient rat liver cell mutant by transfection with connexin 43 cDNA. Mol. Carcinog., 8: 234-244, 1993.
31. Bradshaw, S. L., Naus, C. C. G., Zhu, D., Kidder, G. M., and Han, V. K. Insulin-like growth factor binding protein-4 gene expression is induced by transfection of gap junction connexin43 gene in a C6 glioma cell line. Growth Regul., 3: 26-29, 1993.
32. Swanson, R. A., and Sharp, F. R. Zinc toxicity and induction of the 72 kD heat shock protein in primary astrocyte culture. Glia, 6: 198-205, 1992.
33. Thiele, D. J. Metal-regulated transcription in eukaryotes. Nucleic Acids Res., 20: 1183-1191, 1992.
34. Brewer, G. Calmodulin, zinc and calcium in cellular and membrane regulation: an interpretive review. Am. J. Hematol., 8: 231-248, 1980.
35. Peracchia, C., Lazrak, A., and Peracchia, L. L. Molecular models of channel interaction and gating in gap junctions. In: C. Peracchia (ed.), Handbook of Membrane Channels, pp. 361-377. San Diego, CA: Academic Press, 1994.
36. Chao, S-H., Suzuki, Y., Zysk, J. R., and Cheung, W. Y. Activation of calmodulin by various metal cations as a function of ionic radius. Mol. Pharmacol., 26: 75-82, 1984.
37. Yamasaki, H. Gap junctional intercellular communication and carcinogenesis. Carcinogenesis, 11: 1051-1058, 1990.
38. Eghbali, B., Kessler, J. A., and Spray, D. C. Expression of gap junction channels in communication-incompetent cells after stable transfection with cDNA encoding connexin32. Proc. Natl Acad. Sci. USA, 87: 1328-1331, 1990.
39. Beyer, E. C., Paul, D. L., and Goodenough, D. A. Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J. Cell Biol., 105: 2621-2629, 1987.
40. Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd Ed., pp. 7.12-7.15. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1987.
41. Feigner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenze, M., Northrop, J. P., Ringold, G. M., and Danielsen, M. Lipofectin: a highly efficient lipid mediated DNA transfection procedure. Proc. Natl. Acad. Sci. USA, 84: 7413-7417, 1987.
42. src). Proc. Natl. Acad. Sci. USA, 78: 1624-1628, 1981.
43. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.), 227: 680-685, 1970.
44. Towbin, H., Staehelin, T., and Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels into nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad Sci. USA, 76: 4350-4354, 1979.
45. Safranyos, R. G., and Caveney, S. Rates of diffusion of fluorescent molecules via cell to cell membrane channels in a developing tissue. J. Cell Biol., 100: 736-747, 1985.
46. El-Fouly, M. H., Trosko, J. E., and Chang, C. C. Scrape-loading and dye transfer: a rapid and simple technique to study gap junctional inter-cellular communication. Exp. Cell Res., 168: 422-430, 1987.