The measurement of pyridinium crosslinks: A methodological overview

Annals of Clinical Biochemistry

Volumn 33, Issue 5, 1996, Pages 397-420

  James, I T ^a   Walne, A J ^a   Perrett, D ^a  

^a ST BARTHOLOMEW S HOSPITAL   (United Kingdom)

Author keywords

Collagen crosslinks; Deoxypyridinoline; HPLC; Pyridinoline

Indexed keywords

BIOLOGICAL MARKER; PYRIDINIUM DERIVATIVE;

CHROMATOGRAPHY; COLLAGEN METABOLISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOASSAY; PRIORITY JOURNAL; PROTEIN CROSS LINKING; REVIEW; TISSUE DISTRIBUTION;

EID: 0029828170     PISSN: 00045632     EISSN: None     Source Type: Journal    
DOI: 10.1177/000456329603300503     Document Type: Review

References (146)

1. Harkness MCR, Harkness RD, James DW. The effect of a protein diet on the collagen content of mice. J Physiol 1958; 144: 307-13
2. Rehn M, Pihlajaniemi T. Identification of three n-terminal ends of type XVIII collagen chains and tissue-specific differences in the expression of the corresponding transcripts-the longest form contains a novel motif homologous to rat and drosophila frizzled proteins. J Biol Chem 1995; 270: 4705-11
3. Prockop DJ. Mutations in collagen genes as a cause of connective tissue disease. N Engl J Med 1992; 326: 540-6
4. Byers PH. Disorders of collagen biosynthesis and structure. In: Scriver CR, Beaudet AL, Sly WS, Valle D, eds. The Metabolic Basis Of Inherited Disease, 6th edn, Vol 2, New York: McGraw-Hill, 1989; 2805-42
5. Kagan HM, Sullivan KA. Lysyl oxidase: preparation and role in elastin biosynthesis. Methods Enzymol 1982; 82(A): 637-50
6. Eyre DR, Paz MA, Gallop PM. Crosslinking in collagen and elastin. Annu Rev Biochem 1984; 53: 717-48
7. Otsubo K, Katz EP, Mechanic GL, Yamauchi M. Cross-linking connectivity in bone collagen fibrils: the COOH-terminal locus of free aldehyde. Biochemistry 1992; 31: 396-402
8. Fujimoto D, Moriguchi T, Ishida T, Hayashi H. The structure of pyridinoline, a collagen crosslink. Biochem Biophys Res Commun 1978; 84: 52-7
9. Eyre DR, Oguchi H. The hydroxypyridinium crosslinks of skeletal collagens: Their measurement, properties and a proposed pathway of formation. Biochem Biophvs Res Commun 1980; 92: 403-10
10. Robins SP, Duncan A. Cross-linking of collagen. Location of pyridinoline in bovine articular cartilage at two sites of the molecule. Biochem J 1983; 215: 175-82
11. Robins SP. Functional properties of collagen and elastin. In: Dixon J, Bird H, eds. Bailliere's Clinical Rheumatology: Biochemical Aspects Of Rheumatic Diseases. 2nd edn. London: Bailliere Tindall, 1988: 1-36
12. Robins SP. Analysis of the crosslinking components in collagen and elastin. Methods Biochem Anal 1982; 28: 329-79
13. Eyre DR. Collagen crosslinking amino acids. Methods Enzymol 1987; 144: 115-39
14. Ricard-Blum S, Ville G. Collagen crosslinking. Cell Mol Biol 1988; 34: 581-90
15. Beardsworth LJ, Eyre DR, Dickson IR. Changes with age in the urinary excretion of lysyl- and hydroxylysylpyridinoline, two new markers of bone collagen turnover. J Bone Miner Res 1990; 5: 671-6
16. 1(I) chain of dermosparatic calf skin procollagen. FEBS Lett 1978; 89: 279-82
17. Porter RR, Reid KBM. The biochemistry of complement. Nature 1978; 275: 699-704
18. Bienkowski RS, Cowan MJ, McDonald JA, Crystal RG. Degradation of newly synthesised collagen. J Biol Chem 1978; 253: 4356-63
19. Gasser A, Celada A, Courvoisier B, Depierre D, Hulme PM, Rinsler M, et al. The clinical measurement of urinary total hydroxyproline excretion. Clin Chim Acta 1979; 95: 487-91
