Dissociation of Rab3A by Ca2+/calmodulin from different kinds of synaptosomal membranes

Experimental and Molecular Medicine

Volumn 28, Issue 3, 1996, Pages 147-152

  Park, Jae Bong ^a,b  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b Hallym University   (South Korea)

Author keywords

Calmodulin; Rab3A; Synaptic vesicle; Translocation

Indexed keywords

CALCIUM ION; CALMODULIN; MEMBRANE PROTEIN; RAB PROTEIN; SYNAPTOBREVIN; SYNAPTOPHYSIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DISSOCIATION; FRACTIONATION; MEMBRANE BINDING; NONHUMAN; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; SYNAPTIC MEMBRANE;

ANIMALIA;

EID: 0029827896     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.1038/emm.1996.23     Document Type: Article

References (19)

1. Aepfelbacher, M., Vauti, F., Weber, P.C. and Glomset, J.A. (1994) Spreading of differentiating human monocytes is associated with a major increase in membrane-bound CDC42. Proc. Natl. Acad. Sci. U.S.A. 91: 4263-4267
2. Barrie, A. P., Nicholls, D. G., Sanchez-Prieto, J. and Sihra, T. S. (1991) An ion channellocus for protein kinase C potentiation of transmitter glutamate release from guinea pig cerebrocortical synaptosomes. J. Neurochem. 57: 1398-1404
3. Desnos, C., Clift-O'Grady, L. and Kelly, R. B. (1995) Biogenesis of synaptic vesicles in vitro. J. Cell Biol. 130: 1041-1049
4. Edelman, L., Hanson, P. I., Chapman, E. R. and Jahn, R. (1995) Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine. EMBO J. 14: 224-231
5. Fischer von Mollard, G., Sudhof, T. C. and Jahn, R. (1991) A small GTP-binding protein dissociates from synaptic vesicles during exocytosis. Nature 349: 79-81
6. Fischer von Mollard, G., Stahl, B., Khokhlatchev, A., Sudhof, T. C. and Jahn, R. (1994) Rab3C is a synaptic vesicle protein that dissociates from synaptic vesicles after stimulation of exocytosis. J. Biol. Chem. 269: 10971-10974
7. Gorvel, J. P., Chavrier, P., Zerial, M. and Gruenberg, J. (1991) Rab5 controls early endosome fusion in vitro. Cell 64: 915-925
8. Heuser, J. E. and Reese, T. S. (1973) Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction. J. Cell Biol. 57: 315-344
9. Hoffenberg, S., Sanford, J. C., Liu, S., Daniel, S. D., Tuvin, M., Knoll, B. J., Wessling-Resnick, M. and Dicky, B. F. (1995) Biochemical and functional characterization of a recombinant GTPase, Rab5, and two of its mutants. J. Biol. Chem. 270: 5048-5056
10. Huttner, W. B., Greengard, P. and De Camilli, P. (1983) Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicl preparation. J. Cell Biol. 96: 1374-1388
11. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227: 680-685
12. Li, G. and Stahl, P. D. (1993) Structure-function relatioship of the small GTPase rab5. J. Biol. Chem. 268: 24475-24480
13. Leube, R. E., Kaiser, P., Seiter, A., Zimbelmann, R., Franke, W. W., Rehm, H., Kn, P., Prior, P., Betz, H., Reinke, H., Beyreuther, K. and Wiedenmann, B. (1985) Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA. EMBO J. 6: 3261-3268
14. Miller, S. G. and Moore, H.-P. (1990) Regulated secretion. Curr. Opin. Cell Biol. 2: 642-647
15. 2+/calmodulin causes Rab3A to dissociate from synaptosomal membranes. submitted for publication.
16. Sasaki, T., Kikuchi, A., Araki, S., Hata, Y., Isomura, M., Kuroda, S. and Takai, Y. (1990) Purification and characterization from bovine brain cytosol of a protein that inhibits the dissociation of GDP from and the subsequent binding of GTP to ras p21-like GTP-binding protein. J. Biol. Chem. 265: 2333-2337
17. Söllner, T., Whiteheart, S. W., Brunner, M., Erdjument-Bromage, H., Germanos, S., Tempst, P. and Rothman, J. E. (1993) SNAP receptors implicated in vesicle targeting and fusion. Nature 362: 318-324
18. Südhof, T. C. and Jahn, R. (1991) Proteins of synaptic vesicles involved in exocytosis and membrane recycling. Neuron 6: 665-677
19. Ullrich, O., Stenmark, H., Alexandrov, K., Huber, L. A., Kaibuchi, K., Sasaki, T., Takai, Y. and Zerial, M. (1993) Rab GDP dissociation inhibitor as a general regulator for the membrane association of Rab proteins. J. Biol. Chem. 268, 18143-18150