Chimeras of parvalbumin and oncomodulin involving exchange of the complete CD site show that the Ca2+/Mg2+ specificity is an intrinsic property of the site

European Journal of Biochemistry

Volumn 242, Issue 2, 1996, Pages 256-263

  Durussel, Isabelle ^a   Pauls, Thomas L ^b   Cox, Jos A ^a,d   Berchtold, Martin W ^c  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b UNIVERSITY OF FRIBOURG   (Switzerland)

^c UNIVERSITY OF ZURICH   (Switzerland)

^d Department of Biochemistry ^*  (Switzerland)

Author keywords

Calcium binding; Magnesium binding; Mutagenesis; Oncomodulin; Parvalbumin

Indexed keywords

CALCIUM; CHIMERIC PROTEIN; MAGNESIUM; MUTANT PROTEIN; ONCOMODULIN; PARVALBUMIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CONTROLLED STUDY; GENE CONSTRUCT; GENE EXPRESSION; MASS SPECTROMETRY; METAL BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; SPECTROFLUOROMETRY;

EID: 0029827616     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0256r.x     Document Type: Article

References (23)

1. Blancuzzi, Y., Padilla, A., Parello, J. & Cavé, A. (1993) Biochemistry 32, 1302-1309.
2. Clayshulte, T., Taylor, D. F. & Henzl, M. T. (1990) J. Biol. Chem. 265, 1800-1805.
3. Colowick, S. P. & Womack, F. C. (1969) J. Biol. Chem. 244, 774-777.
4. Cox, J. A., Milos, M. & MacManus, J. P. (1990) J. Biol. Chem. 265, 6633-6637.
5. Declerq, J. P., Tinant, B., Parello, J. & Rambaud, J. (1991) J. Mol. Biol. 220, 1017-1039.
6. Durussel, I., Rhyner, J. A., Strehler, E. E. & Cox, J. A. (1993) Biochemistry 32, 6089-6094.
7. George, S. E., Su, Z., Fan, D. & Means, A. R. (1993) J. Biol. Chem. 268, 25 213-25 220.
8. Hapak, R. C., Lammers, P. J., Palmisano, W. A., Birnbaum, E. R. & Henzl, M. T. (1989) J. Biol. Chem. 264, 18 751-18 760.
9. Hutnik, C. M. L., MacManus, J. P., Banville, D. & Szabo, A. G. (1990a) J. Biol. Chem. 265, 11 456-11 464.
10. Hutnik, C. M. L., McManus, J. P. & Szabo, A. G. (1990b) Biochemistry 29, 7318-7328.
11. Kauffman, J. F., Hapak, R. C. & Henzl, M. T. (1995) Biochemistry 34, 991-1000.
12. MacManus, J. P., Hutnik, C. M. L., Sykes, B. D., Szabo, A. G., Williams, T. C. & Banville, D. (1989) J. Biol. Chem. 264, 3470-3477.
13. Palmisano, W. A., Trevino, C. L. & Henzl, M. T. (1990) J. Biol. Chem. 265, 14 450-14 456.
14. Pauls, T. L. & Berchthold, M. W. (1993) Methods Enzymol. 217, 102-122.
15. Pauls, T. L., Durussel, I., Cox, J. A., Clark, I. D., Szabo, A. G., Gagné, S. M., Sykes, B. D. & Berchthold, M. W. (1993) J. Biol. Chem. 268, 20 897-20 903.
16. Pauls, T. L., Durussel, I., Berchtold, M. W. & Cox, J. A. (1994) Biochemistry 33, 10 393-10 400.
17. Pauls, T. L., Durussel, I., Clark, I. D., Szabo, A. G., Berchtold, M. W. & Cox, J. A. (1996) Eur. J. Biochem. 242, 249-255.
18. Permyakov, E. A., Medvedkin, V. N., Mitin, Y. V. & Kretsinger, R. H. (1991) Biochim. Biophys. Acta 1076, 67-70.
19. Riddles, P. W., Blakeley, R. L. & Zerner, B. (1983) Methods Enzymol. 91, 49-60.
20. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) Proc. Natl Acad. Sci. USA 74, 5463-5467.
21. Trevino, C. L., Boschi, J. M. & Henzl, M. T. (1991 ) J. Biol. Chem. 266, 11 301-11 308.
22. Williams, T. C., Corson, D. C., Oikawa, K., McCubbin, W. D., Kay, C. M. & Sykes, B. D. (1986) Biochemistry 25, 1835-1846.
23. Wnuk, W., Cox, J. A. & Stein, E. A. (1981) in Calcium & cell function (Cheung, W. Y., ed.) vol. 2, pp. 243-278, Academic Press, New York.