20. Teerlink T, Tavenier P, Netelenbos JC. Selective determination of hydroxyproline in urine by high performance liquid chromatography using pre-column derivatisation. Clin Chim Acta 1989; 183: 309-16
21. Delmas PD. Clinical use of biochemical markers of bone remodelling in osteoporosis. In: Christiansen C, Overgaard, K, eds. Osteoporosis 1990. Copenhagen: Osteopress ApS, 1990: 450-8
22. Pinnell SR, Fox R, Krane SM. Human collagens: differences in glycosylated hydroxylysines in skin and bone. Biochim Biophys Acta 1971; 229: 119-22
23. Krane SM, Kantrowitz FG, Byrne M, Pinnell SR, Singer FR. Urinary excretion of hydroxylysine and its glycosides as an index of collagen degradation. J Clin Invest 1977; 59: 819-27
24. Segrest JP, Cunningham IW. Variations in human urinary O-hydroxylysyl glycoside levels and their relationship to collagen metabolism. J Clin Invest 1979; 49: 1497-509
25. Moro L, Modricky C, Stagni N, Vittur F, de Bernard B. High performance liquid chromatography analysis of urinary hydroxylysline glycosides as indicators of collagen turnover. Analyst 1984; 109: 1621-8
26. Demers LM. New biochemical marker for bone disease: Is it a breakthrough? Clin Chem 1992; 38: 2169-70
27. Eyre DR. Editorial: New biomarkers of bone resorption. J Clin Endocrinol Metab 1992; 74: 470AC
28. Editorial. Pyridinium crosslinks as markers of bone resorption. Lancet 1992; 340: 278-9
29. Colwell A, Eastell R, Assiri AMA, Russell RGG. Effect of diet on deoxypyridinoline excretion. In: Christiansen C, Overgaard K, eds. Osteoporosis 1990. Copenhagen: Osteopress ApS, 1990: 590-1
30. Stone PJ, Lucey EC, Snider GL, Franzblau C. Effect of diet on urinary excretion of desmosine and hydroxylysyl pyridinoline. Am J Respir Crit Care Med 1994; 149: 174-7
31. Eastell R, Hampton L, Colwell A, Green JR, Assiri AMA, Hesp R, et al. Urinary collagen crosslinks are highly correlated with radioisotopic measurements of bone resorption. In: Christiansen C, Overgaard K, eds. Osteoporosis 1990. Copenhagen: Osteopress ApS, 1990: 469-70
32. Delmas PD, Schlemmer A, Gineyts E, Riis B, Christiansen C. Urinary excretion of pyridinoline crosslinks correlates with bone turnover measured on iliac crest biopsy in patients with vertebral osteoporosis. J Bone Miner Res 1991; 6: 639-44
33. Fujimoto D. Isolation and characterisation of a fluorescent material in bovine achilles tendon collagen. Biochem Biophys Res Commun 1977; 76: 1124-9
34. Barber M, Bordoli RS, Elliott GJ, Fujimoto D, Scott JE. The structure(s) of pyridinoline(s). Biochem Biophys Res Commun 1982; 109: 1041-6
35. Ogawa T, Ono T, Tsuda M, Kawanishi Y. A novel fluor in insoluble collagen: A crosslinking moiety in collagen molecules. Biochem Biophys Res Commun 1982; 107: 1252-7
36. Gunja-Smith Z, Boucek RJ. Collagen crosslinking compounds in human urine. Biochem J 1981; 197: 759-62
37. Robins SP. Cross-linking of collagen. Isolation, structural characterization and glycosylation of pyridinoline. Biochem J 1983; 215: 167-73
38. Fujimoto D. Evidence for natural existence of pyridinoline crosslink in collagen. Biochem Biophys Res Commun 1980; 93: 948-53
39. Sakura S, Fujimoto D, Sakamoto K, Mizuno A, Motegi K. Photolysis of pyridinoline, a cross-linking amino acid of collagen, by ultraviolet light. Can J Biochem 1982; 60: 525-9
40. Robins SP, Duncan A, Riggs BL. Direct measurement of free hydroxypyridinium crosslinks of collagen in urine as new markers of bone resorption in osteoporosis. In: Christiansen C, Overgaard K, eds. Osteoporosis 1990. Copenhagen: Osteopress. ApS, 1990: 465-8
