Improved dicistronic mRNA expression vectors for efficient selection of transfectants highly expressing foreign genes

BioTechniques

Volumn 21, Issue 3, 1996, Pages 398-402

  Kobayashi, Masayuki ^a   Yamauchi, Yukika ^a   Tanaka, Akiko ^a   Shimamura, Seiichi ^a  

^a MORINAGA MILK INDUSTRY CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ANIMAL CELL; ARTICLE; CODON; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC TRANSFECTION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME;

ANIMALIA;

EID: 0029832240     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96213bm12     Document Type: Article

References (7)

1. Davies, M.V. and R.J. Kaufman. 1992. The sequence context of the initiation codon in the encephalomyocarditis virus leader modulates efficiency of internal translation initiation. J. Virol. 66:1924-1932.
2. Kaminski, A., M.T. Howell and R.J. Jackson. 1990. Initiation of encephalomyocarditis virus RNA translation: the authentic initiation site is not selected by a scanning mechanism. EMBO J. 9:3753-3759.
3. Kaufman, R.J., M.V. Davies, L.C. Wasley and D. Michnick. 1991. Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus. Nucleic Acids Res. 19:4485-4490.
4. Kobayashi, M., Y. Yamauchi, K. Yamaguchi and A. Tanaka. 1995. Transient expression assay for antisense RNAs using episomal replication of plasmids: effective reduction of retinoblastoma gene (Rb-1) product by its antisense RNA complementary to 3′-untranslated region. Antisense Res. Dev. 5:141-148.
5. Kriegler, M. 1990. Gene transfer, p. 1-81. In Gene Transfer and Expression: A Laboratory Manual. Stockton Press, New York.
6. MacGregor, G.R., G.P. Nolan, S. Fiering, M. Roederer and L.A. Herzenberg. 1991. Use of E. coli lac Z (β-galactosidase) as a reporter gene, p. 217-235. In E.J. Murray (Ed.), Gene Transfer and Expression Protocols. Humana Press, Clifton, New Jersey.
7. Niwa, H., K. Yamamura and J. Miyazaki. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200.