Na pump current can be separated into ouabain-sensitive and -insensitive components in single rat ventricular myocytes

Japanese Journal of Physiology

Volumn 46, Issue 3, 1996, Pages 215-223

  Ishizuka, Nobuhiko ^b   Fielding, Alexander J ^a   Berlin, Joshua R ^a  

^a GRADUATE HOSPITAL   (United States)

^b CHUGAI PHARMACEUTICAL CO LTD   (Japan)

Author keywords

Heart; Isoforms; Na,K ATPase; Ouabain; Rat; Vanadate

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ISOENZYME; OUABAIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DRUG SENSITIVITY; ENZYME ACTIVITY; ENZYME KINETICS; HEART MUSCLE CELL; HEART VENTRICLE; MALE; NONHUMAN; PATCH CLAMP; RAT; SODIUM TRANSPORT;

EID: 0029822314     PISSN: 0021521X     EISSN: None     Source Type: Journal    
DOI: 10.2170/jjphysiol.46.215     Document Type: Article

References (35)

1. De Weer P, Gadsby DC, and Rakowski RF. Voltage dependence of the Na-K pump. Annu Rev Physiol 50 225-241, 1988
2. Eisner DA, Lederer WJ, and Vaughan-Jones RD: The quantitative relationship between twitch tension and intracellular sodium activity in sheep cardiac Purkinje fibres J Physiol (Lond) 355: 251-266, 1984
3. +-ATPase In: The Sodium Pump: Structure, Mechanism and Regulation, ed. Kaplan JH and De Weer P, Rockefeller University Press, New York, pp 63-76, 1991
4. +-ATPase. Biochim Biophys Acta 988: 185-220, 1989
5. Charlemagne D, Mayoux E, Poyard M, Oliviero P, and Geering K: Identification of two isoforms of the catalytic subunit of Na,K-ATPase in myocytes from adult rat heart. J Biol Chem 262: 8941-8943, 1987
6. +-ATPases with different affinities for ouabain Developmental changes in immunologically different catalytic subunits. Proc Natl Acad Sci USA 84: 8404-8407, 1987
7. Akera T, Ng Y-C, Hadley R, Katano Y, and Brody TM: High affinity and low affinity ouabain binding sites in the rat heart Eur J Pharmacol 132: 137-146, 1986
8. Grupp IL, Grupp G, and Schwartz A: Digitalis receptor desensitization in rat ventricle. Ouabain produces two inotropic effects. Life Sci 29: 2789-2794, 1981
9. Berlin JR, Fielding AJ, and Ishizuka N: Identification of low and high affinity ouabain-sensitive Na pump current in voltage-clamped rat cardiac myocytes. Proc NY Acad Sci 671: 440-442, 1992
10. 2+ indicator, furaptra. Biophys J 64: 1331-1343, 1993.
11. Cannell MB and Lederer WJ: A novel experimental chamber for single-cell voltage-clamp and patch-clamp applications with low electrical noise and excellent temperature and flow control. Pflügers Arch 406: 536-539, 1986
12. Gadsby DC and Nakao M. Steady-state current-voltage relationship of the Na/K pump in guinea pig ventricular myocytes. J Gen Physiol 94: 511-537, 1989
13. + pump current-voltage relationship of isolated rat and guinea-pig heart cells. J Physiol (Lond) 481: 279-291, 1994
14. o activation of the Na-K pump in adult rat cardiac myocytes. Am J Physiol 266: C37-C41, 1994
15. Nakao M and Gadsby DC: [Na] and [K] dependence of the Na/K pump current-voltage relationship in guinea pig ventricular myocytes. J Gen Physiol 94: 539-565, 1989
16. 1 on activity and voltage dependence of the Na/K pump in adult rat cardiac myocytes. J Membr Biol 135: 39-47, 1993
17. + pump. Biochim Biophys Acta 727: 336-350, 1983
18. Hilgemann DW, Nagel GA, and Gadsby DC. Na/K pump current in giant membrane patches excised from ventricular myocytes, In: The Sodium Pump: Structure, Mechanism and Regulation, ed. Kaplan JH and De Weer P, Rockefeller University Press, New York, pp 543-547, 1991
19. Fielding AJ, Ishizuka N, and Berlin JR. Kinetics of Na pump inhibition by vanadate are simulated by a Post-Albers model of the Na,K-ATPase. Biophys J 64: A332, 1993 (abstract)
20. Gadsby DC, Kimura J, and Noma A: Voltage dependence of Na/K pump current in isolated heart cells. Nature 315: 63-65, 1985
21. Glitsch HG, Krahn T, and Pusch H: The dependence of sodium pump current on internal Na concentration and membrane potential in cardioballs from sheep Purkinje fibres. Pflügers Arch 414: 52-58, 1989
22. + in mammalian cardiac myocytes Am J Physiol 259: H488-H496, 1990
23. + pump current in isolated rabbit ventricular myocytes using the whole-cell voltage-clamp technique. Circ Res 72: 91-101, 1993
24. Mogul DJ, Singer DH, and Ten Eick RE: Ionic diffusion in voltage-clamped isolated cardiac myocytes Biophys J 56: 565-577, 1989
25. + concentration in internally perfused cardiac cells. Biochim Biophys Acta 1065: 269-271, 1991
26. Wendt-Gallitelli M-F, Voigt T, and Isenberg G: Microheterogeneity of subsarcolemmal sodium gradients. Electron probe microanalysis in guinea-pig ventricular myocytes. J Physiol (Lond) 472: 33-44, 1993
27. +)-ATPase activity and force of contraction in rat heart. Biochem Pharmacol 29: 3219-3229, 1980
28. Lucchesi PA and Sweadner KJ: Postnatal changes in Na,K-ATPase isoform expression in rat cardiac ventricle. J Biol Chem 266: 9327-9331, 1991
29. Sweadner KJ: Enzymatic properties of separated isozymes of the Na,K-ATPase. J Biol Chem 260: 11508-11513, 1985
30. Urayama O and Nakao M: Organ specificity of rat sodium- and potassium-activated adenosine triphosphatase. J Biochem 86: 1371-1381, 1979
31. Gao J, Mathias RT, Cohen IS, and Baldo GJ: Two functionally different Na/K pumps in guinea pig ventricular myocytes. J Gen Physiol 106: 995-1030, 1995
32. +-ATPaSe is dependent on isozyme and environment of the pump. Am J Physiol 258: C803-C811, 1990
33. Jewell EA and Lingrel JB: Comparison of the substrate dependence properties of the rat Na,K-ATPase α1, α2, and α3 isoforms expressed in HeLa cells. J Biol Chem 266: 16925-16930, 1991
34. +)-ATPase. J Biol Chem 260: 10075-10080, 1985
35. +) pump. J Biol Chem 266: 15817-15823, 1991