41. Eyre DR, Koob TJ, Van Ness KP. Quantitation of hydroxypyridinium crosslinks in collagen by high-performance liquid chromatography. Anal Biochem 1984; 137: 380-8
42. Walters C, Eyre DR. Collagen crosslinks in human dentin: increasing content of hydroxypyridinium residues with age. Calcif Tissue Int 1983; 35: 401-5
43. Gosselin LE, Martinez DA, Vailas AC, Sieck GC. Passive length-force properties of senescent diaphragm: relationship with collagen characteristics. J Appl Physiol 1994; 76: 2680-5
44. Palokangas H, Kovanen V, Duncan A, Robins SP Age-related changes in the concentration of hydroxypyridinium crosslinks in functionally different skeletal muscles. Matrix 1992; 12: 291-6
45. Zimmerman SD, McCormick RJ, Vadlamudi RK, Thomas DP. Age and training alter collagen characteristics in fast and slow twitch rat limb muscle. J Appl Physiol 1993; 75: 1670-4
46. Fujimoto D, Suzuki M, Uchiyama A, Miyamoto S, Inoue T. Analysis of pyridinoline. a cross-linking compound of collagen fibers, in human urine. J Biochem 1983; 94: 1133-6
47. Black D, Duncan A, Robins SP. Quantitative analysis of the pyridinium crosslinks of collagen in urine using ion-paired reversed-phase high-performance liquid chromatography. Anal Biochem 1998; 169: 197-203
48. Stone PJ, Bryan-Rhadfi J, Shaw HA, Franzblau C. Isolation of hydroxylysyl pyridinoline. a mature collagen crosslink from neonatal rat aorta smooth muscle cell cultures. Matrix 1992; 12: 381-7
49. James IT, Perrett D, Thompson PW. Rapid assay for hard tissue collagen cross-links using isocratic ion-pair reversed-phase liquid Chromatography. J Chromatogr 1990; 525: 43-57
50. Colwell A, Russell RG, Eastell R. Factors affecting the assay of urinary 3-hydroxy pyridinium cross-links of collagen as markers of bone resorption. Eur J Clin Invest 1993; 23: 341-9
51. Kollerup G, Thamsborg G, Bhatia H, Sorensen OH. Quantitation of urinary hydroxypyridinium cross-links from collagen by high-performance liquid chromatography. Scand J Clin Lab Invest 1992; 52: 657-62
52. Kamel S, Brazier M, Desmet G, Picard C, Mennecier I, Sebert J. High-performance liquid Chromatographic determination of 3-hydroxypyridinium derivatives as new markers of bone resorption. J Chromatogr 1992; 574: 255-60
53. Pratt DA, Daniloff Y, Duncan A, Robins SP. Automated analysis of the pyridinium crosslinks of collagen in tissue and urine using solid-phase extraction and reversed-phase high-performance liquid chromatography. Anal Biochem 1992; 207: 168-75
54. Yoshimura Y, Ohnishi K, Hamamura M, Oda T, Sohda T. Automated high-performance liquid Chromatographic determination of hydroxylysylpyridinoline and lysylpyridinoline in urine using a column-switching method. J Chromatogr 1993; 613: 43-9
55. James IT, Walne AJ, Perrett D. Automated solid phase extraction with HPLC analysis of pyridinium crosslinks. Anal Biochem 1996 (in press)
56. Lichy A, Macek J, Adam M Determination of hydroxylysylpyridinoline in tissues and urine by high-performance liquid chromatography. J Chromatogr 1991; 563: 153-7
57. James IT, Perrett D. The use of capillary zone electrophoresis for the measurement of urinary pyridinoline [Abstract]. Clin Rheumatol 1994; 10: 457
58. Colwell A, Eastell R, Al-Dehaimi A, Biggins C, Russell RGG. The measurement of 3-OH pyridinium compounds using high performance capillary electrophoresis. In: Ring EFJ, ed. Current Research in Osteoporosis and Bone Mineral Measurement II: 1992, 2nd edn. London: British Institute of Radiology, 1992: 5-6
