Isolation and characterization of ECT1 gene encoding CTP: Phosphoethanolamine cytidylyltransferase of Saccharomyces cerevisiae

Journal of Biochemistry

Volumn 120, Issue 5, 1996, Pages 1040-1047

  Min Seok, Rho ^a,b   Kawamata, Yuji ^a,c   Nakamura, Hidemitsu ^a   Ohta, Akinori ^a   Takagi, Masamichi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATL INST BIOSCI HUM TECHNOL   (Japan)

^c TAKEDA PHARMACEUTICAL COMPANY LIMITED   (Japan)

Author keywords

CDP ethanolamine; Kennedy pathway; Phosphatidylethanolamine; Phosphoethanolamine cytidylyltransferase; Saccharomyces cerevisiae

Indexed keywords

ETHANOLAMINE PHOSPHOTRANSFERASE; PHOSPHATIDYLETHANOLAMINE;

AMINO ACID SEQUENCE; ARTICLE; ESCHERICHIA COLI; FUNGAL GENETICS; FUNGUS MUTANT; GENE ISOLATION; NONHUMAN; OPEN READING FRAME; SACCHAROMYCES CEREVISIAE;

ESCHERICHIA COLI; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0029840156     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021497     Document Type: Article

References (39)

1. Paltauf, F., Kohlwein, S.D., and Henry, S.A. (1992) Yeast mutant with thermolabile CDP-choline synthesis: Isolation and characterization of a cholinephosphate cytidylyltransferase mutant in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression (Jones, E.W., Pringle, J.R., and Broach, J.R., eds.) pp. 415-500, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
2. Kuge, O., Nishijima, M., and Akamatsu, Y. (1991) A cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a Chinese hamster ovary cell mutant. J. Biol. Chem. 266, 6370-6376
3. Clancey, C.J., Chang, S.-C., and Dowhan, W. (1993) Cloning of a gene (PSD1) encoding phosphatidylserine decarboxylase from Saccharomyces cerevisiae by complementation of an Escherichia coli mutant. J. Biol. Chem. 268, 24580-24590
4. Trotter, P.J., Pedretti, J., Yates, E., and Voelker, D.R. (1995) Phosphatidylserine decarboxylase 2 of Saccharomyces cerevisiae: Cloning and mapping of the gene, heterologous expression, and creation of the null allele. J. Biol. Chem. 270, 6071-6080
5. Weinhold, P.A., Rounsifer, M.E., and Feldman, D.A. (1986) The purification and characterization of CTP:phosphorylcholine cytidylyltransferase from rat liver. J. Biol. Chem. 261, 5104-5110
6. Kalmer, G.B., Kay, R.J., Lachance, A., Aebersold, R., and Cornell, R.B. (1990) Cloning and expression of rat liver CTP: phosphocholine cytidylyltransferase: An amphipathic protein that controls phosphatidylcholine synthesis. Proc. Natl. Acad. Sci. USA 87, 6029-6033
7. Kent, C. (1990) Regulation of phosphatidylcholine biosynthesis. Prog. Lipid Res. 29, 87-105
8. Wang, Y., Sweitzer, T.D., Weinhold, P.A., and Kent, C. (1993) Nuclear localization of soluble CTP: phosphocholine cytidylyltransferase. J. Biol. Chem. 268, 5899-5904
9. Feldman, D.A. and Weinhold, P.A. (1993) Identification of a protein complex between choline-phosphate cytidylyltransferase and a 112-kDa protein in rat liver. J. Biol. Chem. 268, 3127-3135
10. Tsukagoshi, Y., Nikawa, J., and Yamashita, S. (1987) Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae. Eur. J. Biochem. 169, 477-486
11. Tsukagoshi, Y., Nikawa, J., Hosaka, K., and Yamashita, S. (1991) Expression in Escherichia coli of the Saccharomyces cerevisiae CCT gene encoding cholinephosphate cytidylyltransferase. J. Bacteriol. 173, 2134-2136
12. Skinner, H.B., McGee, T.P., McMaster, C.R., Bell, R.M., and Bankaitis, V.A. (1995) The Saccharomyces cerevisiae phosphatidylinositol-transfer protein effects a ligand-dependent inhibition of choline-phosphate cytidylyltransferase activity. Proc. Natl. Acad. Sci. USA 92, 112-116
13. Tijburg, L.B.M., Geelen, M.J.H., and van Golde, L.M.G. (1989) Regulation of the biosynthesis of triacylglycerol, phosphatidylcholine and phosphatidylethanolamine in the liver. Biochim. Biophys. Acta 1004, 1-19
14. Sundler, R. (1975) Ethanolaminephosphate cytidylyltransferase. Purification and characterization of the enzyme from rat liver. J. Biol. Chem. 250, 8585-8590
