Two consensus primer systems and nested polymerase chain reaction for human papillomavirus detection in cervical biopsies: A study of sensitivity

Human Pathology

Volumn 27, Issue 8, 1996, Pages 812-815

  Zehbe, Ingeborg ^a   Wilander, Erik ^a  

^a UPPSALA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

cervical intraepithelial neoplasia; consensus primers; human papillomavirus; polymerase chain reaction

Indexed keywords

ADULT; ARTICLE; DIAGNOSTIC APPROACH ROUTE; FEMALE; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; SINGLE STRAND CONFORMATION POLYMORPHISM; UTERINE CERVIX BIOPSY; UTERINE CERVIX CARCINOMA IN SITU; VIRUS DETECTION; WART VIRUS;

EID: 0029820996     PISSN: 00468177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0046-8177(96)90454-2     Document Type: Article

References (10)

1. Snijders PJF, van den Brule AJC, Schrijnemakers HJF et al: The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. J Gen Virol 71:173-181, 1990
2. Manos MM, Ting Y, Wright DK et al: Use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cells 7:209-214, 1989
3. De Roda Husman A-M, Walboomers JMM, van den Brule AJC et al: The use of the general primers GP5 and GP6 elongated at their 3′ ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol 76:1057-1062, 1995
4. Bauer HM, Greer CE, Manos MM: Determination of genital human papillomavirus infection by consensus PCR amplification, in Herrington CS, McGee JO'D (eds): Diagnostic Molecular Pathology, vol 2. New York, NY, Oxford University Press, 1992, pp 131-152
5. Evander M, Edlund K, Boden E et al: Comparison of a one-step and a two-step polymerase chain reaction with degenerate general primers in a population-based study of human papillomavirus infection in young Swedish women. J Clin Microbiol 30:987-992, 1992
6. Zehbe I, Rylander E, Edlund K, et al: Detection of human papillomavirus in cervical intra-epithelial neoplasia, using in situ hybridization and various polymerase chain reaction techniques, (in press)
7. Lungu O, Wright TC Jr, Silverstein S: Typing of human papillomavirus by polymerase chain reaction amplification with Ll consensus primers and RFLP analysis. Mol Cell Probes 6:145-152, 1992
8. Saiki RK, Scharf S, Faloona F et al: Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350-1354, 1985
9. Erlich HA, Gelfand D, Sninsky JJ: Recent advances in the polymerase chain reaction. Science 252:1643-1656, 1991
10. Zehbe I, Evander M, Edlund K et al: Non-radioisotopic detection of human papillomavirus by polymerase chain reaction and single strand conformation polymorphism. Am J Surg Pathol [B] Diagn Mol Pathol (in press)