Synchronization of mammalian cells by Lovastatin

Methods in Cell Science

Volumn 18, Issue 2, 1996, Pages 109-114

  Keyomarsi, Khandan ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

G1 arrest; Lovastatin; Mammalian cells; Synchronization

Indexed keywords

ENZYME INHIBITOR; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; MEVALONIC ACID; MEVINOLIN;

ARTICLE; CELL CYCLE; CELL CYCLE G1 PHASE; CELL STRAIN MCF 7; CELL SYNCHRONIZATION; CONTROLLED STUDY; CYTOTOXICITY; ENZYME INHIBITION; HUMAN; HUMAN CELL; HYPERCHOLESTEROLEMIA; TUMOR CELL;

MAMMALIA;

EID: 0029839602     PISSN: 13815741     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00122161     Document Type: Article

References (27)

1. Terasima T, Tolmach LJ (1963). Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res 30: 344-362.
2. Ashihara T, Baserga R (1979). Cell synchronization. Methods Enzymol 58: 248-262.
3. Kauffman MG, Noga SJ, Kelly TJ, Donnenberg AD (1990). Isolation of cell cycle fractions by counter-flow centrifugal elutriation. Analytical Biochem 191: 41-46.
4. Lutz MP, Gaedicke G, Hartmann W (1992). Large-scale cell separation by centrifugal elutriation. Analytical Biochem 200: 376-380.
5. Johnson RT, Downes CS, Meyn RE (1993). The synchronization of mammalian cells. In: P Fantes, R Brooks (eds), The cell cycle: A practical approach. New York: Oxford University Press, pp 1-23.
6. Rao PN, Johnson RT (1970). Mammalian cell fusion: Studies on the regulation of DNA synthesis and mitosis. Nature 225: 159-164.
7. Pedrali-Noy G, Spadari S, Miller-Faured A, Miller AO, Kruppa J, Koch G (1980). Synchronization of HeLa cell cultures by inhibition of DNA polymerase alpha with aphidicolin. Nuc Acids Res 82: 377-387.
8. D'Anna JA, Crissman HA, Jackson PJ, Tobey R (1985). Time-dependent changes in H1 content, H1 turnover, DNA elongation, and the survival of cells blocked in early S phase by hydroxyurea, aphidicolin, or 5-fluorodeoxyuridine. Biochemistry 24: 5020-5026.
9. Lambert WC, Studzinski GP (1967). Recovery from prolonged unbalanced growth induced in HeLa cells by high concentrations of thymidine. Cancer Res 27: 2364-2369.
10. Keyomarsi K, Sandoval L, Band V, Pardee AB (1991). Synchronization of tumor and normal cells from G1 to multiple cell cycles by Lovastatin. Cancer Res 51: 3602-3609.
11. Alberts AW, Chen J, Kuron G, et al. (1980). Mevinolin: A highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol- lowering agent. Proc Natl Acad Sci USA 77: 3957-3961.
12. Endo A, Kuroda M, Tansawa K (1976). Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by ML-236A and ML-236B, fungal metabolites having hypocholesterolemic activity. FEBS Lett 72: 323-326.
13. Larson RA, Chung J, Scanu AM, Yachnin S (1982). Neutrophils are required for the DNA synthetic response of human lymphocytes to mevalonic acid: Evidence suggesting that a nonsterol product of mevalonate is involved. Proc. Natl Acad Sci USA 79: 3028-3032.
14. Habenicht AJR, Glomset JA, Ross R (1980). Relation of cholesterol and mevalonic acid to the cell cycle in smooth muscle and Swiss 3T3 cells stimulated to divide by platelet-derived growth factor. J Biol Chem 255: 5134-5140.
15. Maltese WA, Sheridan KM (1987). Isoprenylated proteins in cultured cells: Subcellular distribution and changes related to altered morphology and growth arrest induced by mevalonate deprivation. J Cell Physiol 133: 471-481.
16. Langan TJ, Volpe JJ (1987). Cell cycle-specific requirement for mevalonate, but not for cholesterol, for DNA synthesis in glial primary cultures. J Neurochem 49: 513-521.
17. Doyle JW, Kandutsch AA (1988). Requirement for mevalonate in cycling cells: Quantitative and temporal aspects. J Cell Physiol 137: 133-140.
18. Sinensky M, Logel J (1985). Defective macromolecule biosynthesis and cell-cycle progression in a mammalian cell starved for mevalonate. Proc Natl Acad Sci USA 82: 3257-3261.
19. Harrock DL (1977). The H number concept. Beckman Technical Bulletin, No. 1095-NUC-77-IT.
20. Borner MM, Myers CE, Sartor O, Sei Y, Toko T, Trepel JB, Schneider R (1995). Drug-induced apoptosis is not necessarily dependent on macromolecular synthesis or proliferatin in the p53-negative human prostate cancer cell line PC-3. Cancer Res 55: 2122-2128.
21. Baldin V, Ducommun B (1995). Subcellular localisation of human weel kinase is regulated during the cell cycle. J Cell Science 108: 2425-2432.
22. Parker LL, Piwnica-Worms H (1992). Inactivation of the p34cdc2-cyclin B complex by the Human Weel tyrosine kinase. Science 257: 1955-1957.
23. Shan B, Cahng C-Y, Jones D, Lee W-H (1994). The transcription factor E2F-1 mediates the autoregulation of RB gene expression. Mol Cell Biol 14: 299-309.
24. Zhu X, Mancini MA, Cahng K-H, et al. (1995). Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression. Mol Cell Biol 15: 5017-5029.
25. Nagel W, Vallee BL (1995). Cell cycle regulation of metallothionein in human colonic cancer cells. Proc Natl Acad Sci 92: 579-583.
26. Gudas JM, Oka M, Diella F, Trepel J, Cowan KH (1994). Expression of wild-type p53 during the cell cycle in normal human mammary epithelial cells. Cell Growth Diff 5: 295-304.
27. Coppock DL, Kopman C, Scandalis S, Gilleran S (1993). Preferential gene expression in quiescent human lung fibroblasts. Cell Growth Diff 4: 483-493.