15. Vermeulen, P.S., Tijburg, L.B.M., Geelen, M.J.H., and van Golde, L.M.G. (1993) Immunological characterization, lipid dependence, and subcellular localization of CTP:phosphoethanolamine cytidylyltransferase purified from rat liver: Comparison with CTP: phosphocholine cytidylyltransferase. J. Biol. Chem. 268, 7458-7464
16. Nikawa, J., Yonemura, K., and Yamashita, S. (1983) Yeast mutant with thermolabile CDP-choline synthesis: Isolation and characterization of a cholinephosphate cytidylyltransferase mutant. Eur. J. Biochem. 131, 223-229
17. Tijburg, L.B.M., Vermeulen, P.S., and van Golde, L.M.G. (1992) Ethanolamine-phosphate cytidylyltransferase. Methods Enzymol. 209, 258-263
18. Hamamatsu, S., Shibuya, I., Takagi, M., and Ohta, A. (1994) Loss of phosphatidylserine synthesis results in aberrant solute sequestration and vacuolar morphology in Sacchromyces cerevisiae. FEBS Lett. 348, 33-36
19. Gerring, S.L., Connelly, C., and Mieter, P. (1991) Positional mapping of genes by chromosome blotting and chromosome fragmentation in Methods in Enzymology (Guthrie, C. and Fink, G.R., eds.) Vol. 194, pp. 57-77, Academic Press, New York
20. Ito, H., Fukuda, Y., Murata, K., and Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168
21. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
22. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467
23. Schmitt, M.E., Brown, T.A., and Trumpower, L. (1990) A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 18, 3091-3092
24. Smith, D.B. and Johnson, K.S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40
25. Feinberg, A. and Vogelstein, B. (1983) A technique for radio-labeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13
26. Hikiji, T., Miura, K., Kiyono, K., Shibuya, I., and Ohta, A. (1988) Disruption of the CHO1 gene encoding phosphatidylserine synthase in Saccharomyces cerevisiae. J. Biochem. 104, 894-900
27. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-252
28. Atkinson, K.D. (1984) Saccharomyces cerevisiae recessive suppressor that circumvents phosphatidylserine deficiency. Genetics 108, 533-543
29. Rose, M.D., Novick, P., Thomas, J.H., Botstein, D., and Fink, G.R. (1987) A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60, 237-243
30. Horowitz, D.S. and Abelson, J. (1993) A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Mol. Cell. Biol. 13, 2959-2970
31. Yoshida, Y., Sato, T., Hashimoto, T., Ichikawa, N., Nakai, S., Yoshikawa, H., Imamoto, F., and Tagawa, K. (1990) Isolation of a gene for a regulatory 15-kDa subunit of mitochondrial F1F0-ATPase and construction of mutant yeast lacking the protein. Ear. J. Biochem. 192, 49-53
32. Mauel, C., Young, M., and Karamata, D. (1991) Genes concerned with synthesis of poly(glycerol phosphate), the essential teichoic acid in Bacillus subtilis strain 168, are organized in two divergent transcription units. J. Gen. Microbiol. 137, 929-941
33. McGee, T.P., Skinner, H.B., and Bankaitis, V.A. (1994) Functional redundancy of CDP-ethanolamine and CDP-choline pathway enzymes in phospholipid biosynthesis: Ethanolamine-dependent effects on steady state membrane phospholipid composition in Saccharomyces cerevisiae. J. Bacteriol. 176, 6861-6868
34. McGee, T.P., Skinner, H.B., Whitters, E.A., Henry, S.A., and Bankaitis, V.A. (1994) A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes. J. Cell Biol. 124, 273-287
35. van Hellemond, J.J., Slot, J.W., Geelen, M.J.H., van Golde, L.M.G., and Vermeiden, P.S. (1994) Ultrastructural localization of CTP: phosphoethanolamine cytidylyltransferase in rat liver. J. Biol. Chem. 269, 15415-15418
36. Gaynor, E.G., te Heesen, S., Graham, T.R., Aebi, M., and Emr, S.D. (1994) Signal-mediated retrieval of a membrane protein from the Golgi to the ER in yeast. J. Cell Biol. 127, 653-665
37. Slonimski, P.P. and Brouillet, S. (1993) A data base of chromosome III of Saccharomyces cerevisiae. Yeast 9, 941-1029
38. Hauser, H., Pascher, I., Pearson, R.H., and Sundeil, S. (1981) Preferred conformation and molecular packing of phosphatidyl-ethanolamine and phosphatidylcholine. Biochim. Biophys. Acta 650, 21-51
39. Menon, A.K. and Stevens, V.L. (1992) Phosphatidylethanol-amine is the donor of the ethanolamine residue linking a glycosyl-phosphatidylinositol anchor to protein. J. Biol. Chem. 267, 15277-15280