Centrifugation of bovine oocytes for nuclear micromanipulation and sperm microinjection

Human Reproduction

Volumn 11, Issue 7, 1996, Pages 1499-1503

  Tatham, B G ^a   Sathananthan, A H ^a,b   Dharmawardena, V ^b   Munesinghe, D Y ^a   Lewis, I ^a   Trounson, A O ^a  

^a MONASH MEDICAL CENTRE   (Australia)

^b LA TROBE UNIVERSITY   (Australia)

Author keywords

Bovine; Centrifugation; Embryo; Oocyte; Ultrastructure

Indexed keywords

LIPID;

ANIMAL CELL; ARTICLE; CATTLE; CELL GRANULE; CELL NUCLEUS; CELL ORGANELLE; CELL VACUOLE; CENTRIFUGATION; CHROMATIN; FEMALE; GERMINAL VESICLE; GOLGI COMPLEX; MALE; METAPHASE; MICROINJECTION; MICROSCOPY; MITOCHONDRION; MITOSIS SPINDLE; NONHUMAN; OOCYTE; SMOOTH ENDOPLASMIC RETICULUM; SPERM; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALIA; BOS TAURUS; BOVINAE;

EID: 0029839076     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.humrep.a019425     Document Type: Article

References (16)

1. Brinster, R.L., Chen, H.Y., Trumbauer, M.E. et al. (1986) Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl Acad. Sci. USA, 82, 4438.
2. Cran, D.G. (1987) The distribution of organelles in mammalian oocytes following centrifugation prior to injection of foreign DNA. Gam. Res., 18, 67-76.
3. Hammer, R.E., Pursei, V.G., Rexroad, C.E. et al. (1985) Production of transgenic rabbits, sheep and pigs by microinjection. Nature, 315, 680-683.
4. Hytell, P. (1988) Oocyte Maturation and Fertilization in Cattle - Ultrastructural Aspects. Bondegaard Tryk A/S, Herlev, Denmark.
5. Mohr, L.R. and Trounson, A.O. (1981) Structural changes associated with freezing of bovine embryos. Biol. Reprod., 25, 1009-1025.
6. Mulnard, J. and Puissant, F. (1986) Effects of ultracentrifugation on primary and secondary mouse oocytes. An ultrastructural study. Arch. Biol., 97, 301-315.
7. Nagashima, H., Kashiwazaki, N., Ashman, R. et al. (1994) Recent advances in cryopreservation of porcine embryos. Theriogenology, 41, 113-118.
8. Nakamura, K., Tsunoda, Y., Nagai, T. and Sugie, T. (1986) Effect of centrifugation of mouse eggs and their development in vitro and in vivo. Gam. Res., 15, 83-86.
9. Pangestu, M., Lewis, I. and Shaw, J. (1995) Delipidated pronuclear bovine embryos develop to blastocysts which survive cryopreservation. Proc. Fertil. Soc. Aust., 14, 116.
10. Sathananthan, A.H. (1993) Ultrastructure of fertilization and embryo development. In Trounson, A. and Gardner, D.K. (eds). Handbook of In-vitro Fertilization. CRC Press, FL, USA, pp. 237-261.
11. Sathananthan, A.H., Kirby, C., Trounson, A. et al. (1992) The effects of cooling mouse oocytes. J. Assist. Reprod. Genet., 9, 139-148.
12. Sathananthan, A.H., Tatham, B., Dharmawardena, V. et al. (1996) Inheritance of sperm centrioles/centrosomes in bovine embryos. Arch. Androl., (in press).
13. Tatham, B.G., Dowsing, A.T. and Trounson, A.O. (1995a) Enucleation by centrifugation of in vitro-matured bovine oocytes for use in nuclear transfer. Biol. Reprod., 53, 1088-1094.
14. Tatham, B.G., Pushett, D.A., Gilliam, K.J. et al. (1995b) Electrofusion of in vitro produced bovine embryonic cells for the production of isofusion contours for cells used in nuclear transfer. J. Reprod. Fertil. Suppl., 49, 549-553.
15. Wall, R.J. and Hawk, H.W. (1988) Development of centrifuged cow zygotes cultured in rabbit oviducts. J. Reprod. Fertil., 82, 673-680.
16. Wall, R.J., Pursei, V.G., Hammer, R.E. and Brinster, R.L. (1985) Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei. Biol. Reprod., 32, 645-651.