Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding

Molecular Reproduction and Development

Volumn 45, Issue 2, 1996, Pages 193-203

  Hou, Sheng Tao ^a   Ma, Alicia ^b   Jones, Roy ^b   Hall, Len ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b INSTITUTE OF FOOD RESEARCH   (United Kingdom)

Author keywords

Bifunctional protein; Fertilization; Hyaluronidase activity; Zona binding protein

Indexed keywords

HYALURONIDASE; SPERM ANTIGEN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; ENZYME ACTIVITY; FERTILIZATION; MALE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RAT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, SURFACE; BASE SEQUENCE; BINDING SITES; CELL ADHESION MOLECULES; CLONING, MOLECULAR; FEMALE; GLYCOPROTEINS; HUMANS; HYALURONOGLUCOSAMINIDASE; MALE; MICE; MOLECULAR SEQUENCE DATA; PROTEOCHONDROITIN SULFATES; RABBITS; RATS; RATS, WISTAR; SEQUENCE ALIGNMENT; SPERM-OVUM INTERACTIONS; SPERMATOZOA;

ANIMALIA; APIS MELLIFERA; APOIDEA; BACTERIA (MICROORGANISMS); CAVIA PORCELLUS; ORYCTOLAGUS CUNICULUS; SUS SCROFA;

EID: 0029824142     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199610)45:2<193::AID-MRD12>3.0.CO;2-2     Document Type: Article

References (37)

