Okadaic acid disrupts Golgi structure and impairs enzyme synthesis and secretion in the rat pancreas

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 270, Issue 6 33-6, 1996, Pages

  Waschulewski, Ingo H ^b   Kruse, Marie Luise ^b   Agricola, Brigitte ^b   Kern, Horst F ^b   Schmidt, Wolfgang E ^a  

^a UNIVERSITY OF KIEL   (Germany)

^b NONE

Author keywords

exocrine pancreas; FOY 305; Golgi complex; pancreatic enzyme secretion; serine threonine phosphatases

Indexed keywords

CAMOSTAT MESILATE; OKADAIC ACID; PHOSPHATASE; PROTEIN SERINE THREONINE KINASE;

ACINAR CELL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL ULTRASTRUCTURE; ELECTRON MICROSCOPY; ENZYME RELEASE; GOLGI COMPLEX; MALE; NONHUMAN; PANCREAS; PRIORITY JOURNAL; PROTEIN SECRETION; PROTEIN SYNTHESIS; PROTEIN SYNTHESIS INHIBITION; RAT;

EID: 0029823784     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1996.270.6.g939     Document Type: Article

References (33)

1. Azhar, S., L. Tsai, L. Cao, and E. Reaven. Okadaic acid leads to Golgi disruption and loss of VLDL secretion in hepatocytes (Abstract). J. Cell Biol. 119, 866, 1992.
2. Bani, D., F. Filipponi, I. Magnani, D. Houssin, and P. Romagnoli. Morphological changes in rat pancreatic acinar cells induced by long-term treatment with cyclosporine and their reversal after withdrawal. Transplant 50: 830-834, 1990.
3. Burnham, D. B., P. Munowitz, S. R. Hootmann, and J. A. Williams. Regulation of protein phosphorylation in pancreatic acini. Biochem. J. 235: 125-131, 1986.
4. Burnham, D. B., C. K. Sung, P. Munowitz, and J. A. Williams. Regulation of protein phosphorylation in pancreatic acini by cyclic AMP-mediated secretagogues: interaction with carbamylcholine. Biochim. Biophys. Acta 169: 33-39, 1988.
5. Burnham, D. B., and J. A. Williams. Effects of carbachol, cholecystokinin and insulin on protein phosphorylation in isolated pancreatic acini. J. Cell Biol. 257: 10523-10528, 1982.
6. Cohen, P. The role of protein phosphorylation in neural and hormonal control of cellular activity. Nature Lond. 296: 613-620, 1982.
7. Cohen, P., C. F. B. Holmes, and Y. Tsukitani. Okadaic acid: a new probe for the study of cellular regulation. Trends Biol. Sci. 15: 98-102, 1990.
8. Doi, R., K. Inoue, P. Chowdhury, H. Kaj, and P. L. Rayford. Structural and functional changes of exocrine pancreas induced by FK 506 in rats. Gastroenterology 104: 1153-1164, 1993.
9. Doi, R., A. Tangoku, K. Inoue, P. Chowdhury, and P. L. Rayford. Effects of FK 506 on exocrine pancreas in rats. Pancreas 7: 197-204, 1992.
10. Estevez, M. D., M. R. Vieytes, M. C. Louzao, and L. M. Botana. Effect of okadaic acid on immunologic and non-immunologic histamine release in rat mast cells. Biochem. Pharmacol. 47: 591-593, 1991.
11. Göke, B., J. A. Williams, M. J. Wishart, and R. C. De Lisle. Low molecular mass GTP-binding proteins in subcellular fractions of the pancreas: regulated phosphoryl G proteins. Am. J. Physiol. 262 (Cell Physiol. 31): C493-C500, 1992.
12. 2+/calmodulin-dependent protein phosphatase and secretion in pancreatic acinar cells. J. Biol. Chem. 269: 15111-15117, 1994.
13. Haystead, T. A. J., A. T. R. Sim, D. Carling, R. C. Honnor, Y. Tsukitani, P. Cohen, and D. G. Hardie. Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism. Nature Lond. 337: 78-81, 1989.
14. Helmchen, U., W. E. Schmidt, E. G. Siegel, and W. Creutzfeldt. Morphological and functional changes of pancreatic B cells in cyclosporine A-treated rats. Diabetologia 27. 416-418, 1984.
15. Ingebritsen, T. S., and P. Cohen. Protein phosphatases: properties and role in cellular regulation. Science Wash. DC 221: 331-338, 1983.
