In vitro inhibition of hepatitis C virus gene expression by chemically modified antisense oligodeoxynucleotides

Antimicrobial Agents and Chemotherapy

Volumn 40, Issue 10, 1996, Pages 2337-2344

  Vidalin, O ^a   Major, M E ^a,c   Rayner, B ^b   Imbach, J L ^b   Trepo C ^a   Inchauspe G ^a  

^a INSERM   (France)

^b CNRS   (France)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGODEOXYNUCLEOTIDE; CHLORAMPHENICOL ACETYLTRANSFERASE; OLIGODEOXYNUCLEOTIDE DERIVATIVE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; RIBONUCLEASE H;

ANIMAL CELL; ARTICLE; CELL FREE SYSTEM; CHEMICAL MODIFICATION; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME ASSAY; GENE EXPRESSION; HEPATITIS C VIRUS; NONHUMAN; PRIORITY JOURNAL; RETICULOCYTE LYSATE; RNA CLEAVAGE; TRANSLATION REGULATION; VIRUS INHIBITION; VIRUS TRANSCRIPTION; WHEAT GERM;

EID: 0029820484     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.40.10.2337     Document Type: Article

References (52)

1. Ali, N., and A. Siddiqui. 1995. Interaction of polypyrimidine tract-binding protein with the 5′ noncoding region of the hepatitis C virus RNA genome and its functional requirement in internal initiation of translation. J. Virol. 69:6367-6375.
2. Alt, M., R. Renz, P. H. Hofschneider, G. Paumgartner, and W. H. Caselmann. 1995. Specific inhibition of hepatitis C viral gene expression by antisense phosphorothioate oligodeoxynucleotides. Hepatology 22:707-717.
3. Alter, H. J., R. H. Purcell, J. W. Shih, J. C. Melpolder, M. Houghton, Q. L. Choo, and G. Kuo. 1989. Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. N. Engl. J. Med. 321:1494-1500.
4. Anazodo, M. I., M. A. Wainberg, A. D. Friesen, and J. A. Wright. 1995. Inhibition of human immunodeficiency virus type 1 (HIV) gag gene expression by an antisense oligodeoxynucleotide phosphorothioate. Leukemia 9:86-88.
5. Bacon, T. A., F. Morvan, B. Rayner, J.-L. Imbach, and E. Wickstrom. 1988. α-Oligonucleotide stability in serum, subcellular extracts and culture media. J. Biochem. Biophys. Methods 16:311-317.
6. Bartenschlager, R., L. Ahlborn-Laake, K. Yasargil, J. Mous, and H. Jacobsen. 1995. Substrate determinants for cleavage in cis and in trans by the hepatitis C virus NS3 proteinase. J. Virol. 69:198-205.
7. Bertrand, J.-R., B. Rayner, J.-L. Imbach, C. Paoletti, and C. Malvy. 1989. Comparative activity of α- and β-anomeric oligonucleotides on rabbit β-globin synthesis: inhibitory effects of cap targeted α-oligonucleotides. Biochem. Biophys. Res. Commun. 164:311-318.
8. Blake, K. R., A. Murakami, and P. S. Miller. 1985. Inhibition of rabbit globin mRNA translation by sequence-specific oligodeoxyribonucleotides. Biochemistry 24:6132-6138.
9. Brown, E. A., H. Zhang, L. H. Ping, and S. M. Lemon. 1992. Secondary structure of the 5′ nontranslated regions of hepatitis C virus and pestivirus genomic RNAs. Nucleic Acids Res. 20:5041-5045.
10. Bukh, J., R. H. Purcell, and R. H. Miller. 1992. Sequence analysis of the 5′ noncoding region of hepatitis C virus. Proc. Natl. Acad. Sci. USA 89:4942-4946.
11. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
12. Choo, Q. L., G. Kuo, A. J. Weiner, L. R. Overby, D. W. Bradley, and M. Houghton. 1989. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359-362.
13. Choo, Q. L., K. H. Richman, J. H. Han, K. Berger, C. Lee, C. Dong, C. Gallegos, D. Coit, R. Medina-Selby, P. J. Barr, et al. 1991. Genetic organization and diversity of the hepatitis C virus. Proc. Natl. Acad. Sci. USA 88:2451-2455.
14. Cohen, J. S. 1991. Antisense oligodeoxynucleotides as antiviral agents. Antiviral Res. 16:121-133.