59. Eyre DR. Crosslink maturation in bone collagen. In: Veis A, ed. The Chemistry and Biology of Mineralised Connective Tissues. Amsterdam: Elsevier, 1981: 51-5
60. Koob TJ, Eyre DR, Van Ness KP. Detection and photolysis of hydroxypyridinium crosslinks in cartilage collagen in situ. Trans Ann Meet Orthopaed Res, USA 1983; 28
61. Abbiati G, Rigoldi M, Mussini E, Castiglioni C. An HPLC isocratic method for pyridinoline (HP) and deoxypyridinoline (LP) analysis: A new approach to their standard quantification. In: Christiansen C, Overgaard K, eds. Osteoporosis 1990. Copenhagen: Osteopress ApS, 1990: 616-8
62. James I, Crowley C, Perrett D. Assay of pyridinium crosslinks in serum using narrow-bore ion-paired reversed-phase high-performance liquid chromatography. J Chromatogr 1993; 612: 41-8
63. James IT, Perrett D, Skingle L, Grootveld M, Thompson PW. A novel pyridinoline like crosslink in human bone and urine hydrolysates. In: Christiansen C, Overgaard K, eds. Osteoporosis 1990. Copenhagen: Osteopress ApS, 1990: 517-20
64. Skinner SJM. Rapid method for the purification of the elastin crosslinks, desmosine and isodesmosine. J Chromatogr 1982; 229: 200-4
65. Robins SP, McLaren AM, Nicol P, Seibel MJ. Pyridinium crosslink measurements in serum and synovial fluid of patients with osteoarthritis. In: Kuettner KE, Schleyerbach R, Peyron JG, Hascall VC, eds. Articular Cartilage and Osteoarthritis. New York: Raven Press, 1992: 738-9
66. Abbiati G, Rigoldi M, Frignani S, Colombo L, Mussini E. Determination of pyridinium crosslinks in plasma and serum by high-performance liquid chromatography. J Chromatogr 1994; 656: 303-10
67. Walne AJ, James IT, Perrett D. Baseline data for serum pyridinium crosslinks in a normal population [Abstract]. Arthritis Rheum 1995; 38: S356
68. Robins SP. An enzyme-linked immunoassay for the collagen cross-link pyridinoline. Biochem J 1982; 207: 617-20
69. Robins SP, Stewart P, Astbury C, Bird HA. Measurement of the cross linking compound, pyridinoline, in urine as an index of collagen degradation in joint disease. Ann Rheum Dis 1986; 45: 969-73
70. Ohishi T, Takahashi M, Kushida K, Horiuchi K, Ishigaki S, Inoue T. Quantitative analyses of urinary pyridinoline and deoxypyridinoline excretion in patients with hyperthyroidism. Endocr Res 1992; 18: 281-90
71. Gunja-Smith Z. An enzyme-linked immunosorbent assay to quantitate the elastin crosslink desmosine in tissue and urine samples. Anal Biochem 1985; 147: 258-64
72. Calabresi E, Lasagni L, Franceschelli F, Bartolini L, Serio M, Brandi ML. Use of an internal standard to measure pyridinoline and deoxypyridinoline in urine [Letter]. Clin Chem 1994; 40: 336-7
73. Robins SP, Stead DA, Duncan A. Precautions in using an internal standard to measure pyridinoline and deoxypyridinoline in urine. Clin Chem 1994; 40: 2322-3
74. Calabresi E, Lasagni L, Franceschelli F, Bartolini L, Serio M, Brandi ML. Precautions in using an internal standard to measure pyridinoline and deoxypyridinoline in urine. Clin Chem 1994; 40: 2323
75. Risteli J, Elomaa I, Niemi S, Novamo A, Risteli L. Radioimmunoassay for the pyridinoline cross-linked carboxy terminal telopeptide of type I collagen: A new serum marker of bone collagen degradation. Clin Chem 1993; 39: 635-40
76. Bonde M, Qvist P, Fledelius C, Riis BJ, Christiansen C. Immunoassay for quantifying type I collagen degradation products in urine evaluated. Clin Chem 1994; 40: 2022-3
