Localization of erythropoietin gene expression in proximal renal tubular cells detected by digoxigenin-labelled oligonucleotide probes

Journal of Pathology

Volumn 179, Issue 3, 1996, Pages 283-287

  Shanks, Jonathan H ^a,c   Hill, Claire M ^a   Lappin, Terence R J ^a   Maxwell, A Peter ^b  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^b BELFAST CITY HOSPITAL   (United Kingdom)

^c ROYAL PRESTON HOSPITAL   (United Kingdom)

Author keywords

digoxigenin; erythropoietin; gene expression; hypoxia; in situ hybridization; renal tubular cell

Indexed keywords

ERYTHROPOIETIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ERYTHROPOIESIS; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE LOCATION; HYPOXIA; IN SITU HYBRIDIZATION; KIDNEY TUBULE CELL; MOUSE; NONHUMAN; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL;

ANIMALS; ANOXIA; BASE SEQUENCE; DIGOXIGENIN; ERYTHROPOIETIN; GENE EXPRESSION; KIDNEY TUBULES, PROXIMAL; MALE; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; RNA, MESSENGER;

EID: 0029838888     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9896(199607)179:3<283::AID-PATH594>3.0.CO;2-P     Document Type: Article

References (22)

1. Lacombe C, Da Silva J-L, Bruneval P, et al. Peritubular cells are the site of erythropoietin synthesis in the murine kidney. J Clin Invest 1988; 81: 620-623.
2. Koury ST, Bondurant MC, Koury MJ. Localization of erythropoietin synthesizing cells in murine kidney by in situ hybridization Blood 1988; 71: 524-527.
3. Kurtz A, Eckardt K-U, Neumann R, Kaissling B, Le Hir M, Bauer C. Site of erythropoietin formation. In: Baldamus CA, Scigalla P, Wieczorek L, Koch KM, eds. Erythropoietin: From Molecular Structure to Clinical Application. Contributions to Nephrology. Basel: Karger, 1989; 76: 14-23.
4. Bachman S, Le Hir M, Eckhardt K-U. Co-localisation of erythropoietin mRNA and ecto-5′-nucleosidase immunoreactivity in peritubular cells of rat renal cortex indicates that fibroblasts produce erythropoietin. J Histochem Cytochem 1993; 41: 335-341.
5. Darby IA, Evans BA, Fu P, Lim GB, Moritz KM, Wintour EM. Erythropoietin gene expression in fetal and adult sheep kidney. Br J Haematol 1995; 89: 266-270.
6. Smith DH, Maples PB, Beru N, Goldwasser E. Identification of erythropoietin-producing cells in mammalian kidney by in situ hybridization. Blood 1986; 68: 170A.
7. Rich IN, Noé G, Vogt C, Lemke C. Erythropoietin is produced in the tubules of the renal cortex (Abstract). Blood 1990; 1(Suppl): 76.
8. Maxwell AP, Lappin TRJ, Johnston CF, Bridges JM, McGeown MG. Erythropoietin production in kidney tubular cells. Br J Haematol 1990; 74: 535-539.
9. Verpooten GF, Nuyts GD, Hoylaerts MF, et al. Immunoassay in urine of a specific marker for proximal tubular S3 segment. Clin Chem 1992; 38: 642-647.
10. Farquharson M, Harvie R, Kennedy A, McNicol AM. Detection of mRNA by in situ hybridisation and in Northern blot analysis using oligonucleotide probes labelled with alkaline phosphatase. J Clin Pathol 1992; 45:999-1002.
11. Da Silva J-L, Lacombe C, Bruneval P, et al. Tumour cells are the site of erythropoietin synthesis in human renal cancers associated with polycythemia. Blood 1990; 75: 577-582.
12. Koury ST, Bondurant MC, Koury MJ, Semenza GL. Localization of cells producing erythropoietin in murine liver by in situ hybridization. Blood 1991; 77: 2497-2503.
13. Maxwell PH, Ferguson DJP, Osmond MK, et al. Expression of a homologously recombined erythropoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. Blood 1994; 84: 1823-1830.
14. Schuster SJ, Koury ST, Bohrer M, Salceda S, Caro J. Cellular sites of extrarenal and renal erythropoietin production in anaemic rats. Br J Haematol 1992; 81: 153-159.
15. Brouwer A, Wisse E, Knook DL. Sinusoidal endothelial cells and perisinusoidal fat-storing cells. In: Arias IM, Jakoby WB, Popper H, Schacter D, Shafritz DA, eds. The Liver: Biology and Pathobiology. 2nd edn. New York: Raven Press, 1988; 665-682.
16. Eckhardt K-U, Koury ST, Tan CC, et al. Distribution of erythropoietin producing cells in rat kidneys during hypoxic hypoxia. Kidney Int 1993; 43: 815-823.
17. Jaenisch R. Transgenic animals. Science 1988; 240: 1468-1474.
18. Lo CW. Localization of low abundance DNA sequences in tissue sections by in situ hybridisation. J Cell Sci 1989; 81: 143-162.
19. Fleming KA, Evans M, Ryley C, Franklin D, Lovell-Badge RH, Morey AL. Optimization of non-isotopic in situ hybridization on formalin-fixed paraffin-embedded material using digoxigenin-labelled probes and transgenic tissues. J Pathol 1992; 167: 9-17.
20. Porter DL, Goldberg MA. Regulation of erythropoietin production. Exp Hematol 1993; 21: 399-404.
21. Loya F, Yang Y, Lin H, Goldwasser E, Albitar M. Transgenic mice carrying the erythropoietin gene promoter linked to lac Z express the reporter in proximal convoluted tubule cells after hypoxia. Blood 1994; 84: 1831-1836.
22. Maxwell PH, Osmond MK, Pugh CW, et al. Identification of the renal erythropoietin-producing cells using transgenic mice. Kidney Int 1993; 44: 1149-1162.