Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii

Journal of Bacteriology

Volumn 178, Issue 19, 1996, Pages 5793-5796

  Seyfried, Markus ^a   Daniel, Rolf ^a   Gottschalk, Gerhard ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COBAMAMIDE; HYDROLYASE;

AMINO ACID SEQUENCE; ARTICLE; CITROBACTER FREUNDII; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

EID: 0029818529     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.19.5793-5796.1996     Document Type: Article

References (29)

1. Andersson, L. O., H. Borg, and M. Mikaelsson. 1972. Molecular weight estimation of proteins by electrophoresis in polyacrylamide gel of graded porosity. FEBS Lett. 20:199-202.
2. Blum, H., H. Beier, and H. J. Gross. 1987. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93-99.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Daniel, R. 1994. Glycerinvergärung durch Citrobacter freundii: Sequenzierung und Charakterisierung des dha Regulons. Cuvillier Verlag, Göttingen, Germany.
5. Daniel, R., R. Boenigk, and G. Gottschalk. 1995. Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli. J. Bacte- riol. 177:2151-2156.
6. Daniel, R., and G. Gottschalk. 1992. Growth temperature-dependent activity of glycerol dehydratase in Escherichia coli expressing the Citrobacter freundii dha regulon. FEMS Microbiol. Lett. 100:281-286.
7. Daniel, R., K. Stuertz, and G. Gottschalk. 1995. Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii. J. Bacteriol. 177:4392-4401.
8. 12-binding domains of methionine synthase. Science 266:1669-1674.
9. Forage, R. G., and M. A. Foster. 1979. Resolution of the coenzyme B-12-dependent dehydratases of Klebsiella sp. and Citrobacter freundii. Biochim. Biophys. Acta 569:249-258.
10. Forage, R. G., and E. C. C. Lin. 1982. dha system mediating aerobic and anaerobic dissimilation of glycerol in Klebsiella pneumoniae NCIB 418. J. Bacteriol. 151:591-599.
11. Grower, M. F., and E. D. Bransome, Jr. 1970. Liquid scintillation counting of macromolecules in acrylamide gel, p. 263-269. In D. J. Bransome, Jr. (ed.), The current status of liquid scintillation counting. Grune and Stratton, New York.
12. Johnson, E. A., S. K. Burke, R. G. Forage, and E. C. C. Lin. 1984. Purification and properties of dihydroxyacetone kinase from Klebsiella pneumoniae. J. Bacteriol. 160:55-60.
13. Marsh, E. N. G., and D. E. Holloway. 1992. Cloning and sequencing of glutamate mutase component S from Clostridium tetanomorphum. Homologies with other cobalamin-dependent enzymes. FEBS Lett. 310:167-170.
14. Pawelkiewicz, J., and B. Zagalak. 1965. Enzymic conversion of glycerol into β-hydroxypropionaldehyde in a cell-free extract from Aerobacter aerogenes. Acta Biochim. Pol. 12:207-218.
15. 12-dependent diol dehydrase: purification, subunit heterogeneity, and reversible association. Arch. Biochem. Biophys. 194:379-386.
16. Ruch, F. E., J. Lengeler, and E. C. C. Lin. 1974. Regulation of glycerol catabolism in Klebsiella aerogenes. J. Bacteriol. 119:50-56.
17. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
18. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
19. Schägger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
20. Schneider, Z., E. G. Larsen, G. Jacobson, B. C. Johnson, and J. Pawelklewicz. 1970. Purification and properties of glycerol dehydrase. J. Biol. Chem. 245:3388-3396.
21. Schneider, Z., and J. Pawelkiewicz. 1966. The properties of glycerol dehydratase isolated from Aerobacter aerogenes, and the properties of the apoenzyme subunits. Acta Biochim. Pol. 13:311-327.
22. Siegel, L. M., and K. J. Monty. 1966. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim. Biophys. Acta 112:346-362.
23. Smiley, K. L., and M. Sobolov. 1962. A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus. Arch. Biochem. Biophys. 97:538-543.
24. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
25. Talarico, T. L., and W. J. Dobrogosz. 1990. Purification and characterization of glycerol dehydratase from Lactobacillus reuteri. Appl. Environ. Microbiol. 56:1195-1197.
26. Tobimatsu, T., T. Hara, M. Sakaguchi, V. Kishimoto, Y. Wada, M. Isoda, T. Sakal, and T. Toraya. 1995. Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca. J. Biol. Chem. 270:7142-7148.
27. 12-dependent diol dehydratase and glycerol dehydratase. Eur. J. Biochem. 76:285-289.
28. 12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae. J. Bacteriol. 141:1439-1442.
29. Weber, K., and M. Osborn. 1969. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406-4412.