Improved nucleic acid organic extraction through use of a unique gel barrier material

BioTechniques

Volumn 21, Issue 5, 1996, Pages 934-939

  Murphy, Neil R ^a   Hellwig, Randolph J ^a  

^a 5 Prime 3 Prime Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; NUCLEIC ACID; RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; EXTRACTION; GEL; NONHUMAN; NUCLEIC ACID ANALYSIS; PURIFICATION; RABBIT;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0029821308     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215pf01     Document Type: Article

References (9)

1. Birnboim, H.C. and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
2. Chirgwin, J.M., A.E. Przybyla, R.J. MacDonald and W.J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299.
3. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
4. Emanuel, J.R. 1992. Phase Lock Gel™ plasmid micropreps: direct insert screening, probe synthesis and sequencing within one day. Nucleic Acids Res. 20:625.
5. Herrmann, B.G. and A.M. Frischauf. 1987. Isolation of genomic DNA. Methods Enzymol. 152:180-183.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
8. Zervos, P.H., L.M. Morris and R.J. Hellwig. 1988. A novel method for rapid isolation of plasmid DNA. BioTechniques 6:238-242.
9. Zolo, M. and E.Y. Chen. 1994. A manual high-throughput M13 DNA preparation. BioTechniques 16:370-372.