Sixteen weeks of hexarelin therapy in aged dogs: Effects on the somatotropic axis, muscle morphology, and bone metabolism

Journals of Gerontology - Series A Biological Sciences and Medical Sciences

Volumn 51, Issue 6, 1996, Pages

  Cella, Silvano G ^a   Cerri, Cesare G ^b   Daniel, Sergio ^c   Sibilia, Valeria ^a   Rigamonti, Antonello ^a   Cattaneo, Lorena ^a   Deghenghi, Romano ^d   Müller, Eugenic E ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b SAN GERARDO HOSPITAL   (Italy)

^c DEPARTMENT OF NEUROSURGERY   (Italy)

^d Ardana   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; GROWTH HORMONE; HEXARELIN; SOMATOMEDIN C;

AGED; AGING; ANIMAL TISSUE; ARTICLE; BODY COMPOSITION; CONTROLLED STUDY; DOG; GROWTH HORMONE RELEASE; HORMONE RESPONSE; MUSCLE; NONHUMAN; OSSIFICATION; OSTEOLYSIS; PRIORITY JOURNAL; SUBCUTANEOUS DRUG ADMINISTRATION;

ANIMALIA; CANIS FAMILIARIS;

EID: 0029832033     PISSN: 10795006     EISSN: None     Source Type: Journal    
DOI: 10.1093/gerona/51A.6.B439     Document Type: Article

References (65)

