Amplification of human β-actin gene by the reverse transcriptase- polymerase chain reaction: Implications for assessment of RNA from formalin- fixed, paraffin-embedded material

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 10, 1996, Pages 1205-1207

  Dakhama, Azzeddine ^a   Macek, Vasilija ^a   Hogg, James C ^a   Hegele, Richard G ^a,b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b ST PAUL S HOSPITAL   (Canada)

Author keywords

Human actin; Paraffin embedded material; Polymerase chain reaction; RNA

Indexed keywords

BETA ACTIN; FORMALDEHYDE; PARAFFIN; RNA; RNA DIRECTED DNA POLYMERASE;

ARTICLE; EMBEDDING; GENE AMPLIFICATION; HOUSEKEEPING GENE; HUMAN; HUMAN TISSUE; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SOUTHERN BLOTTING; TISSUE FIXATION;

EID: 0029842396     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.10.8813086     Document Type: Article

References (9)

1. Adolff CH, Golden JA, Kennedy PW, Goetzel EJ, Turck CW (1991) Polymerase chain reaction amplification of messages for growth factors in cells from human bronchoalveolar lavage fluids. Inflammation 15:259-268
2. Aurer I, Juhbashi T, Sekine I, Tomonaga M, Gale RP (1993) Analysis of BCR/ABL abnormalities in mRNA from 20-year-old paraffin-embedded tissue for BCR/ABL rearrangement by polymerase chain reaction. Acta Haematol 90:5-7
3. Ben-Ezra J, Johnson DA, Rossi J, Cook N, Wu A (1991) Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction. J Histochem Cytochem 39:351-354
4. de Andrés B, del Pozo V, Gallardo S, de Arruda-Chaves E, Càrdaba B, Martìn-Orozco E, Posada M, Palomino P, Lahoz C (1995) Improved method for mRNA extraction from paraffin-embedded tissues. Biotechniques 18:42-43
5. Frye RA, Benz CC, Liu E (1989) Detection of amplified oncogenes by differential polymerase chain reaction. Oncogene 4:1153-1157
6. Hodges E, Smith J (1995) Isolation of nucleic acid from paraffin embedded tissue for PCR amplification and sequencing of TcR V beta genes. Leukemia Res 19:183-186
7. Levi JE, Delcelo R, Alberti VN, Torloni H, Villa LL (1989) Human papillomavirus DNA in respiratory papillomatosis detected by in situ hybridization and the polymerase chain reaction. Am J Pathol 135:1179-1184
8. Saiki RK, Gelfand DM, Stoffel S, Scharf SJ, Higuchi RG, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491
9. Shibata D, Martin WJ, Arnheim N (1988) Analysis of DNA sequences in forty-year-old paraffin-embedded thin-tissue sections: a bridge between molecular biology and classical histology. Cancer Res 48:4564-4566