Confocal microscopic detection of potential-sensitive dyes used to reveal loss of voltage control during patch-clamp experiments

Pflugers Archiv European Journal of Physiology

Volumn 433, Issue 1-2, 1996, Pages 194-199

  Hüser, Jörg ^a,b,c   Lipp, Peter ^a   Niggli, Ernst ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

^c RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Cardiac muscle; Confocal microscopy; Di 8 ANEPPS; Voltage clamp; Voltage sensitive fluorescent indicators

Indexed keywords

DYE; SODIUM CHANNEL;

ANIMAL CELL; ARTICLE; CONFOCAL LASER MICROSCOPY; FLUORESCENCE; HEART MUSCLE CELL; INSECT; MEMBRANE CURRENT; MEMBRANE POTENTIAL; NONHUMAN; PRIORITY JOURNAL; VOLTAGE CLAMP;

ANIMALS; CELL LINE; CELL SEPARATION; ELECTROPHYSIOLOGY; FLUORESCENT DYES; GUINEA PIGS; LITHIUM; MICROSCOPY, CONFOCAL; PAPILLARY MUSCLES; PATCH-CLAMP TECHNIQUES; PYRIDINIUM COMPOUNDS; SODIUM; SODIUM CHANNELS; SPODOPTERA;

EID: 0029831276     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050267     Document Type: Article

References (13)

1. Bouchard RA, Clark RB, Giles WR (1993) Role of sodium-calcium exchange in activation of contraction in rat ventricle. J Physiol 472: 391-413
2. Fast VG, Kleber AG (1993) Microscopic conduction in cultured strands of neonatal rat heart cells measured with voltage-sensitive dyes. Circ Res 73: 914-925
3. Gilbert JC, Shirayama T, Pappano, AJ (1991) Inositol trisphosphate promotes Na-Ca exchange current by releasing calcium from sarcoplasmic reticulum in cardiac myocytes. Circ Res 69:1632-1639.
4. Gross E, Bedlack RS, Loew LM (1994) Dual-wavelength ratiometric fluorescence measurement of the membrane dipole potential. Biophys J 67: 208-216
5. 2+-measurements with visible wavelength indicators in isolated cardiac myocytes. Cell Calcium 14: 359-372
6. Lipp P, Niggli E (1994) Sodium current-induced calcium signals in isolated guinea-pig ventricular myocytes. J Physiol 474:439-446
7. Loew LW (1993) Confocal microscopy of potentiometric fluorescent dyes. Methods in Cell Biology 38: 195-209
8. Minta A, Kao JPY, Tsien RY (1989) Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem 264: 8171-8178
9. Niggli E, Piston DW, Kirby MS, Cheng H, Sandison DR, Webb WW, Lederer WJ (1994). A confocal laser scanning microscope designed for indicators with ultraviolet excitation wavelengths. Am J Physiol 266: C303-C310
10. Niggli E, Hüser J, Lipp P (1994) Confocal microscopic detection of membrane potential. Biophys J 66: A94
11. Rohr S, Salzberg BM (1994) Multiple site optical recording of transmembrane voltage (MSORTV) in patterned growth heart cell cultures: assessing electrical behavior, with microsecond resolution, on a cellular and subcellular scale. Biophys J 67: 1301-1315
12. Smith GE, Ju G, Ericson BL, Moschera J, Lahm H, Chizzonite R, Summers MD (1985) Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc Nat Acad Sci USA 82: 8404-8408
13. Stuart GJ, Sakmann B (1994) Active propagation of somatic action potentials into neocortical pyramidal cell dendrites. Nature 367: 69-72