Importance of liposome complexing volume in transfection optimization

BioTechniques

Volumn 21, Issue 5, 1996, Pages 792-798

  Staggs, David R ^a   Burton, Douglas W ^a,b   Deftos, L J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b VA SAN DIEGO HEALTHCARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; DIMETHYLDIOCTADECYLAMMONIUM BROMIDE; DIOLEOYLPHOSPHATIDYLETHANOLAMINE; LIPOSOME; PLASMID DNA;

ANIMAL CELL; ARTICLE; CANCER CELL CULTURE; CELL LINE; CELL LYSATE; COMPLEX FORMATION; CULTURE MEDIUM; DNA PURIFICATION; DNA TRANSFECTION; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE EXPRESSION; HUMAN; HUMAN CELL; NONHUMAN; PARTICLE SIZE; PROTEIN EXPRESSION; REPORTER GENE;

ANIMALIA;

EID: 0029821363     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215bm08     Document Type: Article

References (7)

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2. Cepko, C. 1992. Xgal staining of cultured cells, p. 9.11.9-9.11.12. In F.A. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.A. Smith, J.G. Seidman and K. Struhl (Eds.), Current Protocols in Molecular Biology. Greene Publishing Associates and John Wiley Interscience, New York.
3. Eustice, D.C., P.A. Feldman, A.M. Colberg-Poley, R.M. Buckery and R.H. Neubauer. 1991. A sensitive method for the detection of β-galactosidase in transfected mammalian cells. BioTechniques 11:739-743.
4. Felgner, P.L., T.R. Gadek, M. Holm, R. Roman, H.W. Chan, M. Wenz, J.P. Northrop, G.M. Ringold and G.A. Danielsen. 1987. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA 84:7413-7417.
5. Felgner, P.L. and G.M. Ringold. 1989. Cationic liposome-mediated transfection. Nature 337:387-388.
6. Rose, J.K., L. Buonocore and M.A. Whitt. 1991. A new cationic liposome reagent mediating nearly quantitative transfection of animal cells. BioTechniques 10:520-525.
7. Sternberg, B., F.L. Sorgi and L. Huang. 1994. New structures in complex formation between DNA and cationic liposomes visualized by freeze-fracture electron microscopy. FEBS Letters 356:361-366.