Analysis of exon and intron mutants in the cytochrome b mitochondrial gene of Saccharomyces cerevisiae

Current Genetics

Volumn 30, Issue 3, 1996, Pages 200-205

  Bonjardim, Claudio A ^a   Pereira, Lourivaldo S ^b   Nobrega, F G ^b  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Cytochrome b; Mitochondria; Mutants; Saccharomyces cerevisiae

Indexed keywords

CELL PROTEIN; CYTOCHROME B; MITOCHONDRIAL RNA; PROTEIN SUBUNIT;

AMINO ACID SUBSTITUTION; ARTICLE; EXON; GENE MAPPING; GENE MUTATION; GENE STRUCTURE; IMMUNOBLOTTING; INTRON; MISSENSE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RNA PROCESSING; SACCHAROMYCES CEREVISIAE; STOP CODON;

APOPROTEINS; CODON; CYTOCHROME B GROUP; CYTOCHROMES B; DNA, MITOCHONDRIAL; ELECTRON TRANSPORT COMPLEX III; EXONS; INTRONS; MITOCHONDRIA; MUTATION; POINT MUTATION; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 0029818456     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002940050121     Document Type: Article

References (40)

1. Berden JA, Schoppink PJ, Grivell LA (1988) A model for the assembly of the ubiquinol-cytochrome c oxidoreductase in yeast. In: Palmieri F, Quagliarello E (eds) Molecular basis of biomembrane transport. Elsevier Science Publishers, Amsterdam, pp 195-208
2. Bonitz SG, Homison G, Thalenfeld BE, Tzagoloff A, Nobrega FG (1982) Assembly of the mitochondrial membrane system. Processing of the apocytochrome b precursor RNAs in Saccharomyces cerevisiae D273-10B. J Biol Chem 257:6268-6274
3. Bonjardim CA, Nobrega FG (1984 a) Revision of the nucleotide sequence at the last intron of the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae. Braz J Med Biol Res 17:17-20
4. Bonjardim CA, Nobrega FG (1984 b) Nucleotide substitutions in a yeast mitochondrial cis-acting mutant located in the last intron of the apocytochrome b gene. FEBS Lett 169:73-78
5. Burke JM, Belfort M, Cech TR, Wayne Davies R, Schweyen RJ, Shub DA, Szostak JW, Tabak HF (1987) Structural conventions for group-I introns. Nucleic Acids Res 15:7217-7221
6. Burke JM, Esherick JS, Burfeind WR, King JL (1990) A 3′ splice site-binding sequence in the catalytic core of a group-I intron. Nature 344:80-82
7. Church GM, Slonimski PP, Gilbert W(1979) Pleiotropic mutations within two yeast mitochondrial genes block mRNA processing. Cell 18:1209-1215
8. Coruzzi G, Kaye Trembath M, Tzagoloff A (1979) Isolation of mitochondrial and nuclear mutants of Saccharomyces cerevisiae with specific defects in mitochondrial function. Methods Enzymol 56:95-106
9. Crivellone MD, Wu M, Tzagoloff A (1988) Assembly of the mitochondrial membrane system. Analysis of structural mutants of the yeast coenzyme QH2-cytochrome c reductase complex. J Biol Chem 263:14323-14333
10. De Haan M, Van Loon APGM, Kreike J, Vaessen RTMJ, Grivell LA (1984) The biosynthesis of the ubiquinol-cytochrome c reductase complex in yeast. DNA sequence analysis of the nuclear gene coding for the 14-kDa subunit. Eur J Biochem 138:169-177
11. Delouya D, Nobrega FG (1991) Mapping of the ARS-like activity and transcription initiation sites in the non-canonical yeast mitochondrial ori6 region. Yeast 7:51-60
12. Dieckmann CL, Tzagoloff A (1983) Analysis of yeast mitochondrial genes. Methods Enzymol 97:361-373
13. Gampel. A, Cech TR (1991) Binding of the CBP2 protein to a yeast mitochondrial group-I intron requires the catalytic core of the RNA. Genes Dev 5:1870-1880
14. Gampel A, Tzagoloff A (1987) In vitro splicing of the terminal intervening sequence of Saccharomyces cerevisiae cytochrome b pre- mRNA. Mol Cell Biol 7:2545-2551
15. Goguel V, Perea J, Jacq C (1989) Synthesis and function of the mitochondrial intron-encoded bI4 RNA maturase from Saccharomyces cerevisiae. Curr Genet 16:241-246
