Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis

Journal of Clinical Pathology

Volumn 49, Issue 8, 1996, Pages 642-647

  Horner, P J ^a,b,d   Ali, M ^a   Parker, D ^c   Weber, J N ^a   Taylor Robinson, D ^b   McClure, M O ^a  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

^b MRC Sexually Transmitted Dis R   (United Kingdom)

^c Murex Diagnostics Ltd   (United Kingdom)

^d BRISTOL ROYAL INFIRMARY   (United Kingdom)

Author keywords

Antibody; Chlamydia trachomatis; ELISA; Heat shock protein

Indexed keywords

ANTIBODY; BETA GALACTOSIDASE; HEAT SHOCK PROTEIN;

ARTICLE; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE;

EID: 0029835225     PISSN: 00219746     EISSN: None     Source Type: Journal    
DOI: 10.1136/jcp.49.8.642     Document Type: Article

References (15)

1. Hutchinson GR, Taylor-Robinson D, Dourmashkin RR. Growth and effect of chlamydiae in human bovine oviduct organ cultures. Br J Ven Dis 1970;55:194-202.
2. Watkins NG, Hadlow WJ, Moos AB, Caldwell HD. Ocular delayed hypersensitivity: A pathogenic mechanism of chlamydial conjunctivitis in guinea pigs. Proc Natl Acad Sci USA 1986;83:7480-4.
3. Taylor HR, Johnson SL, Schachter J, Caldwell HD, Prendergast RA. Pathogenesis of trachoma: The stimulus for inflammation. J Immunol 1987;138:3023-7.
4. Morrison RP, Belland RJ, Lyng K, Caldwell HD. Chlamydial disease pathogenesis: The 57-kD chlamydial hypersensitivity antigen is a stress response protein. J Exp Med 1989;170:1271-83.
5. Morrison RP, Su H, Lyng K, Yuan Y. The Chlamydia trachomatis operon is homologous to the groE stress response operon of Escherichia coli. Infect Immun 1990;58:2701-5.
6. Cerrone MC, Ma JJ, Stephens RS. Cloning and sequence of the gene for the heat shock protein 60 from Chlamydia trachomatis and immunological reactivity of the protein. Infect Immun 1991;59:79-90.
7. Bavoil P, Stephens RS, Falkow S. A soluble 60 kiloDalton antigen of Chlamydia spp. is a homologue of Escherichia coli Groel. Mol Microbiol 1990;4:461-9.
8. Craig EA. Chaperones: helpers along the pathways to protein folding. Science 1993;260:1902-4.
9. Wagar EA, Schachter J, Bavoil P, Stephens RS. Differential human serological response to two 60,000 molecular weight Chlamydia trachomatis antigens. J Infect Dis 1990; 162:922-7.
10. Toye B, Laferriere C, Claman P, Jessamine P, Peeling R. Association between antibody to the chlamydial heat-shock protein and tubal infertility. J Infect Dis 1993; 168:1236-40.
11. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989.
12. Towbin J, Staehlin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
13. Bjerrum OJ, Schafer-Nielsen C Buffer systems and transfer parameters for semi-dry electroblotting with a horizontal apparatus. In: Dunn MJ ed. Electrophoresis '86. Weinheim, Germany: VCH Verlag, 1986:315-27.
14. Thomas BJ, Reeve P, Oriel JD. Simplified serological test for antibodies to Chlamydia trachomatis. J Clin Microbiol 1976;4:6-10.
15. Jackett PS, Bothamley GH, Batra HV, Mistry A, Young DB, Ivanyi J. Specificity of antibodies to immunodominant mycobacterial antigens in pulmonary tuberculosis. J Clin Microbiol 1988;26:2313-8.