77. Garnero P, Gineyts E, Riou JP, Delmas PD. Assessment of bone resorption with a new marker of collagen degradation in patients with metabolic bone disease. J Clin Endocrinol Metab 1994; 79: 780-5
78. Hanson DA, Weis MA, Bollen AM, Maslan SL, Singer FR, Eyre DR. A specific immunoassay for monitoring human bone resorption: quantitation of type I collagen cross-linked N-telopeptides in urine. J Bone Miner Res 1992; 7: 1251-8
79. Fox P, Stamp TCB. Immunoassay of collagen crosslinks in acidified urine. Ann Clin Biochem 1995; 32: 326-8
80. Robins SP, Woitge H, Hesley R, Ju J, Seyedin S, Seibel MJ. Direct, enzyme-linked immunoassay for urinary deoxypyridinoline as a specific marker for measuring bone resorption. J Bone Miner Res 1994; 9: 1643-9
81. Chen SY, Kline S, Lindsey K, Seyedin S, Hesley R. An automated chemiluminescent assay for deoxypyridinoline: A urinary marker for bone resorption [Abstract]. J Bone Miner Res 1994; 9: A376
82. van Hoek MJC, Martens MFWC, Walchli R, Gamse R, Rosmalen FMA. A novel polyclonal antibody against deoxypyridinoline to be used in a radioimmunoassay [Abstract]. Second Workshop on Bisphosphonates: From the Laboratory to the Patient, Davos, April 14-16 1994. Geneva: Nichols Institute Diagnostics, 1994
83. Urena P, Ferreira A, Kung VT, Morieux C, Simon P, Ang KS, et al. Serum pyridinoline as a specific marker of collagen breakdown and bone metabolism in haemodialysis patients. J Bone Miner Res 1995; 10: 932-9
84. Kung VT, Gomez B, Lungard R, Seyedin S, Clark B, Riggs L. An immunoassay for the measurement of free pyridinoline in serum: a marker of bone resorption [Abstract]. J Bone Miner Res 1993; 8: S151
85. Elomaa I, Virkkunen P, Risteli L, Risteli J. Serum concentration of the crosslinked carboxyterminal telopeptide of type I collagen (ICTP) is a useful prognostic indicator in multiple myeloma. Br J Cancer 1992; 66: 337-41
86. Metra Biosystems. Pyd/Dpd HPLC Calibrator: Assay Literature. Mountain View, CA, USA: Metra Biosystems. 1994: 2
87. Estell R, Simmons PS, Colwell A, Assiri AM, Burritt MF, Russell RG, et al. Nyctohemeral changes in hone turnover assessed by serum hone Gla protein concentration and urinary deoxypyridinoline excretion: effects of growth and ageing. Clin Sci 1992; 83: 375-82
88. McLaren AM, Isdale AH, Whiting PH, Bird HA, Robins SP. Physiological variations in the urinary excretion of pyridinium crosslinks of collagen. Br J Rheumat 1993; 32: 307 12
89. Abbiati G, Bartucci F, Longoni A, Fincato G, Galimberti S, Rigoldi M, et al. Monitoring of free and total urinary pyridinoline and deoxypyridinoline in healthy volunteers: sample relationships between 24-h and fasting early morning urine concentrations. Bone Miner 1993; 21: 9-19
90. Gerrits MI, Thijssen JHH, Herman JM, van Rijn JM. Determination of pyridinoline and deoxypyridinoline in urine, with special attention to retaining their stability. Clin Chem 1995; 41: 571-4
91. Walne AJ, James IT, Perrett D. The stability of pyridinium crosslinks in urine and serum. Clin Chim Acta 1995; 240: 95-7
92. Seibel MJ, Woitge H, Scheidtnave G, Leidigbruekner G, Duncan A, Nicol P, et al. Urinary hydroxypyridinium crosslinks of collagen in population-based screening for overt vertebral osteoporosis: results of a pilot study. J Bone Miner Res 1994; 9: 1433-40
93. Scott PJ, Hurley PJ. Demonstration of individual variation in constancy of 24 hour urinary creatinine excretion. Clin Chim Acta 1968; 21: 411-14
