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10244271000
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note
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2+-CO verdoheme (614 nm).
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16
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10244245263
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note
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Iron(III) porphyrins 3 and 4 were combined with hHO-1 in a 2:1 heme to enzyme ratio, and the mixture was purified over a BioRad HTP column to give a 1:1 complex in 100 mM potassium phosphate buffer (pH 7.4).
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18
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10244259478
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note
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Semipreparative reverse phase (C18) HPLC was done with 50:50 v/v acetone/0.1% aqueous formic acid at a flow rate of 3.0 mL/min. Mass spectra were obtained by (+)LSIMS using a 1:1 (1% trifluoroacetic acid) glycerol/thioglycerol matrix.
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0001428270
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Bag, N.3
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