Transcriptional regulation of the human cholesterol 7α-hydroxylase gene (CYP7A) in HepG2 cells

Journal of Lipid Research

Volumn 37, Issue 9, 1996, Pages 1831-1841

  Wang, Dan Ping ^a   Stroup, Diane ^a   Marrapodi, Maria ^a,b   Crestani, Maurizio ^a,b   Galli, Giovanni ^b   Chiang, John Y L ^a  

^a NORTHEAST OHIO MEDICAL UNIVERSITY   (United States)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

cytochrome P450; gene transcription; glucocorticoid; hepatocyte nuclear factor; HNF 3 ; hormonal regulation; insulin; nuclear receptors; phorbol esters; thyroid hormones

Indexed keywords

CHOLESTEROL 7ALPHA MONOOXYGENASE; DEXAMETHASONE; GLUCOCORTICOID RECEPTOR; INSULIN; LUCIFERASE; NUCLEAR FACTOR; PHORBOL 13 ACETATE 12 MYRISTATE; THYROID HORMONE RECEPTOR; THYROXINE; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; GENE REPRESSION; HEPATOMA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; TRANSCRIPTION REGULATION;

BASE SEQUENCE; BINDING SITES; CELL LINE; CHOLESTEROL 7-ALPHA-HYDROXYLASE; CONSENSUS SEQUENCE; DNA-BINDING PROTEINS; ENZYME REPRESSION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, REPORTER; GLUCOCORTICOIDS; HEPATOCYTE NUCLEAR FACTOR 3-ALPHA; HUMANS; INSULIN; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PHORBOL ESTERS; PROMOTER REGIONS (GENETICS); RECEPTORS, GLUCOCORTICOID; RECOMBINANT FUSION PROTEINS; THYROID HORMONES; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 0029834337     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Vlahcevic, Z. R., D. M. Heuman, and P. B. Hylemon. 1991. Regulation of bile acid synthesis. Hepatology. 13: 590-600.
2. Crestani, M., W. G. Karam, and J. Y. L. Chiang. 1994. Effects of bile acids and steroid/thyroid hormones on the expression of cholesterol 7α-hydroxylase mRNA and the CYP7 gene in HepG2 cells. Biochem. Biophys. Res. Commun. 198: 546-553.
3. Pandak, W. M., R. T. Stravitz, V. Lucas, D. M. Heuman, and J. Y. L. Chiang. 1995. HepG2 cells: a model for studies on the regulation of human cholesterol 7α-hydroxylase at the molecular level. Am. J. Physiol. 270: G401-410.
4. Hylemon, P. B., E. C. Gurley, R. T. Stravitz, J. S. Litz, W. M. Pandak, J. Y. L. Chiang, and Z. R. Vlahcevic. 1992. Hormonal regulation of cholesterol 7α-hydroxylase mRNA levels and transcriptional activity in primary rat hepatocyte cultures. J. Biol. Chem. 267: 16866-16871.
5. Princen, H. M. G., P. Meijer, and B. Hofstee. 1989. Dexamethasone regulates bile acid synthesis in monolayer cultures of rat hepatocytes by induction of cholesterol 7α-hydroxylase. Biochem. J. 262: 341-348.
6. Molowa, D. T., W. S. Chen, G. M. Cimis, and C. P. Tan. 1992. Transcriptional regulation of the human cholesterol 7α-hydroxylase gene. Biochemistry. 31: 2539-2544.
7. Lavery, D. J., and U. Schibler. 1993. Circadian transcription of the cholesterol 7α-hydroxylase gene may involve the liver-enriched bZIP protein DBP. Genes Dev. 7: 1871-1884.
8. Lee, Y-H., J. A. Alberta, F.J. Gonzalez, and D.J. Waxman. 1994. Multiple functional DBP sites on the promoter of the cholesterol 7α-hydroxylase P450 gene, CYP7. J. Biol. Chem. 269: 14681-14689.
9. Rudel, L., C. Deckelman, M. Wilson, M. Scobey, and R. Anderson. 1994. Dietary cholesterol and down-regulation of cholesterol 7α-hydroxylase and cholesterol absorption in African green monkeys. J. Clin. Invest. 93: 2463-2472.
