Self-sterility in Arabidopsis due to defective pollen tube guidance

Science

Volumn 274, Issue 5292, 1996, Pages 1535-1537

  Wilhelmi, Laura K ^a   Preuss, Daphne ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARABIDOPSIS; ARTICLE; CELL INTERACTION; DIPLOIDY; GENE MAPPING; GENE MUTATION; GENOTYPE; GERMINATION; INHERITANCE; NONHUMAN; PHENOTYPE; PLANT GROWTH; POLLEN; PRIORITY JOURNAL; REPRODUCTION;

ARABIDOPSIS; CROSSES, GENETIC; GENES, PLANT; GENETIC MARKERS; GENOTYPE; MICROSCOPY, ELECTRON, SCANNING; MUTATION; PHENOTYPE; POLLEN; REPRODUCTION;

ARABIDOPSIS; MAGNOLIOPHYTA;

EID: 0029828840     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5292.1535     Document Type: Article

References (25)

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15. Of crosses between emasculated mutant pistils and wild-type pollen, 116/133 were successful, and of crosses between emasculated wild-type pistils and mutant pollen, 76/91 were successful, with average seed sets of 41 ± 8 (n = 11) and 31 ± 15 (n = 37), respectively; wild-type Arabidopsis pistils contain about 50 ovules. Similar pollinations with mutant pollen and mutant pistils yielded no seed (n = 52).
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19. 2 plants were scored. Recombination break-point analysis was used to map POP2 to chromosome 3 at 37.8 ± 1.3 cM (within a 4.8-cM interval between m560 and DMC1) and POP3 to a 6-cM interval between nga106 and Pl (genetic distances correspond to those reported in September 1996 by C. Lister and C. Dean, available at http://genome-www.stanford. edu/Arabidopsis/ww/Vol3ii/home.html). No loci, other than those indicated in Fig. 2, showed significant linkage to the sterile phenotype.
20. 2 plants were scored. Recombination break-point analysis was used to map POP2 to chromosome 3 at 37.8 ± 1.3 cM (within a 4.8-cM interval between m560 and DMC1) and POP3 to a 6-cM interval between nga106 and Pl (genetic distances correspond to those reported in September 1996 by C. Lister and C. Dean, available at http://genome-www.stanford. edu/Arabidopsis/ww/Vol3ii/home.html). No loci, other than those indicated in Fig. 2, showed significant linkage to the sterile phenotype.
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22. 2 or ∼9%.
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25. We thank W. E. Browne, D. Charlesworth, R. W. Davis, C. Gasser, B. Hauser, A. Shapiro, V. Walbot, and O. Rossanese for helpful discussions; G. Copenhaver, B. Keith, S. Kron, J. Mayfield, and L. Mets for critical reading of the manuscript; and L. DuPree and R. Choi for technical assistance. The Arabidopsis Biological Resource Center provided ms1 seeds. Supported by an NIH Genetics and Regulation Training Grant (L.K.W.) and by grants from the American Cancer Society, U.S. Department of Energy. and NSF (D.P.). This project was initially supported by R. W. Davis (NSF grant DMB-9106011).