Staining paraffin sections without prior removal of the wax

Biotechnic and Histochemistry

Volumn 71, Issue 6, 1996, Pages 304-310

  Kiernan, J A ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

methods paraffin wax; sections; staining

Indexed keywords

PARAFFIN; WAX;

ANIMAL TISSUE; ARTICLE; HISTOLOGY; NONHUMAN; STAINING; TISSUE PREPARATION; TISSUE SECTION;

ANIMALIA;

EID: 0029829517     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299609117180     Document Type: Article

References (26)

1. Allison, R. and Tanswell, S. 1993. Unexpected results of trichrome staining of quenched epithelial tissue following delayed fixation. J. Histotechnol. 16: 343-348.
2. Baker, J. R. 1958. Principles of Biological Microtechnique (Reprinted 1970, with corrections). Methuen, London. pp. 303-304.
3. Baker, J. R. 1962. Experiments on the action of mordants. 2. Aluminium-haematein. Q. J. Microsc. Sci. 103: 493-517.
4. Bronner, R. 1975. Simultaneous demonstration of lipids and starch in plant tissue. Stain Technol. 50: 1-4.
5. Clark, G. 1981. Staining Procedures Used by the Biological Stain Commission, 4th ed. Williams & Wilkins. Baltimore. p. 48.
6. Culling, C. F. A. 1974. Handbook of Histopathological and Histochemical Techniques, 3rd ed. Butterworths, London, p. 141.
7. Gabe, M. 1976. Histological Techniques. Blackith. E. and Kavoor, A., Eds., Masson, Paris, pp. 217-219.
8. Gill, G. W., Frost, J. K. and Miller. K. A. 1974. A new formula for a half-oxidized hematoxylin solution that neither overstains nor requires differentiation. Acta Cytol. 18: 300-311.
9. Goetsch. J. B., Reynolds, P. M. and Bunting, H. 1952. Modification of Gomori method for alkaline and acid phosphatase avoiding artefact staining of nucleus. Proc. Soc. Exp. Biol. Med. 80: 71-75.
10. Goldstein, D. J. 1963. An approach to the thermodynamics of histological dyeing, illustrated by experiments with azure A. Q. J. Microsc. Sci. 104: 413-439.
11. Graham, E. T. and Joshi, P. A. 1995. Novel fixation of plant tissue, staining through paraffin with alcian blue and hematoxylin, and improved slide preparation. Biotechnic & Histochemistry 70: 263-266.
12. Horobin, R. W. 1982. Histochemistry: an Explanatory Outline of Histochemistry and Biophysical Staining. Gustav Fischer, Stuttgart.
13. Horobin, R. W. 1988. Understanding Histochemistry: Selection, Evaluation and Design of Biological Stains. Ellis Horwood, Chichester.
14. Horobin, R. W. and Flemming, L. 1988. One-bath trichrome staining: investigation of a general mechanism based on a structure-staining correlation analysis. Histochem. J. 20: 23-34.
15. Horobin, R. W. and Tomlinson, A. 1976. The influence of the embedding medium when staining sections for electron microscopy: the penetration of stains into plastic sections. J. Microsc. 108: 69-78.
16. Humason, G. L. 1979. Animal Tissue Techniques, 4th ed. Freeman, San Francisco, p. 61.
17. Kashiwa. H. K. and House, C. M. 1964. The glyoxal bis(2-hydroxyanil) method modified for localizing insoluble calcium salts. Stain Technol. 39: 359-367.
18. Kiernan, J. A. 1990. Histological and Histochemical Methods: Theory and Practice. 2nd ed. Pergamon Press, Oxford, pp. 25, 35.
19. Mallory, F. B. 1938. Pathological Technique. Hafner, New York.
20. McFarland, F. M. 1922. Some simplifications of microscopical technique. Science 56: 43-44.
21. Reid, P. E., Iagallo, M., Nehr, S., Jankunis, M., Morrow, L. and Trueman, L. 1993. Mechanism of connective tissue techniques. 1. The effect of dye concentration and staining time on anionic dye procedures. Histochem. J. 25: 821-829.
22. Ruyter, J. H. C. and Neumann, H. 1949. A critical examination of the histochemical demonstration of the alkaline phosphomonoesterase. Biochim. Biophys. Acta 3: 125-134.
23. Sakai, W. S. 1973. Simple method for differential staining of paraffin embedded plant material using toluidine blue O. Stain Technol. 48: 247-249.
24. Williams, M. G. and Adrian, E. K. 1977. The use of elemental iodine to enhance staining of thin sections to be viewed in the electron microscope. Stain Technol. 52: 269-272.
25. Wolters, G. H. J., Pasma, A., Konijnendijk, W. and Bouman, P. R. 1979. Evaluation of the glyoxalbis-(2-hydroxyanil)-method for staining of calcium in model gelatin films and pancreatic islets. Histochemistry 62: 137-151.
26. Yensen, J. 1968. Removal of epoxy resin from histological sections following halogenation. Stain Technol. 43: 344-346.