94. Alessio L, Berlin A, Dell'Orto A, Toffoletto F, Ghezzi I. Reliability of urinary creatinine as a parameter used to adjust values of urinary biological indicators. Int Arch Occup Environ Hlth 1985; 55: 99-106
95. Webster J, Garrow JS. Creatinine excretion over 24 hours as a measure of body composition or of completeness of urine collection. Human Nutr-Clin Nutr 1985; 39: 101-6
96. Blumenkrantz N, Asboe-Hansen G. Urinary creatinine is unsuitable as reference value for collagen metabolite output. Clin Biochem 1977; 10: 109-10
97. Heymsfield SB, Arteaga C, McManus C, Smith J, Moffitt S. Measurement of muscle mass in humans: validity of the 24-hour urinary creatinine method. Am J Clin Nutr 1983; 37: 478-94
98. Mitch WE, Collier VU, Walser M. Creatinine metabolism in chronic renal failure. Clin Sci 1980; 58: 327-35
99. Eastell R, Assiri AMA, Blumsohn A, Hannon RA, Peel NFA, Colwell A, et al. The need for several biochemical markers to evaluate bone turnover in older women [Abstract]. J Bone Miner Res 1994; 6: 73
100. Schlemmer A, Johansen JS, Pedersen SA, Jorgensen JO, Hassager C, Christiansen C. The effect of growth hormone (GH) therapy on urinary pyridinoline cross-links in GH-deficient adults. Clin Endocrinol 1991; 35: 471-6
101. Body JJ, Delmas PD. Urinary pyridinium cross-links as markers of hone resorption in tumor-associated hypercalcemia. J Clin Endocrinol Metab 1992; 74: 471 5
102. Lipton A, Deniers L, Daniloff Y, Curley E, Hamilton C, Harvey H, et al. Increased urinary excretion of pyridinium cross-links in cancer patients. Clin Chem 1993; 39: 614-8
103. Spector TD, James IT, Hall GM, Thompson PW, Perrett D, Hart DJ. Increased levels of urinary collagen crosslinks in females with rheumatoid arthritis. Clin Rheumatol 1993; 12: 240-4
104. Seibel MJ, Cosman F, Shen V, Gordon S, Dempster DW, Ratcliffe A, et al. Urinary hydroxypyridinium crosslinks of collagen as markers of bone resorption and estrogen efficacy in postmenopausal osteoporosis. J Bone Miner Res 1993; 8: 881-9
105. Pecherstorfer M, Zimmerroth I, Schilling T, Woitge HW, Schmidt H, Baumgartner G, et al. The diagnostic value of urinary pyridinium cross-links of collagen, serum total alkaline phosphatase. and urinary calcium excretion in neoplastic hone disease. J Clin Endocrinol Metab 1995; 80: 97-103
106. Seibel MJ, Duncan A, Robins SP. Urinary hydroxy-pyridinium crosslinks provide indices of cartilage and bone involvement in arthritic diseases. J Rheumatol 1989; 16: 964-70
107. Ohishi T, Kushida K, Takahashi M, Kawana K, Yagi K, Kawakami K, et al. Urinary bone resorption markers in patients with metabolic bone disorders. Bone 1994; 15: 15-20
108. Wishart JM, Need AG, Horowitz M, Morris HA, Nordin BEC. Effect of age on hone density and bone turnover in men. Clin Endocrinol 1995; 42: 141-6
109. Delmas PD, Gineyts E, Bertholin A, Garnero P, Marchand F. Immunoassay of pyridinoline crosslink excretion in normal adults and in Paget's disease. J Bone Min Res 1993; 8: 643-8
110. Hess B, Casez JP, Takkinen R, Ackermann D, Jaeger P. Relative hypoparathyroidism and calcitriol up-regulation in hypercalciuric calcium renal stone formers - impact of nutrition. Am J Nephrol 1993; 13: 18-26
111. Hopper JL, Seeman E. The hone density of female twins discordant for tobacco use. N Engl J Med 1994; 330: 387-92
112. Lieuw-A-Fa M, Sierra RI, Speeker BL. Carboxyterminal propeptide of human type I collagen and pyridinium crosslinks as markers of bone growth in infants 1 to 18 months of age. J Bone Miner Res 1995; 10: 849-53
