Solid-phase metal chelate assay for quantifying total protein: Resistance to chemical interference

BioTechniques

Volumn 21, Issue 5, 1996, Pages 888-897

  Lim, Mark J ^a   Patton, Wayne F ^a   Shojaee, Negin ^a   Shepro, David ^a  

^a BOSTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; COLLOIDAL GOLD; FERROUS ION; FERROZINE; GELATIN; HEMOGLOBIN; METAL CHELATE; OVALBUMIN;

ARTICLE; CONTROLLED STUDY; DENSITOMETRY; IMMUNOBLOTTING; INTERMETHOD COMPARISON; NONHUMAN; PROTEIN DETERMINATION; QUANTITATIVE ASSAY; REPRODUCIBILITY; STAINING;

BOVINAE;

EID: 0029837473     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215rr01     Document Type: Article

References (27)

1. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
2. Clark, S. 1984. Determination of membrane protein concentration, p. 149-161. In J. Venter and L. Harrison (Eds.), Receptor Purification Procedures. Alan R. Liss, New York.
3. Donovan, R., C. Robison and B. Glick. 1994. Detection enhancement of computer images of bands from Western blots using a grayscale scanner. BioTechniques 17:660-661.
4. Esen, A. 1978. A simple method for quantitative, semiquantitative, and qualitative assay of protein. Anal. Biochem. 89:264-273.
5. Fazekas de St. Groth, S., R. Webster and A. Datyner. 1963. TWo new staining procedures for quantitative estimation of proteins on electrophoretic strips. Biochim. Biophys. Acta 71:377-391.
6. Gates, R. 1991. Elimination of interfering substances in the presence of detergent in the bicinchoninic acid protein assay. Anal. Biochem. 196:290-295.
7. Ghosh, S., S. Gepstein, J. Heikkila and E. Dumbroff. 1988. Use of a scanning densitometer or an ELISA plate reader for measurement of nanogram amounts of protein in crude extracts from biological tissues. Anal. Biochem. 169:227-233.
8. Gracia, E. and F. Fernandez-Belda. 1992. Ponceau S as a dye for quantitative protein assay. Its use in the presence of Triton X-100. Biochem. Int. 27:725-733.
9. Guttenberger, M., V. Neuhoff and R. Hampp. 1991. A dot-blot assay for quantitation of nanogram amounts of protein in the presence of carrier ampholytes and other possibly interfering substances. Anal. Biochem. 196:99-103.
10. Horisberger, M. and J. Rosset. 1977. Colloidal gold, a useful marker for transmission and scanning electron microscopy. J. Histochem. Cytochem. 25:295-305.
11. Hunter, J. and S. Hunter. 1987. Quantification of proteins in the low nanogram range by staining with the colloidal gold stain AuroDye. Anal. Biochem. 164:430-433.
12. Li, K., P. Geraerts, R. van Elk and J. Joose. 1989. Quantification of proteins in the subnanogram and nanogram range: comparison of the AuroDye, FerriDye, and India ink staining methods. Anal. Biochem. 182:44-47.
13. Minamide, L. and J. Hamburg. 1990. A filter paper dye-binding assay for quantitative determination of protein without interference from reducing agents or detergents. Anal. Biochem. 190:66-70.
14. Murthy, M. and T. Leroux. 1975. Determination of protein in extracts containing interfering substances and in radioactive samples following scintillation counting. Anal. Biochem. 64:18-29.
15. Nakamura, K., T. Tanaka, A. Kuwahara and K. Takeo. 1985. Microassay for proteins on nitrocellulose filter using protein dye-staining procedure. Anal. Biochem. 148:311-319.
16. Orsonneau, J., P. Douet, C. Massoubre, P. Lustenberger and S. Bernard. 1989. An improved pyrogallol red-molybdate method for determining total urinary protein. Clin. Chem. 35:2233-2236.
17. Patton, W. 1995. Biologist's perspective on analytical imaging systems as applied to protein gel electrophoresis. J. Chrom. A, 698:55-87.
18. Patton, W., L. Lam, Q. Su, M. Lui, H. Erdjument-Bromage and P. Tempst. 1994. Metal chelates as reversible stains for detection of electroblotted proteins: application to protein microsequencing and immunoblotting. Anal. Biochem. 220:324-335.
19. Patton, W. and P. Tempst. 1993. Enhancing spot detection and reducing noise from digitized electrophoretic gel images using area processing filters. Electrophoresis 14:650-658.
20. Rodriguez, L., D. Gersten, L. Ramagli and D. Johnston. 1993. Towards stoichiometric silver staining of proteins resolved in complex two-dimensional electrophoresis gels: real-time analysis of pattern development. Electrophoresis 14:628-637.
21. Said-Fernandez, S., M. Gonzalez-Garza, B. Mata-Cardenas and L. Navarro-Marmolejo. 1990. A multipurpose solid-phase method for protein determination with Coomassie brilliant blue G-250. Anal. Biochem. 191:119-126.
22. Sheffield, J., D. Graff and H. Li. 1987. A solid-phase method for the quantitation of protein in the presence of sodium dodecyl sulfate and other interfering substances. Anal. Biochem. 166:49-54.
23. Smith, P., R. Krohn, G. Hermanson, A. Mallia, F. Gartner, M. Provenzano, E. Fujimoto, N. Goeke, B. Olson and D. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
24. Sportsman, R. and J. Elder. 1984. A micro-analytical protein assay using laser densitometry. Anal. Biochem. 139:298-300.
25. Tal, M., A. Silberstein and E. Nusser. 1985. Why does Coomassie Brilliant Blue R interact differently with different proteins? A partial answer. J. Biol. Chem. 260:9976-9980.
26. Watanabe, N., S. Kamei, A. Ohkubo, M. Yamamanaka, S. Ohsawa, K. Makino and K. Tokuda. 1986. Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer. Clin. Chem. 32:1551-1554.
27. Wirth, P. and A. Romano. 1995. Staining methods in gel electrophoresis, including the use of multiple detection methods. J. Chromatogr. 698:123-143.