EWS/FLI-1 antagonists induce growth inhibition of Ewing tumor cells in vitro

Cell Growth and Differentiation

Volumn 7, Issue 4, 1996, Pages 429-437

  Kovar, Heinrich ^a   Aryee, Dave N T ^a   Jug, Gunhild ^a   Henöckl, Christian ^a   Schemper, Michael ^c   Delattre, Olivier ^b   Thomas, Gilles ^b   Gadner, Helmut ^a  

^a ST ANNA CHILDREN S HOSPITAL   (Austria)

^b INSTITUT CURIE   (France)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER GROWTH; CONTROLLED STUDY; EWING SARCOMA; GENE ACTIVATION; GENE REARRANGEMENT; GROWTH INHIBITION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTO ONCOGENE;

BETA-GALACTOSIDASE; BLOTTING, NORTHERN; CELL DIVISION; DNA, COMPLEMENTARY; DNA-BINDING PROTEINS; EARLY GROWTH RESPONSE PROTEIN 1; GENE REARRANGEMENT; HUMANS; IMMEDIATE-EARLY PROTEINS; NEUROBLASTOMA; PLASMIDS; PROTO-ONCOGENE PROTEIN C-ETS-2; PROTO-ONCOGENE PROTEIN C-FLI-1; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-ETS; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; RETROVIRIDAE PROTEINS, ONCOGENIC; RNA, ANTISENSE; SARCOMA, EWING'S; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; TRANSFECTION; TUMOR CELLS, CULTURED;

BACTERIA (MICROORGANISMS);

EID: 0029829794     PISSN: 10449523     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (45)