10. Poorman, J. A., R. A. Buck, S. A. Smith, M. L. Overturf, and D. S. Loose-Mitchell. 1993. Bile acid excretion and cholesterol 7α-hydroxylase expression in hypercholesterolemia-resistant rabbits. J. Lipid Res. 34: 1675-1685.
11. Crestani, M., G. Galli, and J. Y. L. Chiang. 1993. Genomic cloning, sequencing and analysis of hamster cholesterol 7α-hydroxylase gene (CYP7). Arch. Biochem. Biophys. 306: 451-460.
12. Bertolotti, M., N. Abate, P. Loria, M. Dilengite, F. Carubbi, A. Pinetti, A. Digrisolo, and N. Carulli. 1991. Regulation of bile acid synthesis in humans: effect of treatment with bile acids, cholestyramine, or simvastatin on cholesterol 7α-hydroxylation rates in vivo. Hepatology. 14: 830-837.
13. Karam, W. G., and J. Y. L. Chiang. 1994. Expression and purification of human cholesterol 7α-hydroxylase in Escherichia coli. J. Lipid Res. 35: 1222-1231.
14. Cooper, A. D., W. Y. Craig, T. Taniguchi, and G. T. Everson. 1994. Characteristics and regulation of bile salts synthesis and secretion by human hepatoma HepG2 cells. Hepatology. 20: 1522-1531.
15. Taniguchi, T., J. Chen, and A. D. Cooper. 1994. Regulation of cholesterol 7α-hydroxylase gene expression in HepG2 cells. J. Biol. Chem. 269: 10071-10078.
16. Crestani, M., D. Stroup, and J. Y. L. Chiang. 1995. Hormonal regulation of the cholesterol 7α-hydroxylase gene (CYP7). J. Lipid Res. 36: 2419-2432.
17. Chiang, J. Y. L., and D. Stroup. 1994. Identification of a liver nuclear protein factor responsive to bile acid inhibition of cholesterol 7α-hydroxylase gene. J. Biol. Chem. 269: 17502-17507.
18. Ramirez, M. I., D. Karaoglu, D. Haro, C. Barillas, R. Bashirzadeh, and G. Gil. 1994. Cholesterol and bile acid regulate cholesterol 7α-hydroxylase expression at the transcriptional level in culture and in transgenic mice. mol. Cell. Biol. 14: 2809-2821.
19. Hoekman, M. F. M., J. M. J. Rientjes, J. Twisk, R. J. Planta, H. M. G. Princen, and W. H. Mager. 1993. Transcriptional regulation of the gene encoding cholesterol 7α-hydroxylase in the rat. Gene. 130: 217-223.
20. Wang, D. P., and J. Y. L. Chiang. 1994. Structure and nucleotide sequences of the human cholesterol 7α-hydroxylase gene (CYP7). Genomics. 20: 320-323.
21. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. A. Smith, J. G. Seidman, and K. Struhl. 1987. Chapter 9. Introduction of DNA into mammalian cells: stable transfer of genes into mammalian cells. In Current Protocols in Molecular Biology. Wiley Interscience, N.Y. 9.5.1-6.
22. De Wet, J., K. V. Wood, M. DeLuca, D. R. Helinski, and S. Subramani. 1987. Firefly luciferase gene: structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737.
23. Brinster, R. L., T. Choi, M. Hung, C. Gorman, and R. Jaenisch. 1991. A gene intron increases gene expression in transgenic mice. Mol. Cell. Biol. 11: 3070-3074.
24. Zannis, V. I., P. Cardot, C. Cladaras, E. E. Zannis, J. Chambaz, I. Talianidis, and D. Kardassis. 1994. Transcriptional regulation of the human apolipoprotein genes. In Liver Gene Expression. F. Tronche and M. Yaniv, editors. R. G. Landes Co., Austin. 63-85.
25. Diamond, M. I., J. N. Miner, S. K. Yoshinaga, and K. R. Yamamoto. 1990. Transcriptional factor interactions: selectors of positive or negative regulation from a single DNA element. Science. 249: 1266-1272.
26. Costa, R. H. 1994. Hepatocyte nuclear factors 3/Fork-head protein family: mammalian transcription factors that possess divergent cellular expression patterns and binding specificities in liver gene. In Liver Gene Expression. F. Tronche and M. Yaniv, editors. R. G. Landes Co., Austin. 183-205.
