Substitutions of Ser for Asn-163 and Pro for Leu-264 are important for stabilization of lipase from Pseudomonas aeruginosa

Journal of Biochemistry

Volumn 120, Issue 5, 1996, Pages 915-921

  Shinkai, Akeo ^a   Hirano, Akiko ^a   Aisaka, Kazuo ^a  

^a KYOWA HAKKO KOGYO CO LTD   (Japan)

Author keywords

Lipase; pH stabilization; Pseudomonas aeruginosa; Random mutagenesis; Thermal stabilization

Indexed keywords

ASPARTIC ACID; BACTERIAL ENZYME; LEUCINE; MUTANT PROTEIN; PROLINE; SERINE; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENTROPY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN TERTIARY STRUCTURE; PSEUDOMONAS AERUGINOSA; STRUCTURE ACTIVITY RELATION; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); FELIS CATUS; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0029838225     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021506     Document Type: Article

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