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Volumn 273, Issue 5278, 1996, Pages 1109-1112

Role of postreplicative DNA mismatch repair in the cytotoxic action of thioguanine

Author keywords

[No Author keywords available]

Indexed keywords

AZATHIOPRINE; MERCAPTOPURINE; METHYLNITROSOUREA; TIOGUANINE;

EID: 0029822592     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5278.1109     Document Type: Article
Times cited : (367)

References (31)
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    • note
    • 3H]methylthiodeoxy-guanosine was estimated with the assumption that the specific radioactivity of the SAM pool would be the same as that of the methionine in the medium.
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    • 6-methylguanine) in 100 μl of 50 mM tris-HCI (pH 7.6) buffer containing 10 mM dithiothreitol and bovine serum albumin (200 μg/mol). At intervals, samples were removed and the repaired demethylated DNA was separated from the substrate DNA by chromatography on MonoQ (11). The amount of repaired and remaining unrepaired DNA was determined by scintillation counting.
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    • 32P]-labeled DNA were found by autoradiography and the corresponding bands were excised and quantified by scintillation counting.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.