1. Arce, V.M.; Cella, S.G ; Loche, S.; Ghigo, E., Devesa, J., Müller, E.E. Synergistic effect of growth hormone-releasing hormone (GHRH) and clonidine in stimulating GH release in young and old dogs. Brain Res. 537:359-362; 1990.
2. Arvat, E.; Gianotti, L.; Grottoli, S Arginine and growth hormone-releasing hormone restore the blunted growth hormone-releasing activity of hexarelin in elderly subjects. J. Clin. Endocrinol. Metab. 79:1440-1443; 1994.
3. Arvat, E.; Gianotti, L.; Di Vito, L. Modulation of growth hormone-releasing activity of hexarelin in man. Neuroendocrinology 61:51-56; 1995.
4. Ayling, C.M.; Moreland, B.H.; Zanelli, J M.; Schulster, D. Human growth hormone treatment of hypophysectomized rats increases the proportion of type-1 fibers in skeletal muscle. J. Endocrinol. 123:429-435; 1989.
5. Badger, T M.; Millard, W.J ; McCormick, G.F.; Bowers, C.Y.; Martin, J.B. The effects of growth hormone (GH)-releasing peptides on GH secretion in perifused pituitary cells of adult male rats. Endocrinology 115:1432-1437;1984.
6. Bilezikjian, L.M.; Vale, W.W. Chronic exposure of cultured rat anterior pituitary cells to GRF causes partial loss of responsiveness to GRF. Endocrinology 115:2032-2034; 1984.
7. Bilezikjian, L.M.; Seifert, H.; Vale, W. Desensitization to growth hormone-releasing factor (GRF) is associated with down-regulation of GRF-binding sites. Endocrinology 118:2045-2052; 1986.
8. Black, D.; Duncan, A.; Robins, S.P. Quantitative analyses of the pyridinium crosslinks of collagen in urine using ion-paired reversed-phase high-performance liquid chromatography. Anal. Biochem. 169:197-203; 1988.
9. Blackman, M.R.; Vittone, J ; Corpas, E.; Busby-Whitehead, J.; Stevens, T.; Stewart, K.; Tobin, J.; Bellantoni, M.F.; Roth, J.; Spencer, R.G.S.; Harman, S.M. Growth hormone releasing hormone (GHRH) effects in healthy elderly men. In: Abstract book of the International Symposium on Growth Hormone Secretagogues. St. Petersburg Beach, FL: Serono Symposia, 1994:23.
10. Ceda, G.P.; Hoffman, A.R. Growth hormone-releasing factor desensitization in rat anterior pituitary cells in vitro. Endocrinology 116:1334-1340; 1985.
11. Ceda, G.P.; Valenti, G.; Butturini, V.; Hoffman, A.R. Diminished pituitary responsiveness to growth hormone-releasing factor in aging male rats. Endocrinology 118:2109-2114; 1986.
12. Ceda, G.P.; Denti, L.; Hoffman, A.R.; Ceresini, G.; Valenti, G. Aging and pituitary responses to hypothalamic peptides In: Segal, H.L., ed. Protein metabolism in aging. New York: Wiley Liss, 1990:335-344.
13. Cella, S.G ; Moiraghi, V.; Minuto, F.; Barreca, A.; Cocchi, D.; DeGennaro Colonna, V., Reina, G.; Müller, E.E. Prolonged fasting or clonidine can restore the defective growth hormone secretion in old dogs. Acta Endocrinol. 121:177-184; 1989.
14. Cella, S.G., Arce, V.M.; Pieretti, F.; Locateili, V.; Settembrini, B.P.; Muller, E.E. Combined administration of growth hormone-releasing hormone and clonidine restores defective growth hormone secretion in old dogs. Neuroendocrinology 57:432-438; 1993.
15. Cella, S.G.; Locatelli, V ; Poratelli, M.; DeGennaro Colonna, V.; Imbimbo, B.P.; Deghenghi, R., Müller, E.E Hexarelin, a potent GHRP analogue, interactions with GHRH and clonidine in young and aged dogs. Peptides 16:81-86; 1995.
16. Cocola, F.; Udeschini, G.; Panerai, A.E.; Neri, F.; Müller, E.E. A rapid radioimmunoassay method for growth hormone in dog plasma. Proc. Soc. Exp Biol. Med. 151:140-151; 1976.
17. Compston, J.E. HRT and osteoporosis. Br. Med Bull. 48:309-344; 1992.
18. Corpas, E.; Harman, S.M.; Piñeyro, M.A.; Roberson, R.; Blackman, M.R. Growth hormone (GH)-releasing hormone-(1-29) twice daily reverses the decreased GH and insulin-like growth factor-I levels in old men. J. Clin. Endocrinol. Metab. 75:530-535; 1992.
19. Cowan, J.S.; Gaul, P ; Moor, B.C.; Kraicer, J. Secretory bursts of growth hormone in the dog may be initiated by somatostatin withdrawal. Can. J. Physiol. Pharmacol 62:199-207; 1984
20. Cuneo, R.C., Salomon, F ; Wiles, C.M.; Sonksen, P H. Skeletal muscle performance in adults with growth hormone deficiency. Horm. Res. 33 (Suppl 4):55-60; 1990.
21. Daughaday, W.H.; Kapadia, M ; Mariz, I. Serum somatomedin binding proteins: physiologic significance and interference in radioligand assay. J. Lab. Clin. Med. 109:355-363; 1987.