113. Blumsohn A, Hannon RA, Wrate R, Barton J, Al-Dehaimi AW, Colwell A, et al. Biochemical markers of hone turnover in girls during puberty. Clin Endocrinol 1994; 40: 663-70
114. Ohishi T, Takahashi M, Kawana K, Aoshima H, Hoshino H, Horiuchi K, et al. Age-related changes of urinary pyridinoline and deoxypyridinoline in Japanese subjects. Clin Invest Med 1993; 16: 319-25
115. Bollen AM, Eyre DR. Bone resorption rates in children monitored by the urinary assay of collagen type I cross-linked peptides. Bone 1994; 15: 31-4
116. Kawana K, Kushida K, Takahashi M, Ohishi T, Denda M, Yamazaki K, et al. The effect of menopause on biochemical markers and ultrasound densitometry in healthy females. Calcif Tissue Int 1994; 55: 420-5
117. Uebelhart D, Schlemmer A, Johansen JS, Gineyts E, Christiansen C, Delmas PD. Effect of menopause and hormone replacement therapy on the urinary excretion of pyridinium cross-links. J Clin Endocrinol Metab 1991; 72: 367-73
118. Hassager C, Colwell A, Assiri AM, Eastell R, Russell RG, Christiansen C. Effect of menopause and hormone replacement therapy on urinary excretion of pyridinium cross-links: a longitudinal and cross-sectional study. Clin Endocrinol 1992; 37: 45-50
119. Schlemmer A, Hassager C, Risteli J, Risteli L, Jensen SB, Christiansen C. Possible variation in bone resorption during the normal menstrual cycle. Acta Endocrinol 1993; 129: 388-92
120. Schlemmer A, Hassager C, Jensen SB, Christiansen C. Marked diurnal variation in urinary excretion of pyridinium cross-links in premenopausal women. J Clin Endocrinol Metab 1992; 74: 476-80
121. Eastell R, Calvo MS, Burritt MF, Offord KP, Russell RG, Riggs BL. Abnormalities in circadian patterns of bone resorption and renal calcium conservation in type I osteoporosis. J Clin Endocrinol Metab 1992; 74: 487-94
122. Fincato G, Bartucci F, Rigoldi M, Abbiati G, Colombo M, Bartolini O, et al. Urinary excretion of pyridinoline and deoxypyridinoline: Circadian rhythm in healthy premenopausal women. J Interdiscipl Cycle Res 1993; 24: 72-8
123. Schlemmer A, Hassager C, Pedersen BJ, Christiansen C. Posture, age, menopause, and osteopenia do not influence the circadian variation in the urinary excretion of pyridinium crosslinks. J Bone Miner Res 1994; 9: 1883-8
124. Pagani P, Panteghini M. Diurnal rhythm in urinary excretion of pyridinium crosslinks. Clin Chem 1994; 40: 952-3
125. Seyedin SM, Kung VT, Daniloff YN, Hesley RP, Gomez B, Nielsen LA, et al. Immunoassay for urinary pyridinoline: the new marker of bone resorption. J Bone Miner Res 1993; 8: 635-41
126. Gertz BJ, Shao P, Hanson DA, Quan H, Harris ST, Genant HK, et al. Monitoring bone resorption in early postmenopausal women by an immunoassay for crosslinked collagen peptides in urine. J Bone Miner Res 1994; 9: 135-42
127. Branca F, Robins SP, Ferro-Luzzi A, Golden MH. Bone turnover in malnourished children. Lancet 1992; 340: 1493-6
128. McLaren AM, Hordon LD, Bird HA, Robins SP. Urinary excretion of pyridinium crosslinks of collagen in patients with osteoporosis and the effects of bone fracture. Ann Rheum Dis 1992; 51: 648-51
129. Robins SP, Siebel MJ, McLaren AM. Collagen markers in urine in human arthritis. In: Maroudas A, Kuettner K. eds. Methods In Cartilage Research. London: Academic Press, 1990: 348-51
130. Isdale A, Helliwell PS. Athletes and osteoarthritis - Is there any relationship? Br J Rheumatol 1991; 30: 67-8