1. Bordier C (1981): Phase-separation of integral membrane proteins in Triton X-114 solution. J Biol Chem 256:1604-1607.
2. Claros MG, von Heijne G (1994): TopPred II: An improved software for membrane protein structure predictions. Comput Appl Biosci 10:685-686.
3. Davies A, Simmons DL, Hale G, Harrison RA, Tighe H, Lachmann PJ, Waldmann H (1989): CD59, an LY6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. J Exp Med 170:637-654.
4. Ferguson MAJ (1988): Cell-surface anchoring of proteins via glycosylphosphatidylinositol structures. Annu Rev Biochem 57:285-320.
5. Gacesa P, Civill ND, Harrison RAP (1994): PH-20 and sperm hyaluronidase - A conceptual conundrum in mammalian fertilization. Biochem J 303:335-336.
6. Gaunt SJ, Brown CR, Jones R (1983): Identification of mobile and fixed antigens on the plasma membrane of rat spermatozoa using monoclonal antibodies. Exp Cell Res 144:275-284.
7. Giulian GG, Moss RL, Greaser M (1983): Improved methodology for analysis and quantitation of proteins on one-dimensional silver-stained slab gels. Anal Biochem 129:277-287.
8. Gmachl M, Kreil G (1993): Bee venom hyaluronidase is homologous to a membrane protein of mammalian sperm. Proc Natl Acad Sci USA 90:3569-3573.
9. Gmachl M, Sagan S, Ketter S, Kreil G (1993): The human sperm protein PH-20 has hyaluronidase activity. FEBS Lett 336:545-548.
10. Harrison RAP, Gaunt SJ (1988): Multiple forms of ram and bull sperm hyaluronidase revealed by using monoclonal-antibodies. J Reprod Fertil 82:777-785.
11. Hope TJ, Zhu L, Parslow TG, Yang B, Stern M, Turley EA, Stern R (1993): Partial sequence of porcine liver hyaluronidase: Identification of a hemopexin and two putative HA-binding domains. J Cell Biochem [Suppl] 17:175.
12. Hotez PJ, Narasimhan S, Haggerty J, Milstone L, Bhopale V, Schad GA, Richards FF (1992): Hyaluronidase from infective ancylostoma hookworm larvae and its possible function as a virulence factor in tissue invasion and in cutaneous larva migrans. Infect Immun 60:1018-1023.
13. Humphreis SE, Whittal R, Minty A, Buckingham M, Williamson R (1981): There are approximately 20 actin genes in the human genome. Nucleic Acids Res 9:4895-4908.
14. Jones R (1986): Preparation of a plasma membrane-rich fraction from rat spermatozoa. J Reprod Fertil 77:435-449.
15. Jones R (1990): Identification and functions of mammalian sperm-egg recognition molecules during fertilization. J Reprod Fertil [Suppl] 42:89-105.
16. Jones R, Shalgi R, Hoyland J, Phillips DM (1990): Topographical rearrangement of a plasma membrane antigen during capacitation of rat spermatozoa in vitro. Dev Biol 139:349-362.
17. Jones R, Ma A, Hou ST, Shalgi R, Hall L (1996): Testicular biosynthesis and epididymal endoproteolytic processing of rat sperm surface antigen 2B1. J Cell Sci (in press).
18. Kyte J, Doolittle RF (1982): A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105-132.
19. Laemmli UK (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
20. Lathrop WF, Carmichael EP, Myles DG, Primakoff P (1990): cDNA cloning reveals the molecular structure of a sperm surface protein, PH-20, involved in sperm-egg adhesion and the wide distribution of its gene among mammals. J Cell Biol 111:2939-2949.
21. Lin Y, Kimmel LH, Myles DG, Primakoff P (1993): Molecular cloning of the human and monkey sperm surface protein PH-20. Proc Natl Acad Sci USA 90:10071-10075.
22. Lin Y, Mahan K, Lathrop WF, Myles DG, Primakoff P (1994): A hyaluronidase activity of the sperm plasma membrane protein PH-20 enables sperm to penetrate the cumulus cell layer surrounding the egg. J Cell Biol 125:1157-1163.
23. Low MG (1989): Glycosyl-phosphatidylinositol: A versatile anchor for all surface proteins. FASEB J 3:1600-1608.
24. Low MG, Ferguson MAJ, Futerman AH, Silman I (1986): Covalently attached phosphatidylinositol as a hydrophobic anchor for membrane-proteins. TIBS 11:212-215.
25. Lu G, Kochoumian L, King TP (1995): Sequence identity and antigenic cross-reactivity of white hornet venom allergen, also a hyaluronidase, with other proteins. J Biol Chem 270:4457-4465.
26. Myles DG, Primakoff P (1984): Localized surface antigens of guinea-pig sperm migrate to new regions prior to fertilization. J Cell Biol 99:1634-1641.
27. O'Farrell P (1974): High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007-4021.
28. Perry ACF, Gichuhi PM, Jones R, Hall L (1995): Cloning and analysis of monkey fertilin reveals novel a subunit isoforms. Biochem J 307:843-850.
29. Phelps BM, Primakoff P, Koppel DE, Low MG, Myles DG (1988): Restricted lateral diffusion of PH-20; a PI-anchored sperm membrane protein. Science 240:1780-1782.
30. Phelps BM, Koppel DE, Primakoff P, Myles DG (1990): Evidence that proteolysis of the surface is an initial step in the mechanism of formation of sperm cell surface domains. J Cell Biol 111:1839-1847.
31. Primakoff P, Lathrop W, Woolman L, Cowan A, Myles DG (1988): Fully effective contraception in male and female guinea-pigs immunised with the sperm protein PH-20. Nature 335:543-546.
32. Shalgi R, Matityahu A, Gaunt SJ, Jones R (1990): Identification of antigens on rat spermatozoa with a potential role in fertilization. Mol Reprod Dev 25:286-296.
33. Talbot P (1984): Hyaluronidase dissolves a component in the hamster zona pellucida. J Exp Zool 229:309-316.
34. Twigg AJ, Sherratt D (1980): Trans-complementable copy-number mutants of plasmid CoE1. Nature 283:216-218.
35. Yanagimachi R (1994): Mammalian fertilization. In Z Knobil, ID Neill (eds): "The Physiology of Reproduction," 2nd edition. New York: Raven Press, pp 189-317.
36. Yang B, Yang BL, Savani RC, Turley EA (1994): Identification of a common hyaluron binding motif in the hyaluron binding proteins RHAMM, CD44 and link protein. EMBO J 13:286-296.
37. Yang CH, Srivastava PN (1974): Separation and properties of hyaluronidase from ram sperm acrosomes. J Reprod Fertil 37:17-25.