15. Gagnor, C., J.-R. Bertrand, S. Thenet, M. Lemaître, F. Morvan, B. Rayner, C. Malvy, B. Lebleu, J.-L. Imbach, and C. Paoletti. 1987. α-DNA VI: comparative study of α- and β-anomeric oligodeoxynucleotides in hybridization to mRNA and in cell free translation inhibition. Nucleic Acids Res. 15: 10419-10436.
16. Ghosh, M. K., and J. S. Cohen. 1992. Oligonucleotides as antisense inhibitors of gene expression. Prog. Nucleic Acid Res. Mol. Biol. 42:79-126.
17. Grakoui, A., C. Wychowski, C. Lin, S. M. Feinstone, and C. M. Rice. 1993. Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67:1385-1395.
18. Gray, G. D., O. M. Hernandez, D. Hebel, M. Root, J. M. Pow-Sang, and E. Wickstrom. 1993. Antisense DNA inhibition of tumor growth induced by c-Ha-ras oncogene in nude mice. Cancer Res. 53:577-580.
19. Harada, S., Y. Watanabe, K. Takeuchi, T. Suzuki, T. Katayama, Y. Takebe, I. Saito, and T. Miyamura. 1991. Expression of processed core protein of hepatitis C virus in mammalian cells. J. Virol. 65:3015-3021.
20. Iacovaci, S., M. Sargiacomo, I. Parolini, A. Ponzetto, C. Peschle, and G. Carloni. 1993. Replication and multiplication of hepatitis C virus genome in human foetal liver cells. Res. Virol. 144:275-279.
21. Inchauspe, G., S. Zebedee, D. H. Lee, M. Sugitani, M. Nasoff, and A. M. Prince. 1991. Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates. Proc. Natl. Acad. Sci. USA 88:10292-10296.
22. Jang, S. K., M. V. Davies, R. J. Kaufman, and E. Wimmer. 1989. Initiation of protein synthesis by internal entry of ribosomes into the 5′ nontranslated region of encephalomyocarditis virus RNA in vivo. J. Virol. 63:1651-1660.
23. Kato, N., T. Nakazawa, T. Mizutani, and K. Shimotohno. 1995. Susceptibility of human T-lymphotropic virus type I infected cell line MT-2 to hepatitis C virus infection. Biochem. Biophys. Res. Commun. 206:863-869.
24. Lin, C., B. D. Lindenbach, B. M. Pragai, D. W. McCourt, and C. M. Rice. 1994. Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini. J. Virol. 68:5063-5073.
25. Lisziewicz, J., D. Sun, V. Metelev, P. Zarnecnik, R. C. Gallo, and S. Agrawal. 1993. Long-term treatment of human immunodeficiency virus-infected cells with antisense oligonucleotide phosphorothioates. Proc. Natl. Acad. Sci. USA 90:3860-3864.
26. Lisziewicz, J., D. Sun, F. F. Weichold, A. R. Thierry, P. Lusso, J. Tang, R. C. Gallo, and S. Agrawal. 1994. Antisense oligodeoxynucleotide phosphorothioate complementary to Gag mRNA blocks replication of human immunodeficiency virus type 1 in human peripheral blood cells. Proc. Natl. Acad. Sci. USA 91:7942-7946.
27. Milligan, J. F., M. D. Matteucci, and J. C. Martin. 1993. Current concepts in antisense drug design. J. Med. Chem. 36:1923-1937.
28. Mizutani, T., N. Kato, M. Hirota, K. Sugiyama, A. Murakami, and K. Shimotohno. 1995. Inhibition of hepatitis C virus replication by antisense oligonucleotide in culture cells. Biochem. Biophys. Res. Commun. 212:906-911.
29. Morvan, F., B. Rayner, and J.-L. Imbach. 1990. Alpha-oligonucleolides (alpha-DNA): a new chimeric nucleic acid analog. p. 37-52. In E. J. K. Setlows (ed.), Genetic engineering: principles and methods, vol. 12. Plenum Press, New York.
30. Morvan, F., B. Rayner, J.-L. Imbach, S. Thenet, J.-R. Bertrand, J. Paoletti, C. Malvy, and C. Paoletti. 1987. α-DNA. II. Synthesis of unnatural α-anomeric oligodeoxynucleotides containing the four usual bases and study of their substrate activities for nucleases. Nucleic Acids Res. 15:3421-3437.
31. Offensperger, W. B., S. Offensperger, E. Walter, K. Teubner, G. Igloi, H. E. Blum, and W. Gerok. 1993. In vivo inhibition of duck hepatitis B virus replication and gene expression by phosphorothioate modified antisense oligodeoxynucleotides. EMBO J. 12:1257-1262.