22. Davis, J.R.E.; Sheppard, M.C.; Shakespear, R.A.; Lynch, S.S.; Clayton, R.N. Does growth hormone releasing factor desensitize the somatotroph? Interpretation of responses of growth hormone during and after 10-hour infusion of GRF 1-29 amide in man. Clin. Endocrinol. 24:135-140; 1986.
23. Deghenghi, R.; Cananzi, M.M.; Torsello, A.; Battisti, C.; Müller, E.E.; Locatelli, V. GH-releasing activity of Hexarelin, a new growth hormone releasing peptide, in infant and adult rats. Life Sci. 54:1321-1328; 1994.
24. Delmas, P.D. Biochemical markers of bone turnover. J. Bone Mineral Res. 8:S549-S555; 1993.
25. D'Ercole, A.J.; Stiles, A.D.; Underwood, L.E. Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc. Natl. Acad. Sci. USA 81:935-939; 1984.
26. Dubowitz, V., Brooke, M.H. Muscle biopsy: a modern approach. London: W.B. Saunders, 1982.
27. Eyre, D.R. Collagen crosslinking amino-acids. Methods Enzymol. 144:115-139; 1987
28. Finkelstein, J W.; Boyar, R.M.; Roffwarg, H.P.; Kream, J.; Hellman, L. Age-related changes in the twenty-four-hour secretion of growth hormone. J. Clin. Endocrinol. Metab. 35:665-670; 1972.
29. Franchimont, P.; Urban-Choffray, D.; Lambelin, P.; Fontaine, M.A.; Frangin, G.; Reginster, J.Y Effects of repetitive administration of growth hormone-releasing hormone on growth hormone secretion, insulin-like growth factor-I, and bone metabolism in postmenopausal women. Acta Endocrinol. 120:121-128; 1989.
30. Frost, H.M. Mathematical element of lamellar bone remodeling. Springfield, IL: Charles C Thomas; 1964.
31. Ghigo, E.; Arvat, E.; Rizzi, G.; Goffi, S.; Grottoli, S.; Mucci, M.; Boghen, M.F.; Camanni, F. Growth hormone-releasing activity of growth hormone-releasing peptide-6 is maintained after short-term oral pretreatment with the hexapeptide in normal aging. Eur. J. Endocrinol. 131:499-503; 1994a.
32. Ghigo, E.; Arvat, E.; Gianotti, L. Growth hormone-releasing activity of hexarelin, a new synthetic hexapeptide, after intravenous, subcutaneous, intranasal, and oral administration in man. J. Clin. Endocrinol. Metab. 78:693-698; 1994b.
33. Harris, W.H.; Heaney, R.P. Effect of growth hormone on skeletal mass in adult dogs. Nature 223:403-404; 1969.
34. Hoffman, A.R.; Pyka, G.; Lieberman, S.A.; Ceda, G.P.; Marcus, R. The somatopause. In: Müller, E.E.; Cocchi, D.; Locatelli, V., eds. Growth hormone and somatomedins during life span. Berlin: Springer-Verlag, 1993:265-274.
35. Holloway, L.; Butterfield, G.; Hintz, R.L.; Gesundheit, N.; Marcus, R. Effects of recombinant human growth hormone on metabolic indices, body composition, and bone turnover in healthy elderly women. J. Clin. Endocrinol. Metab. 79:470-479; 1994.
36. Huhn, W.C.; Hartman, M.L.; Pezzoli, S.S.; Thomer, M.O. Twenty-four-hour growth hormone (GH)-releasing peptide (CHRP) infusion enhances pulsatile GH secretion and specifically attenuates the responses to a subsequent GHRP bolus. J Clin. Endocrinol. Metab. 76:1202-1208; 1993.
37. Javorski, J.F.G.; Liskova-Kiar, M.; Uhthoff, H.K. Effect of long-term immobilization on the pattern of bone loss in older dogs. J. Bone Joint Surg. 62B:104; 1980.
38. Jovino, M.; Monteleone, P.; Steardo, L. Repetitive growth hormone-releasing hormone administration restores the attenuated growth hormone (GH) response to GH-releasing hormone in normal aging. J. Clin. Endocrinol. Metab. 69:910-913; 1989.
39. Kahler, L.W.; Gliessman, P.; Craven, J.; Critchlow, V. Loss of enhanced nocturnal growth hormone secretion in aged rhesus monkeys. Endocrinology 119:1281-1284, 1986
40. Kendall, M.G. Rank correlation methods, 2nd ed. London: Griffin, 1955.
41. Li, X.Y.; Jee, W.S.S.; Li, Y L.; Patterson-Buckendahl, P.- Transient effects of subcutaneously administered prostaglandin E2 on cancellous and cortical bone in young adult dogs. Bone 11:353-364; 1990.
42. Lieberman, S.A.; Mitchell, A.M.; Marcus, R.; Hoffman, A.R. The IGF-generation test: assessing the response to growth hormone with aging andestrogen therapy. In. Abstract book of the International Symposium on Growth Hormone II: Basic and clinical aspects. Tarpon Spring, FL: Serono Symposia, 1992:39.