1. Boulukos, K E, Pognonec, P., Rabault, B., Begue, A., Ghysdael, J. Definition of an Ets1 protein domain required for nuclear localization in cells and DNA-binding activity in vitro. Mol Cell. Biol., 9: 5718-5721, 1989.
2. Wasylyk, B., Hahn, S. L., and Giovane, A. The Ets family of transcription factors. Eur J. Biochem., 277: 7-18, 1993.
3. Moreau-Gachelin, F., Tavitian, A., and Tambourin, P. Spi-1 is a putative oncogens in virally induced murine erythroleukaemias. Nature (Lond.), 331: 277-280, 1988.
4. Moreau-Gachelin, F., Ray, D., Mattei, M G., Tambourin, P., and Tavitian, A. The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias. Oncogene, 4: 1449-1456, 1989.
5. Ben-David, Y., Giddens, E. B., Letwin, K., and Bernstein, A. Erythroleukemia induction by Friend murine leukemia virus: insertional activation of a new member of the ets gene family, Fli-1, closely linked to c-ets. Genes & Dev., 5: 908-918, 1991
6. Howard, J C, Yousefi, S., Cheong, G., Bernstein, A., and Ben-David, Y. Temporal order and functional analysis of mutations within the Fli-1 and p53 genes during the erythroleukemias induced by F-MuLV. Oncogene, 8: 2721-2729, 1993.
7. Delattre, O., Zucman, J., Plougastel, B., Desmaze, C , Melot, T., Peter, M., Kovar, H., Joubert, I., de Jong, P , Rouleau, G., Aurias, A., and Thomas, G. Gene fusion with an ETS DNA binding domain caused by chromosome translocation in human tumors. Nature (Lond.), 359: 162-165, 1992.
8. Zucman, J., Melot, T., Desmaze, C , Ghysdael, J., Plougastel, B., Peter, M., Zucker, J. M., Triche, T. J., Sheer, D., Turc-Carel, C., Ambras, P., Combaret, V , Lenoir, G., Aurias, A., Thomas, G., and Delattre, O. Combinatorial generation of variable fusion proteins in the Ewing family of tumors. EMBO J., 12: 4481-4487, 1993.
9. Triche, T. J. Pathology of pediatric malignancies. In: P. A. Pizzo and D. G. Poplack, (eds.), Principles and Practice of Pediatric Oncology, pp. 115-152. Philadelphia: J. B. Lippincott, 1993.
10. Cavazzana, A. O., Miser, J. S , Jefferson, J., and Triche, T. J. Experimental evidence for a neural origin of Ewing's sarcoma of bone. Am. J. Pathol., 127: 507-518, 1987.
11. Dehner, L., P. Primitive neuroectodermal tumor and Ewing's sarcoma. Am. J. Surg. Pathol., 17; 1-13, 1993.
12. Dellagi, K., Lipinski, M., Paulin, D., Portier, M. M., Lenoir, G. M., and Brouet, J. C. Characterization of intermediate filaments expressed by Ewing tumor cell lines. Cancer Res., 47: 1170-1173, 1987
13. Dickman, P. S., Liotta, L. A., and Triche, T. J. Ewing's sarcoma: characterization in established cultures and evidence of its histogenesis. Lab. Invest., 47: 375-382, 1982.
14. Miettinen, M., Lehto, V. P., and Virtanen, I Histogenesis of Ewing's sarcoma: an evaluation of intermediate filaments and endothelial markers. Virchows Arch., 41: 277-284, 1982.
15. Kovar, H., Dworzak, M., Strehl, S., Schnell, E., Ambras, I. M., Ambros, P. F., and Gadner, H. Overexpression of the pseudoautosomal gene MIC2 in Ewing's sarcoma and peripheral primitive neuroectodermal tumor. Oncogene, 5: 1067-1070, 1990.
16. Dworzak, M. N., Fritsch, G., Buchinger, P., Fleischer, C , Printz, D., Zellner, A., Schollhammer, A., Steiner, G., Ambros, P. F., and Gadner, H. Flow cytometric assessment of human MIC2 expression in bone marrow, thymus and peripheral blood. Blood, 83: 415-425, 1994.
17. Turc-Carel, C., Aurias, A., Mugneret, F., Lizard, S., Sidaner, I., Volk, C., Thiery, J. P., Olschwang, S., Philip, I., Berger, M. P., Philip, T., Lenoir, G. M., and Mazabraud, A. Chromosomes in Ewing's sarcoma. I. An evaluation of 85 cases and remarkable consistency of t(11;22)(q24;q12). Cancer Genet. Cytogenet., 32: 229-238, 1988.
18. Delattre, O., Zucman, J., Melot, T , Sastre, X., Zucker, J. M., Lenoir, G., Ambros, P , Sheer, D., Turc-Carel, C., Triche, T. J., Aurias, A., and Thomas, G. The Ewing family of tumors: a subgroup of small round cell tumors defined by specific chimeric transcripts. N Engl. J. Med., 331: 294-299, 1994.
19. Zoubek, A., Pfleiderer, C., Salzer-Kuntschik, M., Amann, G., Windhager, R., Fink, F. M., Koscelniak, E., Delattre, O., Strehl, S., Ambros, P. F., Gadner, H., and Kovar, H. Variability of EWS-chimaeric transcripts in Ewing tumors: a comparison of clinical and molecular data. Br. J. Cancer, 70: 908-913, 1994.
20. May, W. A., Gishizky, M. L., Lessnick, S. L., Lunsford, L. B., Lewis, B. C., Delattre, O., Zucman, J., Thomas, G., and Denny, C. T. Ewing's sarcoma 11,22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation. Proc. Natl. Acad. Sci. USA, 90: 5752-5756, 1993.