131. Hetland ML, Haarbo J, Christiansen C. Low bone mass and high bone turnover in male long distance runners. J Clin Endocrinol Metab 1993; 77: 770-5
132. Hatori M, Hasegawa A, Adachi H, Shinozaki A, Hayashi R, Okano H, et al. The effects of walking at the anaerobic threshold level on vertebral bone loss in post menopausal women. Calcif Tissue Int 1993; 52: 411-14
133. Morris HA, Wishart JM, Horowitz M, Need AG, Nordin BEC. The reproducibility of bone related biochemical variables in post menopausal women. Ann Clin Biochem 1990; 27: 562-8
134. Blumsohn A, Hannon RA, Al-Dehaimi AW, Eastell R. Short term intraindividual variability of markers of bone turnover in healthy adults [Abstract]. J Bone Miner Res 1994; 9: 132
135. Bonde M, Qvist P, Fledelius C, Riis BJ, Christiansen C. Applications of an enzyme immunoassay for a new marker of bone resorption (crosslaps): follow-up on hormone replacement therapy and osteoporosis risk assessment. J Clin Endocrinol Metab 1995; 80: 864-8
136. Fledelius C, Riis BJ, Overgaard K, Christiansen C. The diagnostic validity of urinary free pyridinolines to identify women at risk of osteoporosis. Calcif Tissue Int 1994; 54: 381-4
137. Eriksen EF, Charles P, Melsen F, Moselkilde L, Risteli L, Risteli J. Serum markers of type I collagen formation and degradation in metabolic bone disease: correlation with bone histomorphometry. J Bone Miner Res 1993; 8: 127-32
138. Hassager C, Jensen LT, Podenphant J, Thomsen K, Christiansen C. The carboxy-terminal pyridinoline crosslinked telopeptide of type I collagen in serum as a marker of bone resorption. The effect of nandrolone decanoate and hormone replacement therapy. Calcif Tissue Int 1994; 54: 30-3
139. Valimaki MJ, Tahtela R, Jones JD, Peterson JM, Riggs BL. Bone resorption in healthy and osteoporotic postmenopausal women: comparison markers for serum carboxy-terminal telopeptide of type I collagen and urinary pyridinium cross-links. Eur J Endocrinol 1994; 131: 258-62
140. Garnero P, Shih WCJ, Gineyts E, Karpf DB, Delmas PD. Comparison of new biochemical markers of bone turnover in late postmenopausal osteoporotic women in response to alendronale treatment. J Clin Endocrinol Metab 1994; 79: 1693-700
141. Blumsohn A, Herrington K, Hannon RA, Shao P, Eyre DR, Eastell R. The effect of calcium supplementation on the circadian rhythm of hone resorption. J Clin Endocrinol Metab 1994; 79: 730-5
142. Rosen HN, Dresner-Pollak R, Moses AC, Rosenblatt M, Zeind AJ, Clemens JD, et al. Specificity of urinary excretion of crosslinked N-telopeptides of type I collagen as a marker of hone collagen turnover. Calcif Tissue Int 1994; 54: 26-9
143. Garnero P, Shih WJ, Gineyts E, Karpf DB, Delmas PD. Assessment of 8 markers of bone turnover in post menopausal (PMP) ostcoporotic women treated with alendronate (ALN). [Abstract]. Second Workshop on Bisphosphonates: From the Laboratory to the Patient, Davos, April 14 16 1994. Geneva: Nichols Institute Diagnostics, 1994
144. Alexandersen P, Schlemmer A, Riis BJ, Christiansen C. Circadian variation in crosslaps - A new marker of type 1 collagen degeneration [Abstract]. J Bone Miner Res 1994; 9: S190
145. Christiansen C, Riis BJ, Rodbro P. Prediction of rapid hone loss in postmenopausal women. Lancet 1987; i: 1605-8
146. Overgaard K, Riis BJ, Bonde M, Christiansen C. A new biochemical marker for the determination of optimum treatment regimen of nasal calcitonin and the effect on fracture rate [Abstract]. J Bone Miner Res 1994; 9: S403