16. Kruse-Jarres, J. D., C. Kaiser, J. C. Hafkenscheid, W. Hohenwallner, W. Stein, J. Bohner, G. Klein, W. Poppe, and E. Rauscher. Evaluation of a new alpha-amylase assay using 4,6-ethylidene-(G/)-1 4-nitrophenyl-(G1)-alpha-D-maltohepatoside as substrate. J. Clin. Chem. Clin. Biochem. 27: 103-107, 1989.
17. Lucocq, J., G. Warren, and J. Pryde. Okadaic acid induces Golgi apparatus fragmentation and arrest of intracellular transport. J. Cell Sci. 100: 753-759, 1991.
18. MacLean, C. M., S. J. Mariniak, D. V. Hall, and J. M. Edwardson. Involvement of a phosphoprotein on the zymogen granule membrane in the control of regulated exocytosis in the exocrine pancreas. J. Cell Sci 106: 663-670, 1993.
19. Mans, R. J., and G. D. Novelli. Measurement of the incorporation of radioactive amino acids into protein by a filter paper disc method. Arch. Biochem. Biophys. 94: 48-53, 1961.
20. Mason, J. Pharmacology of cyclosporine (Sandimmune): pathophysiology and toxicology of cyclosporine in humans and animals. Pharmacol. Rev. 42: 423-434, 1989.
21. Meyer-Alber, A., I. Fetz, I. H. Waschulewski, M. Höcker, U. R. Fölsch, and W. E. Schmidt. Calyculin A, okadaic acid and W-7 interfere with a distal step in pancreatic acinar signal transduction. Biochem. Biophys. Res. Commun. 201: 1470-1476, 1994.
22. Meyer-Alber, A., M. Höcker, I. Fetz, H. Fornefeld, I. H. Waschulewski, U. R. Fölsch, and W. E. Schmidt. Differential inhibitory effects of serine/threonine phosphatase inhibitors and a calmodulin antagonist on calcium/phosphoinositol- and cAMP-mediated pancreatic amylase secretion. Scand. J. Gastroenterol. 30: 384-391, 1995.
23. Palade, G. E. Intracellular aspects of the process of protein secretion. Science Wash. DC 189: 347-358, 1975.
24. Rausch, U., H. Weidenbach, G. Adler, and H. F. Kern. Stimulation of pancreatic secretory process in the rat by low molecular weight proteinase inhibitor. II. Regulation of total protein and individual enzyme biosynthesis. Cell Tiss. Res. 249: 63-67, 1987.
25. Reaven, E., L. Tsai, B. Maffe, and S. Azhar. Effect of okadaic acid on hepatocyte structure and function. Cell Mol. Biol. Res. 39: 275-288, 1993.
26. Richards, G. M. Modifications of diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal. Biochem. 57: 369-376, 1974.
27. Scheele, G. A., and G. E. Palade. Studies on the pancreas of the guinea pig. Parallel discharge of exocrine enzyme activities. J. Biol. Chem. 250: 2660-2670, 1975.
28. Schmidt, W. E., A. Meyer-Alber, I. H. Waschulewski, I. Fetz, M. Höcker, H. F. Kern, and U. R. Fölsch. Serine/threonine phosphatases play a role in stimulus-secretion coupling in pancreatic acinar cells (Abstract). Z. Gastroenterol. 32: 226, 1994.
29. Vasiloudes, P., N. Paul, J. Tooze, and H. F. Kern. Acceleration of asynchronous enzyme transport in the rat exocrine pancreas following hormonal stimulation in vivo. Eur. J. Cell Biol. 54: 27-37, 1991.
30. Wagner, A. C. C., M. J. Wishart, D. I. Yule, and J. A. Williams. Effects of okadaic acid indicate a role for dephosphorylation in pancreatic stimulus secretion coupling. Am. J. Physiol. 263 (Cell Physiol. 32): C1172-C1180, 1992.
31. Waschulewski, I. H., D. V. Hall, H. E. Kern, and J. M. Edwardson. Effects of the immunosuppressants cyclosporin A and FK 506 on exocytosis in the rat exocrine pancreas in vitro. Br. J. Pharmacol. 108: 892-900, 1993.
32. Williams, J. A., and D. I. Yule. Stimulus-secretion coupling in pancreatic acinar cells. In: The Pancreas - Biology, Pathobiology and Disease, edited by V. L. W. Go, E. P. Dimagno, J. D. Gardner, E. Lebenthal, H. A. Reber, and G. A. Scheele. New York: Raven, 1993, p. 167-191.
33. Yanagihara, N., Y. Toyohira, Y. Koda, A. Wada, and F. Izumi. Inhibitory effect of okadaic acid on carbachol evoked secretion of catecholamines in cultured bovine adrenal medullary cells. Biochem. Biophys. Res. Commun. 174: 77-83, 1991.