21. May, W. A., Lessnick, S. L., Braun, B. S., Klemsz, M., Lewis, B. C., Lunsford, L. B., Hromas, R., and Denny, C. T The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1. Mol. Cell. Biol , 13: 7393-7398, 1993.
22. Ohno, T , Rao, V. N., and Reddy, E. S. EWS/Fli-1 chimeric protein is a transcriptional activator. Cancer Res., 53: 5859-5863, 1993.
23. Bailly, R-A., Bosselut, R., Zucman, J., Cormier, F., Delattre, O., Roussel, M , Thomas, G., and Ghysdael, J. DNA-binding and transcriptional activation properties of the EWS-Fli-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma. Mol. Cell. Biol., 14: 3230-3241, 1994.
24. Watson. D. K., McWilliams, M. J., Lapis, P., Lautenberger, J. A, Schweinfest, C. W , and Papas, T. S. Mammalian ets-1 and ets-2 genes encode highly conserved proteins. Proc. Natl. Acad. Sci. USA, 85: 7862-7866, 1988.
25. Watanabe, H., Sawada, J., Yano, K., Yamaguchi, K., Goto, M., and Handa, H. cDNA cloning of transcription factor E4TF1 subunits with ets and notch motifs. Mol. Cell. Biol , 13: 1385-1391, 1993.
26. Rao, V. N., Huebner, K., Isobe, M., Ar-Rushdi, A., Croce, C. M., and Reddy, E. S. P. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints. Science (Washington DC), 244: 66-70, 1989.
27. Brown, T. A., and McKnight, S. L. Specificities of protein-protein and protein-DNA interactions of GABPα and two newly defined ets-related proteins. Genes & Dev , 6: 2502-2512, 1992.
28. Thompson, C. B., Wang, C-Y., Ho, I. C., Bohjanen, P. R., Petryniak, B., June, C. H., Miesfeldt, S., Zhang, L., Nabel, G. J., Karpinski, B., and Leiden, J. M. cis-acting sequences required for inducible interleukin-2 enhancer function bind a novel ets-related protein, elf-1. Mol. Cell. Biol., 12: 1043-1053, 1992.
29. Kalderon, D., Roberts, B. L., Richardson, W. D., and Smith, A. E. A short amino acid sequence able to specify nuclear location. Cell, 39: 499-509, 1984.
30. Rao, V. N., Ohno, T., Prasad, D. D., Bhattacharya, G., and Reddy, E. S. Analysis of the DNA-binding and transcriptional activation functions of human Fli-1 protein. Oncogene, 8: 2167-2173, 1993.
31. Siddique, H. R., Rao, V. N., Lee, L., and Reddy, E. S Characterization of the DNA binding and transcriptional activation domains of the erg protein. Oncogene, 8: 1751-1755, 1993.
32. Reddy, E. S., and Rao, V. N. erg, an ets-related gene, codes for sequence specific transcriptional activators. Oncogene, 6: 2285-2289, 1991.
33. Zhang, L., Lemarchandel, V , Romeo, P. H., Ben David, Y., Greer, P., and Bernstein, A. The Fli-1 proto-oncogene, involved in erythroleukemia and Ewing's sarcoma, encodes a transcriptional activator with DNA-binding specificities distinct from other Ets family members. Oncogene, 8: 1621-1630, 1993.
34. Neckers, L., Whitesell, L., Rosolen, A., and Geselowitz, D A. Antisense inhibition of oncogene expression. Crit. Rev. Oncog., 3: 175-231, 1992.
35. Brewer, G., and Ros, J. Poly(A) shortening and degradation of 3′ A+U-rich sequences of human c-myc mRNA in a cell-free system. Mol. Cell. Biol., 8: 1697-1708, 1988.
36. Mao, X., Miesfeldt, S., Yang, H., Leiden, J. L., and Thompson, C. B. The FLI-1 and EWS-FLI-1 oncoproteins display similar DNA binding specificities. J. Biol. Chem., 269: 18216-18222, 1994.
37. Kovar, H., Auinger, A., Jug, G., Aryee, D., Zoubek, A., Salzer-Kuntschik, M., and Gadner, H. Narrow spectrum of infrequent p53 mutations and absence of MDM2 amplification in Ewing tumors. Oncogene, 8: 2683-2690, 1993.
38. Baker, S. J., Markowitz, S., Fearon, E. R., Willson, J. K., and Vogelstein, B. Suppression of human colorectal carcinoma cell growth by wild-type p53. Science (Washington DC), 249: 912-915, 1990.
39. Ruther, U., and Muller-Hill, B. Easy identification of cDNA clones. EMBO J., 2: 1791-1794, 1983.
40. MacGregor, G. R., and Caskey, C. T. Construction of plasmids that express E. coli β-galactosidase in mammalian cells. Nucleic Acids Res., 17: 2365, 1989.
41. Felgner, P. L., Gadek, T. R., Holm, M , Roman, R., Chan, H. W., Wenz, M., Northrop, J. P , Ringold, G. M., and Danielsen, M. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci USA, 84: 7413-7417, 1987.
42. Cotten, M., Langle-Rouault, F., Kirlappos, H., Wagner, E., Mechtler, K., Zenke, M., Beug, H , and Bimstiel, M. L. Transferrin-polycation-mediated introduction of DNA into human leukemia cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels. Proc. Natl. Acad. Sci. USA, 87: 4033-4037, 1990.
43. Lim, K , and Chae, C. B. A simple assay for DNA transfection by incubation of the cells in culture dishes with substrates for β-galactosidase. BioTechniques, 7: 576-579, 1989.
44. Kirk, R. E. Experimental Design, Ed. 2. Belmont, CA: Wadsworth, 1982.
45. SAS, SAS/STAT User's Guide, Version 6, SAS Institute, Cary, NC, 1991.