43. Locatelli, V.; Grilli, R.; Torsello, A.; Cella, S.G.; Wehrenberg, W.B.; Müller, E.E. Growth hormone-releasing hexapeptide is a potent stimulator of growth hormone gene expression and release in the growth hormone-releasing hormone-deprived infant rat. Pediatr. Res. 36:169-174; 1994.
44. Momany, F.A ; Bowers, C.Y.; Reynolds, C.A.; Caang, D.; Hong, A.; Newlander, K. Design, synthesis, and biological activity of peptides which release growth hormone in vitro. Endocrinology 108:31-39; 1981.
45. Müller, E.E. Neural control of somatotropic function. Physiol. Rev. 67:962-1053, 1987.
46. Panzeri, G.; Torsello, A.; Cella, S.G.; Müller, E.E.; Locatelli, V. Age-related modulatory activity by a cholinergic agonist on the growth hormone response to GH-releasing hormone in the rat. Proc. Soc. Exp. Biol. Med. 193:301-305; 1989.
47. Rubin, C.T., Bain, S.D.; McLeod, K.J. Suppression of the osteogenic response in the aging skeleton. Calcif. Tissue Int. 50:306-313; 1992.
48. Rudman, D. Growth hormone, body composition, and aging. J. Am. Geriatr. Soc. 33:800-807; 1985.
49. Rudman, D.; Kutner, M.H.; Rogers, C.M.; Lubin, M.F.; Fleming, G.A.; Bain, R.P. Impaired growth hormone secretion in the adult population. J. Clin. Invest. 67:1361-1369; 1981.
50. Rudman, D.; Feller, A.G.; Nagraj, H S. Effects of human growth hormone in men over 60 years old. N. Engl. J. Med. 323:1-6; 1990.
51. Sartorio, A.; Conti, A.; Monzani, M.; Narici, M.; Morabito, F.; Faglia, G. Growth hormone treatment in adults with GH deficiency: effects on muscle size, strength and bone turnover. In: Andreoli, M.; Shields, M., eds. Highlights in molecular and clinical endocrinology: frontiers in endocrinology, Vol. 9. Rome: Ares Serono Symposia Publications, 1994:221-224.
52. Shibasaki, T.; Shizume, K., Nakahara, M.; Masuda, A.; Jibiki, K.; Demura, H.; Wakabayashi, I.; Ling, N. Age-related changes in plasma growth hormone response to growth hormone releasing-hormone in man. J. Clin. Endocrinol. Metab. 58:212-214; 1984.
53. Sonntag, W.E.; Steger, R.W.; Forman, L.J.; Meites, J. Decreased pulsatile release of growth hormone in old male rats. Endocrinology 107:1875-1879; 1980.
54. Sonntag, W.E.; Gough, A. Growth hormone-releasing hormone-induced release of growth hormone in aging male rats: dependence of pharmacological manipulation and endogenous somatostatin release. Neuroendocrinology 47:482-488; 1988
55. Spencer, F M.; Liu, C.C.; Si, C.C.; Howard, G.A. In vivo actions of insulin-like growth factor-I (IGF-I) on bone formation and resorption in rats. Bone 12:21-26; 1991.
56. Takahashi, Y.; Ebihara, S.; Nakamura, Y.; Takahashi, K. A model of human sleep-related growth hormone secretion in dogs: effects of 3, 6 and 12 hours of forced wakefulness on plasma growth hormone, cortisol, and sleep stages. Endocrinology 109:262-272; 1981.
57. Ullman, M.; Oldfors, A Effects of growth hormone on skeletal muscle. I: Studies on normal adult rats. Acta Physiol. Scand. 135:531-536; 1989.
58. Underwood, L.E. Assessment of the risks of treatment with human growth hormone. In: Bercu, B., ed. Basic and clinical aspects of growth hormone. New York: Plenum Press, 1988:357-366.
59. Valcavi, R.; Dieguez, C., Page, M.D.; Zini, M.; Casoli, P.; Portioli, I.; Scanlon, M.F. Alpha-2-adrenergic pathways release growth hormone via a non-GRF-dependent mechanism in normal human subjects. Clin. Endocrinol. 29:309-314; 1988.
60. Veldhuis, J.; Johnson, M.L. Cluster analysis: a simple, versatile and robust algorithm for endocrine pulse detection. Am. J. Physiol. 250:E486-E493; 1986.
61. Vermeulen, A. Nyctohemeral growth hormone profiles in young and aged men: correlation with somatomedin-C levels. J. Clin Endocrinol. Metab. 64:884-888; 1987.
62. Wehrenberg, W B.; Brazeau, P.; Ling, N.; Textor, G.; Guillemin, R Pituitary growth hormone response in rats during a 24-hour infusion of growth hormone-releasing factor. Endocrinology 114:1613-1616; 1984.
63. Williams, E.A.; Kelly, P.J. Age-related bone loss in the dog Trans. Orthopaed Res. Soc. 28:81; 1982
64. Winer, L.M.; Shaw, M.A.; Bauman, G. Basal plasma growth hormone levels in man, new evidence for rhythmicity of growth hormone secretion J. Clin. Endocrinol. Metab 70:1678-1686; 1990.
65. Wüster, C Growth hormone and bone metabolism. Acta Endocrinol. 128:14-18; 1993.