32. Pelham, H. U. B., and R. J. Jackson. 1976. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem. 67:247-256.
33. Pelletier, J., and N. Sonenberg. 1988. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature (London) 334:320-325.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Shimizu, Y. K., A. Iwamoto, M. Hijikata, R. H. Purcell, and H. Yoshikura. 1992. Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proc. Natl. Acad. Sci. USA 89:5477-5481.
36. Stein, C. A., and Y. C. Cheng. 1993. Antisense oligonucleotides as therapeutic agents - is the bullet really magical? Science 261:1004-1012.
37. Stein, C. A., J. L. Tonkinson, and L. Yakubov. 1991. Phosphorothioate oligodeoxynucleotides - anti-sense inhibitors of gene expression? Pharmacol. Ther. 52:365-384.
38. Szczylik, C., T. Skorski, N. C. Nicolaides, L. Manzella, L. Malaguarnera, D. Venturelli, A. M. Gewirtz, and B. Calabretta. 1991. Selective inhibition of leukemia cell proliferation by BCR-ABL antisense oligodeoxynucleotides. Science 253:562-565.
39. Tine, F., S. Magrin, A. Craxi, and L. Pagliaro. 1991. Interferon for non-A, non-B chronic hepatitis. A meta-analysis of randomized clinical trials. J. Hepatol. 13:192-199.
40. Tsukiyama-Kohara. K., N. Iizuka, M. Kohara, and A. Nomoto. 1992. Internal ribosome entry site within hepatitis C virus RNA. J. Virol. 66:1476-1483.
41. Tsukuma, H., T. Hiyama, S. Tanaka, N. Nakao, T. Yabuuchi, T. Kitamura, K. Nakanishi, I. Fujimoto, A. Inoue, H. Yamazaki, and T. Kawashima. 1993. Risk factors for hepatocellular carcinoma among patients with chronic liver disease. N. Engl. J. Med. 328:1797-1801.
42. Tymms, M. J. 1995. In vitro transcription and translation protocols. Methods Mol. Biol. 37:215-232, 245-263.
43. Uhlmann, E., and A. Peyman. 1990. Antisense oligonucleotides: a new therapeutic principle. Chem. Rev. 90:544-584.
44. Wagner, R. W., M. D. Matteucci, J. G. Lewis, A. J. Gutierrez, C. Moulds, and B. C. Froehler. 1993. Antisense gene inhibition by oligonucleotides containing C-5 propyne pyrimidines. Science 250:1510-1513.
45. Wakita, T., and J. R. Wands. 1994. Specific inhibition of hepatitis C virus expression by antisense oligodeoxynucleotides. In vitro model for selection of target sequence. J. Biol. Chem. 269:14205-14210.
46. Wang, C., and S.-Y. Le. 1995. An RNA pseudoknot is essential for internal initiation of translation of hepatitis C virus RNA genome. RNA 1:526-537.
47. Wang, C., P. Sarnow, and A. Siddiqui. 1993. Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism. J. Virol. 67:3338-3344.
48. Wang, C., P. Sarnow, and A. Siddiqui. 1994. A conserved helical element is essential for internal initiation of translation of hepatitis C virus RNA. J. Virol. 68:7301-7307.
49. Wang, C., and A. Siddiqui. 1995. Structure and function of the hepatitis C virus internal ribosome entry site. Curr. Top. Microbiol. 203:99-115.
50. Whetter, L. E., S. P. Day, O. Elroy-Stein, E. A. Brown, and S. M. Lemon. 1994. Low efficiency of the 5′ nontranslatcd region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells. J. Virol. 68:5253-5263.
51. Yoo, B. J., M. J. Selby, J. Choe, B. S. Suh, S. H. Choi, J. S. Joh, G. J. Nuovo, H. S. Lee, M. Houghton, and J. H. Han. 1995. Transfection of a differentiated human hepatoma cell line (Huh7) with in vitro-transcribed hepatitis C virus (HCV) RNA and establishment of a long-term culture persistently infected with HCV. J. Virol. 69:32-38.
52. Zelphati, O., J. L. Imbach, N. Signoret, G. Zon, B. Rayner, and L. Leserman. 1994. Antisense oligonucleotides in solution or encapsulated in immunoliposomes inhibit replication of HIV-1 by several different mechanisms. Nucleic Acids Res. 22:4307-4314.