A ferric reductase activity is found in brush border membrane vesicles isolated from Caco-2 cells

Journal of Nutrition

Volumn 126, Issue 9, 1996, Pages 2209-2217

  Ekmekcioglu, Cem ^a   Feyertag, Josef ^a   Marktl, Wolfgang ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

Caco 2 cells; ferric reductase; microvillous membranes; superoxide

Indexed keywords

FERRIC ION; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BRUSH BORDER; ELECTRON TRANSPORT; ENZYME ACTIVITY; HUMAN; HUMAN CELL; INTESTINE CELL; IRON KINETICS; IRON TRANSPORT; MEMBRANE VESICLE; OXIDATION REDUCTION REACTION; SMALL INTESTINE MUCOSA;

EID: 0029831341     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/126.9.2209     Document Type: Article

References (35)

1. Azzi, A., Montecucco C. & Richter, C. (1975) The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes. Biochem. Biophys. Res. Commun. 65: 597-603.
2. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Anal. Biochem. 72: 248-54.
3. Conrad, M. E. &. Umbreit, J. N. (1993) A concise review: iron absorption - the mucin-mobilferrin-integrin pathway. A competitive pathway for metal absorption. Am. J. Hematol. 42: 67-73.
4. Crane, F. L., Sun, I. L., Clark, M. G., Grebing, C. &. Löw, H. (1985) Transplasma-membrane redox systems in growth and development. Biochim. Biophys. Acta 811: 233-264.
5. Dahm, L. J. &. Jones, D. P. (1994) Secretion of cysteine and glutathione from mucosa to lumen in rat small intestine. Am. J. Physiol. 267: G292-G300.
6. Dancis, A., Roman, D. G., Anderson, G. J., Hinnebusch, A. G. & Klausner, R. D. (1992) Ferric reductase of Saccharomyces cerevisae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc. Natl. Acad. Sci. U.S.A. 89:3869-3873.
7. Dixon, M. (1953) The determination of enzyme inhibitor constants. Biochem. J. 55: 170-171.
8. Eadie, G. S. (1942) The inhibition of cholinesterase by physostigmine and prostigmine. J. Biol. Chem. 146: 85-93.
9. Eichholz, A. (1967) Structural and functional organization of the brush border of intestinal epithelial cells. Biochim. Biophys. Acta 135: 475-482.
10. Eide, D., Davis-Kaplan, S., Jordan, I., Sipe, D. & Kaplan, J. (1992) Regulation of iron uptake in Saccharomyces cerevisae. J. Biol. Chem 267: 20774-20781.
11. Fiske, C. H. & Subbarow, Y. (1925) The colorometric determination of phosphorus. J. Biol. Chem. 66: 375-400.
12. Fleet, J. C., Turnbull, A. J., Bourcier, M. & Wood, R. J. (1993) Vitamin D-sensitive and quinacrine-sensitive zinc transport in human intestinal cell line Caco-2. Am. J. Physiol. 264: G1037-G1045.
13. Fleischer, S. & Fleischer, B. (1967) Removal and binding of polar lipids in mitochondria and other membrane systems. In: Methods in Enzymology (Estabrook, R. W. & Pullmann, M. E., eds.) pp. 406-433. Academic Press: New York, NY.
14. Garen, A. & Levinthal, C. (1960) A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. Biochim. Biophys. Acta 38: 470-483.
15. Han, O., Failla, M. L., Hill, A. D., Morris, E. R. & Smith, J. C., Jr. (1995) Reduction of Fe(III) is required for uptake of nonheme iron by Caco-2 cells. J. Nutr. 125: 1291-1299.
16. Hasset, R. & Kosman, D. J. (1995) Evidence for Cu(II) reduction as a component of copper uptake by Saccharomyces cerevisae. J. Biol. Chem. 270: 128-134.
17. m in enzyme reactions. Science (Washington, DC) 116:329-331.
18. 2-generating enzyme activity in rat small intestine. Acta Histochem. Cytochem. 14: 727-733.
19. Kessler, M., Acuto, O., Storelli, C, Murer, H., Muller, M. & Semenza, G. (1978) A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of d-glucose and choline transport systems. Biochim. Biophys. Acta 506: 136-54.
20. Lineweaver, H. & Burk, D. (1934) Determination of enzyme dissociation constants. J. Am. Chem. Soc. 56: 658-666.
21. Mahraoui, L., Rousset, M., Dussaulx, E., Darmoul, D., Zweibaum, A. & Brot-Laroche, E. (1992) Expression and localization of GLUT-5 in Caco-2 cells, human small intestine, and colon. Am. J. Physiol. 263: G312-G318.
22. McCord, J. M. & Fridovich, I. (1969) Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049-6055.
23. Messer, M. &. Dahlquist, A. (1966) A one-step ultramicro method for the assay of intestinal disaccharidases. Anal. Biochem. 14: 376-392.
24. Misra, H. P. (1974) Generation of superoxide free radical during the autooxidation of thiols. J. Biol. Chem. 249: 2151-2155.
25. Morel, F., Dianoux, A. C. & Vignais, P. V. (1988) Superoxide anion measurement by sulfonated phenyl isothiocyanate cytochrome c. Biochem. Biophys. Res. Commun. 156: 1175-1181.
26. 3+ uptake by transformed human intestinal epithelial (Caco-2) cells. Am. J. Physiol. 267: C1582-C1588.
27. Pinto, M., Robine-Leon, S., Appay, M. D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assman, P., Haffen, K., Fogh, J. & Zweibaum, A. (1983) Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 477: 323-333.
28. Pountney, D.J., Simpson, R.J. & Wrigglesworth, J.M. (1994) Duodenal ferric reductase: purification and characterisation. Biochem. Soc. Trans. 22: 284S.
29. Raja, K. B., Simpson, R. J. & Peters, T. J. (1992) Investigation of a role for reduction in ferric iron uptake by mouse duodenum. Biochim. Biophys. Acta 1135: 141-146.
30. Scheiber, B. & Goldenberg, H. (1993) NAD(P)H: ferric iron reductase in endosomal membranes from rat liver. Arch. Biochem. Biophys. 305: 225-230.
31. 3+ transport across isolated intestinal brush border membrane of the mouse. Biochim. Biophys. Acta 772: 220-226.
32. Sottocasa, G. L., Kuylenstierna, B., Ernster, L. & Bergstrand, A. (1967) An electron transport system associated with the outer membrane of liver mitochondria. J. Cell Biol. 32: 415-438.
33. Stookey, L. L. (1970) Ferrozine - a new spectrophotometric reagent for iron. Anal. Chem. 42: 7779-7781.
34. Sun, I. L., Crane, F. L., Grebing, C. & Löw, H. (1985) Transmembrane redox in control of cell growth. Stimulation of HeLa cell growth by ferricyanide and insulin. Exp. Cell. Res. 156: 528-536.
35. Tate, S. S., Grau, E. M. & Meister, A. (1979) Conversion of glutathione to glutathione disulfide by cell membrane-bound oxidase activity. Proc. Natl. Acad. Sci. U.S.A. 76: 2715-2719.