16. Haycock JW (1993) Polyvinylpyrrolidone as a blocking agent in immunochemical studies. Anal Biochem 208:397-399
17. - mutations in the mitochondrial oli2 gene coding for mitochondrial ATPase subunit 6 in Saccharomyces cerevisiae. Nucleic Acids Res 14:7437-7451
18. Katan MB, Harten-Loosbroek NV, Groot GSP (1976) The cytochrome bc 1 complex of yeast mitochondria. Site of translation of the polypeptides in vivo. Eur J Biochem 70:409-417
19. Kübrich M, Dietmer K, Pfanner N (1995) Genetic and biochemical dissection of the mitochondrial protein-import machinery. Curr Genet 27:393-403
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
21. Lazowska J, Jacq C, Slonimski PP(1980) Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron. Cell 22:333-348
22. Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:449-560
23. McGraw P, Tzagoloff A (1983) Assembly of the mitochondrial membrane system. Characterization of a yeast nuclear gene involved in the processing of the cytochrome b pre-mRNA. J Biol Chem 258:9459-9468
24. Michel F, Hanna M, Green R, Bartel DP, Szostak J (1989) The guanosine-binding site of the Tetrahymena ribozyme. Nature 342: 391-395
25. Nobrega FG, Tzagoloff A (1980 a) Assembly of the mitochondrial membrane system.Complete restriction map of the cytochrome b region of mitochondrial DNA in Saccharomyces cerevisiae D273-10B. J Biol Chem 255:9821-9827
26. Nobrega FG, Tzagoloff A (1980 b) Assembly of the mitochondrial membrane system. DNA sequence and organization of the cytochrome b gene in Saccharomyces cerevisiae D273-10B. J Biol Chem 255:9828-9837
27. Nobrega FG, Dieckmann CL, Tzagoloff A (1983) A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution. Anal Biochem 131:141-145
28. Phillips JD, Graham LA, Trumpower BL (1993) Subunit 9 of the Saccharomyces cerevisiae cytochrome bc1 complex is required for insertion of the EPR-detectable iron-sulfur cluster into the Rieske iron-sulfur protein. J Biol Chem 268:11727-11736
29. Putrament A, Baranowska H, Prazmo W (1973) Induction by manganese of mitochondrial antibiotic resistance mutations in yeast. Mol Gen Genet 126:357-366
30. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
31. Schuler GD, Altschul SF, Lipman DJ (1991) A workbench for multiple alignment construction and analysis. Proteins: Struct, Function Genet 9:180-190
32. 1. Arch Biochem Biophys 242:393-401
33. Slonimski PP, Tzagoloff A (1976) Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and/or coenzyme QH2-cytochrome c reductase. Eur J Biochem 61:27-41
34. Stephenson G, Marzuki S, Linnane AW (1980) Two-dimensional electrophoretic analysis of mitochondrial translation products in yeast. Biochim Biophys Acta 609:329-341
35. Trumpower BL, Gennis RB (1994) Energy transduction by cytochrome complexes in mitochondrial and bacterial respiration: the enzymology of coupling electron transfer reactions to transmembrane proton translocation. Annu Rev Biochem 63:675-716
36. Tzagoloff A, Akai A, Needleman RB (1975 a) Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions. J Bacteriol 122:826-831
37. Tzagoloff A, Akai A, Needleman RB, Zulch G (1975 b) Assembly of the mitochondrial membrane system. Cytoplasmic mutants of Saccharomyces cerevisiae with lesions in enzymes of the respiratory chain and in the mitochondrial ATPase. J Biol Chem 250: 8236-8242
38. Tzagoloff A, Foury F, Akai A (1976) Assembly of the mitochondrial membrane system. XVIII. Genetic loci on mitochondrial DNA involved in cytochrome b biosynthesis. Mol Gen Genet 149: 33-42
39. Weeks KM, Cech TR (1995) Efficient protein-facilitated splicing of the yeast mitochondrial bI5 intron. Biochemistry 34:7728-7738
40. Weiss-Brummer B, Hüttenhofer A, Kaudewitz F (1984) Leakiness of termination codons in mitochondrial mutants of the yeast Saccharomyces cerevisiae. Mol Gen Genet 198:62-68