Temperature-programmed capillary electrophoresis for detection of DNA point mutations

BioTechniques

Volumn 21, Issue 5, 1996, Pages 926-932

  Gelfi, Cecilia ^a   Cremonesi, Laura ^b   Ferrari, Maurizio ^b   Righetti, Pier Giorgio ^c,d  

^a CNR   (Italy)

^b SAN RAFFAELE HOSPITAL   (Italy)

^c UNIVERSITY OF CALABRIA   (Italy)

^d LITA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA FRAGMENT; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; HUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; TEMPERATURE; ZONE ELECTROPHORESIS;

EID: 0029818082     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215rr06     Document Type: Article

References (22)

1. Belle, M.S., M. Chiari, M. Nesi and P.G. Righetti. 1992. Prediction of current-voltage dependence and electrochemical calibration for capillary zone electrophoresis. J. Chromatogr. 652:323-330.
2. Bello, M.S., E.I. Levin and P.G. Righetti. 1993. Computer-assisted determination of the inner temperature and peak correction for capillary electrophoresis. J. Chromatogr. 652: 329-336.
3. Brancolini, V., L. Cremonesi, E. Belloni, E. Pappalardo, R Bordoni, M. Seia, S. Russo, R. Padoan, A. Giunta and M. Ferrari. 1995. Search for mutations in pancreatic sufficient cystic fibrosis Italian patients: detection of 90% of molecular defects and identification of three novel mutations. Hum. Genet. 96:312-318.
4. Chang, H.T. and E.S. Yeung. 1993. Voltage programming in capillary zone electrophoresis. J. Chromatogr. 632:149-156.
5. Cheadle, J.P., E. Belloni, M. Ferrari, L. Millar-Jones and A.L. Meredith. 1994. A novel mutation (M1V) in the translation initiation codon of the cystic fibrosis transmembrane conductance regulator gene, in three CF chromosomes of Italian origin. Hum. Mol. Genet. 3:1431-1432.
6. Chiari, M., M. Nesi and P.G. Righetti. 1994. Capillary zone electrophoresis of DNA fragments in a novel polymer network: poly(N-acryloyl amino ethoxy ethanol). Electrophoresis 15:616-622.
7. Devoto, M., P. Ronchetto, P. Fanen. J.J. Telleria Orriols, G. Romeo, M. Goossens, M. Ferrari, C. Magnani, M. Seia and L. Cremonesi. 1991. Screening for non-deltaF508 mutations in five exons of the cystic fibrosis transmembrane conductance regulator gene in Italy. Am. J. Hum. Genet. 48:1127-1132.
8. Fisher, S.G. and L.S. Lerman. 1982. Analysis of point mutations by denaturing gradient gel electrophoresis. Proc. Natl. Acad. Sci. USA 80:1579-1583.
9. Guillermit, H., P. Fanen and C. Ferec. 1990. A 3′ splice site consensus sequence mutation in the cystic fibrosis gene. Hum. Genet. 85:450-453.
10. Harrison, D.J., K. Fluri, K. Seiler, Z. Fan, C.S. Effenhauser and A. Manz. 1993. Micromachining a miniaturized capillary electrophoresis-based chemical analysis system on a chip. Science 267:895-897.
11. Huang, X.C., M.A. Quesada and R.A. Mathies. 1992. DNA sequencing using capillary electrophoresis. Anal. Chem. 64:2149-2154.
12. Jorgenson, J.N. and K.D. Lucas. 1983. Capillary zone electrophoresis. Science 222:266-272.
13. Kälin, A., T. Dörk, D. Bozon and B. Tümmler. 1992. A novel frame-shift mutation in exon 4 of the cystic fibrosis gene (435insA) demonstrates the ambiguity of restriction analysis for mutation screening. Hum. Mol. Genet. 1:545-546.
14. Kerem, B., J. Zielenski, D. Markiewicz, D. Bozon, E. Gazit, J. Jahaf, D. Kennedy, J. Riordan, F. Collins, J. Rommens and L.-C. Tsui. 1990. Identification of mutations in regions corresponding to the two putative nucleotide (ATP)-binding folds of the cystic fibrosis gene. Proc. Natl. Acad. Sci. USA 57:8447-8451.
15. Khrapko, K., J.S. Hanekamp, W.G. Thilly, A. Belenkii, F. Foret and B.L. Karger. 1994. Constant denaturant capillary electrophoresis:a high resolution approach to mutational analysis. Nucleic Acids Res. 22:364-370.
16. Lerman, L. and K. Silverstein. 1987. Searching for gene defects by denaturing gradient gel electrophoresis. Methods Enzymol. 155:482-495.
17. Mitnik, L., C. Heller, J. Prost and J.L. Viovy. 1995. Segregation of DNA solutions induced by electric fields. Science 267:219-222.
18. Riesner, D., K. Henco and G. Steger. 1991. Temperature gradient gel electrophoresis:a method for the analysis of conformational transitions and mutations in nucleic acids and proteins. Adv. Electrophoresis 4:169-250.
19. Righetti, P.G. and C. Gelfi. 1996. Capillary electrophoresis of DNA, p. 431-476. In P.G. Righetti (Ed.), Capillary Electrophoresis in Analytical Biotechnology. CRC Press, Boca Raton.
20. Ueno, K. and E.S. Yeung. 1994. Simultaneous monitoring of DNA fragments separated by capillary electrophoresis in a multiplexed array of 100 capillaries. Anal. Chem. 66:1424-1431.
21. Zielenski, J., D. Bozon, B.S. Kerem, D. Markievicz, P. Durie, J. Rommens and L.-C. Tsui. 1991. Identification of mutations in exons 1 through 8 of the cystic fibrosis transmembrane conductance regulator gene. Genomics 10:229-235.
22. Zielenski, J., R. Rozmahel, D. Bozon, B. Kerem, Z. Grzelczak, J. Riordan, J. Rommens and L.-C. Tsui. 1991. Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator gene. Genomics 10: 214-228.