27. Miner, J. N., and K. R. Yamamoto. 1991. Regulatory crosstalk at composite response elements. Trends Bio. Sci. 16:423-426.
28. Ness, G. C., L. C. Pendleton, Y. C. Li, and J. Y. L. Chiang. 1990. Effect of thyroid hormone on hepatic cholesterol 7α-hydroxylase, LDL receptor, HMG-CoA reductase, farnesyl pyrophosphate synthetase and apolipoprotein A-I mRNA levels in hypophysectomized rats. Biochem. Biophys. Res. Commun. 172: 1150-1156.
29. Ness, G. C., L. C. Pendleton, and Z. Zhao. 1994. Thyroid hormone rapidly increases cholesterol 7α-hydroxylase mRNA levels in hypophysectomized rats. Biochim. Biophys. Acta. 1214: 229-233.
30. Baniahmad, A., C. Steiner, A. C. Kohne, and R. Renkawitz. 1990. Modular structure of a chicken lysozyme silencer: involvement of an unusual thyroid hormone receptor binding site. Cell. 61: 505-514.
31. Nawaz, Z., M-J. Tsai, and B. W. O'Malley. 1995. Specific mutations in the ligand binding domain selectively abolish the silencing function of human thyroid hormone receptor β. Proc. Natl. Acad. Sci. USA. 92: 11691-11695.
32. Baniahmad, A., I. Ha, D. Reinberg, S. Tsai, M-J. Tsai, and B. W. O'Malley. 1993. Interaction of human thyroid hormone receptor β with transcription factor TFIIB may mediate target gene derepression and activation by thyroid hormone. Proc. Natl. Acad. Sci. USA. 90: 8832-8836.
33. Kurokawa, R., J. DiRenzo, M. Boehm, J. Sugarman, B. Glass, M. G. Rosenfeld, R. A. Heyman, and C. K. Glass. 1994. Regulation of retinoid signalling by receptor polarity and allosteric control of ligand binding. Nature. 371: 528-531.
34. Huang, X. P., X. T. Fan, J. F. Desjeux, and M. Castagna. 1992. Bile acids, non-phorbol ester type tumor promoters, stimulate the phosphorylation of protein kinase C substrate in human platelets and colon cell line HT29. Int. J. Cancer. 52: 444-450.
35. Hirano, F., H. Tanaka, Y. Makino, K. Okamoto, M. Inaba, T. Miura, and I. Makino. 1995. Regulation of the major histocompatibility complex class mRNA expression by bile acids in cultured human hepatoma cells. Biochem. Biophys. Res. Commun. 208: 935-942.
36. Stravitz, R. T., Z. R. Vlahcevic, E. C. Gurley, and P. B. Hylemon. 1995. Repression of cholesterol 7α-hydroxylase transcription by bile acids is mediated through protein kinase C in primary cultures of rat hepatocytes. J. Lipid Res. 36: 1359-1368.
37. Subbiah, M. T. R., and R. L. Yunker. 1984. Cholesterol 7α-hydroxylase of rat liver: an insulin sensitive enzyme. Biochem. Biophys. Res. Commun. 124: 896-902.
38. Twisk, J., M. F. M. Hoekman, E. M. Lehmann, P. Meijer, W. H. Mager, and H. M. G. Princen. 1995. Insulin sup presses bile acid synthesis in cultured rat hepatocytes by down-regulation of cholesterol 7α-hydroxylase and sterol 27-hydroxylase gene transcription. Hepatology. 21: 501-510.
39. O'Brien, R. M., E. L. Noisin, A. Suwanichkul, T. Yamasaki, P. C. Lucas, I-C. Wang, D. R. Powell, and D. K. Granner. 1995. Hepatic nuclear factor 3- and hormone-regulated expression of the phosphoenolpyruvate carboxykinase and insulin-like growth factor-binding protein 1 genes. Mol. Cell. Biol. 15: 1747-1758.
40. O'Brien, R. M., P. C. Lucas, C. D. Forest, M. A. Magnuson, and D. K. Granner. 1990. Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription. Science. 249: 533-537.