Functional and morphological characterization of immunomagnetically selected CD34+ hematopoietic progenitor cells

Stem Cells

Volumn 14, Issue 4, 1996, Pages 430-438

  Servida, Federica ^a   Soligo, Davide ^a   Caneva, Lorenza ^a   Bertolini, Francesco ^b   De Harven, Etienne ^a   Campiglio, Simona ^a   Corsini, Chiara ^a   Deliliers, Giorgio Lambertenghi ^a  

^a FONDAZIONE IRCCS CA GRANDA OSPEDALE MAGGIORE POLICLINICO   (Italy)

^b IRCCS   (Italy)

Author keywords

CD34 antigen; Cell separation; Ex vivo expansion; Growth factors; Hematopoietic stem cells; Long term culture initiating cells; Ultrastructure

Indexed keywords

ARTICLE; CELL ISOLATION; CELL SEPARATION; CELL SUBPOPULATION; CLONOGENIC ASSAY; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; MAGNETISM; TRANSMISSION ELECTRON MICROSCOPY;

EID: 0029837430     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.140430     Document Type: Article

References (23)

1. Andrews RG, Singer JW, Bernstein ID. Monoclonal antibody 12-8 recognizes a 115 KD molecule present on unipotent and multipotent hematopoietic colony-forming cells and their precursors. Blood 1986;67:842-845.
2. Tindle RW, Nichols RAB, Chan L et al. A novel monoclonal antibody BI-3C5 recognizes myeloblasts and non-B non-T lymphoblasts in acute leukaemias and CGL blast crises, and reacts with immature cells in normal bone marrow. Leuk Res 1985;1:417-426.
3. Civin CI, Strauss LC, Brovall C et al. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol 1984;133:157-162.
4. + marrow cells engraft lethally irradiated baboons. J Clin Invest 1988;81:951-955.
5. Lebkowski JS, Schain LR, Okrongly D et al. Rapid isolation of human CD34 hematopoietic stem cells-purging of human tumour cells. Transplantation 1992;53:1011-1019.
6. + selected cells in clinical transplantation. STEM CELLS 1993;12:577-585.
7. Miltenyi S, Muller W, Radbruch A. High gradient magnetic cell separation with MACS. Cytometry 1990;11:231-238.
8. + hematopoietic progenitor cells by high gradient magnetic cell sorting (MACS). In: Wunder E, Sovalat H, Hénon PR et al., eds. Hematopoietic Stem Cells. Dayton, OH: AlphaMed Press, 1994:201-213.
9. Graham RC, Karnovsky MJ. The early stages of absorption of injected horseradish peroxide in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 1966;14:291-302.
10. Soligo D, Servida F, Annaloro C et al. Cytokine-dependent ex-vivo expansion of clonogenic bone marrow progenitor cells for bone marrow transplantation. Bone Marrow Transplant 1994;suppl 1:94.
11. Eaves AC, Cashman JD, Gaboury LA et al. Unregulated proliferation of primitive chronic myeloid leukemia progenitors in the presence of normal marrow adherent cells. Proc Natl Acad Sci USA 1986;83:5306-5310.
12. Sutherland HJ, Lansdorp PM, Henkelman DH et al. Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers. Proc Natl Acad Sci USA 1990;87:3584-3588.
13. Taswell C. Limiting dilution assays for the determination of immunocompetent cell frequencies. J Immunol 1981;126:1614-1619.
14. + marrow cells in patients with breast cancer or neuroblastoma. Blood 1991;77:1717-1722.
15. Brugger W, Heimfeld S, Berenson RJ et al. Reconstitution of hematopoiesis after high-dose chemotherapy by autologous progenitor cells generated ex vivo. N Engl J Med 1995;333:283-287.
16. + hematopoietic progenitor cells: positive selection using a closed, automated avidin-biotin immunoadsorption system. In: Wunder E, Sovalat H, Hénon PR et al., eds. Hematopoietic Stem Cells. Dayton, OH: AlphaMed Press, 1994:231-239.
17. + cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis) using five separation systems. STEM CELLS 1995;13:524-532.
18. + human hemopoietic progenitor cells from the peripheral blood: enzyme-, carbohydrate- and immunohistochemistry, morphometry, and ultrastructure. Leuk Lymphoma 1995;16:483-491.
19. + hematopoietic stem cells from human peripheral blood by high-gradient magnetic cell sorting. Cytometry 1993;14:384-392.
20. Lill MC, Lynch M, Fraser JK et al. Production of functional myeloid cells from CD34-selected hematopoietic progenitor cells using a clinically relevant ex vivo expansion system. STEM CELLS 1994;12:626-637.
21. + cells into the myeloid lineage. Blood 1992;80:1405-1412.
22. + bone marrow progenitor cells in patients with non-Hodgkin's lymphoma. Blood 1995;85:1647-1654.
23. +-selected peripheral blood progenitor cells mobilized by recombinant granulocyte- and granulocyte-macrophage colony-stimulating factors. Blood 1995;86:2052-2054.