Limitation of enzyme-conjugated streptavidin-biotin system in the detection of eukaryotic proteins by immunoblot analysis

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 115, Issue 1, 1996, Pages 57-61

  Kim, Seong Hun ^b   Suh, Yoo Hun ^a  

^a Seoul National University   (South Korea)

^b NONE

Author keywords

avidin; biotin; carboxylase; immunoblot; streptavidin

Indexed keywords

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IMMUNOBLOTTING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; TECHNIQUE;

ANIMALIA; EUKARYOTA;

EID: 0029823594     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/0305-0491(96)00084-3     Document Type: Article

References (17)

1. Bayer, C.A.; Wilchek, M. The use of the avidin-biotin complex as a tool in molecular biology. Methods Biochem. Anal. 26:1-45;1980.
2. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254;1976.
3. De Lange, R.J.; Huang, T.S. Egg white avidin. J. Biol. Chem. 246:696-709;1971.
4. Diamandis, E.P.; Christopoulos, T.K. The biotin-(strept)avidin system: principles and applications in biotechnology, Clin. Chem. 37:625-636;1991.
5. Diamandis, E.P.; Morton, R.C. Time-resolved fluorescence using a europium chelate of 4,7-bis-(chlorosulfophenyl)-, 10-phenanthroline-2,9-dicarboxylic acid (BCPDA). Labeling procedures and applications in immunoassays. J. Immunol. Methods 112:43-52;1988.
6. Graham, J. Isolation of subcellular organelles and membranes. In: Rickwood, D. (ed). Centrifugation: A Practical Approach. IRL Press; 1975: pp. 161-173.
7. Green, N.M. Avidin. Adv. Protein Chem. 29:85-133;1975.
8. Haneji, T; Koide, S.S. Transblot identification of biotincontaining proteins in rat liver. Anal. Biochem. 177:57-61; 1989.
9. Haneji, T.; Toyama, Y.; Nagano, T. Detection of endogenous avidin-binding proteins in rat liver cells by transblot and electron microscopy. J. Electron Microsc. Tokyo 42:231-235; 1993.
10. Kirkeby, S.; Moe, D.; Bog-Hansen, T.C.; van-Noorden, C.J. Biotin carboxylases in mitochondria and the cytosol from skeletal and cardiac muscle as detected by avidin binding. Histochemistry 100:415-421;1993.
11. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680-685; 1970.
12. Towbin H.; Staehelin, T.; Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354;1979.
13. Wakil, S.J.; Titchener, E.B.; Gibson, D.M. Evidence for the participation of biotin in the enzymic synthesis of fatty acids. Biochem. Biophys. Acta. 29:225-226;1958.
14. Wang, D.; Waye, M.M.Y.; Taricani, M.; Buckingham, K.; Sandham, H.J. Biotin-containing protein as a cause of false positive clones in gene probing with streptavidin/biotin. Bio-Techniques 14:209-211;1991.
15. Wilchek, M.; Bayer, E.A. The avidin-biotin complex in bioanalytical applications. Anal. Biochem. 171:1-32;1988.
16. Wilchek, M.; Bayer, E.A. Methods in Enyzmology, Vol. 184. New York: Academic Press; 1990: pp. 1-746.
17. Wood, G.S.; Warnke, R. Suppression of endogenous avidinbinding activity in tissues and its relevance to biotin-avidin detection system. J. Histochem. Cytochem. 29:1196-1204.