Fate of genetically modified Rhizobium leguminosarum biovar viciae during long-term storage of commercial inoculants

Journal of Applied Bacteriology

Volumn 81, Issue 3, 1996, Pages 319-328

  Corich, V ^a   Bosco, F ^a   Giacomini, A ^a   Basaglia, M ^b   Squartini, A ^b   Nuti, M P ^b  

^a Dipto di Biotecnologie Agrarie   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BATCH CELL CULTURE; DNA; ESCHERICHIA COLI; GROWTH KINETICS;

GENE CASSETTES; GENETIC STABILITY; GENETICALLY MODIFIED; INOCULA; LONG-TERM STORAGE; MERCURY RESISTANCE; MICRO-ORGANISMS; REGULATORY STRUCTURE; RHIZOBIUM LEGUMINOSARUM; STRAIN GROWTHS;

GENES;

BIOLOGICAL MARKER;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC ENGINEERING; INOCULATION; NONHUMAN; PLASMID; RHIZOBIUM LEGUMINOSARUM; STORAGE;

EID: 0029817525     PISSN: 00218847     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.1996.tb04334.x     Document Type: Article

References (41)

1. Alberghina, L., Frontali, L. and Sensi, P. (1994) Proceedings of the 6th European Congress on Biotechnology. Amsterdam: Elsevier Science Publishers.
2. Ausubel, P.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1987) Current Protocols in Molecular Biology. New York, NY: Wiley Publishers.
3. Awong, J., Bitton, G. and Chaudry, G.R. (1990) Microcosm for assessing survival of genetically engineered microorganisms in aquatic environments. Applied and Environmental Microbiology 56, 977-983.
4. Beringer, J.G. (1974) R-factor transfer in Rhizobium leguminosarum. Journal of General Microbiology 84, 188-198.
5. Birkenhead, K., Wang, Y.P., Noonan, B., Manian, S.S. and O'Gara, F. (1990) Characterization of Rhizobium meliloti del genes and relationships between utilization of dicarboxylic acid and expression of nitrogen fixation genes. In Advances in Molecular Genetics of Plant-Microbe Interactions ed. Palacios, R. and Verma, D.P. p. 834. American Phytopathology Society. St Paul Publishers.
6. Bishop, D.H.L., Entwistle, P.P., Cameroon, I.R., Allen, CJ. and Possee, R.D. (1988) Field trials of genetically engineered Baculovirus insecticides. In Release of Genetically Engineered Microorganisms ed. Sussman, M. et al. pp. 143-179. Academic Press.
7. Bosworth, A.H., Williams, M.K., Albrecht, K.A., Kwiatkowski, R., Beynon, J., Hankinson, T.R., Ronson, C.W., Cannon, P., Wacek, T.J. and Triplet!, E.W. (1994) Alfalfa yeld response to inoculation with recombinant strains of Rhizobium meliloti with an extra copy of dctABD and/or modified nifA expression. Applied and Environmental Microbiology 60, 3815-3832.
8. Caulcott, C.A. (1984) Competition between plasmid-positive and plasmid-negative cells. Biochemical Society Transactions 12, 1140-1142.
9. Caulcott, C.A., Lilley, G., Wright, E.M., Robinson, M.K. and Yarranton, G.T. (1985) Investigation on the instability of plasmids directing the expression of Met-prochymosin in Escherichia coli. Journal of General Microbiology 131, 3355-3365.
10. Fahraeus, G. (1957) The infection of clover root hairs by nodule bacteria studied by a simple glass technique. Journal of General Microbiology 16,374-381.
11. Géniaux, E. and Amarger, N. (1993) Diversity and stability of plasmid transfer in isolates from a single field population of Rhizobium leguminosarum bv. viciae. FEAiS Microbiology Ecology 102,251-260.
12. Giacomini, A., Corich, V., Ollero, F.J., Squartini, A. and Nuti, M.P. (1992) Experimental conditions may affect the reproducibility of the /i-galactosidase assay. FEMS Microbiology Letters 100, 87-90.
13. Giacomini, A., Ollero, F. J., Squartini, A. and Nuti, M. P. (1994) Construction of multipurpose gene cartridges based on a novel synthetic promoter for high-level gene expression in Gram-negative bacteria. Gene 144, 17-24.
14. Glenn, A.R. and Dilworth, MJ. (1981) The uptake and hydrolysis of disaccharides by fast- and slow-growing species of Rhizobium. Archives of Microbiology 129, 233-239.
15. Graham-Weiss, L., Bennett, M.L. and Paau, A.S. (1987) Production of bacterial inoculants by direct fermentation on nutrient-supplemented vermiculite. Applied and Environmental Microbiology 53, 2138-2140.
16. Iwasaki, K., Uchiyama, H. and Yagi, O. (1993) Survival and impact of genetically engineered Pseudomonas putida harboring mercury resistance gene in aquatic microcosms. Bioscience, Biotechnology and Biochemistry 57, 1264-1269.
17. Jones, D.A., Ryder, M.H., Clare, B.C., Farrand, S.K. and Kerr, A. (1988) Construction of a tra deletion mutant of pAgK84 to safeguard the biological control of crown gall. Molecular and General Genetics 212, 207.
18. Jones, D.D (ed.) (1994) Proceedings of the 3rd International Symposium on the Biosafety Results of Field Tests of Genetically Modified Plants and Microorganisms, Monterey, CA.
19. Kinkle, B.K., Sadowsky, M.J., Schmidt, E.L. and Koskinen, W.C. (1993) Plasmids pJP4 and r68.45 can be transferred between populations of bradyrhizobia in nonsterile soil. Applied and Environmental Microbiology 59, 1762-1766.
20. Lindow, S.E. (1991) Tests of specificity of competition around Pseudomonas syringae strains on plants using recombinant icenucleation genes as probes of in situ transcriptional activity. In Advances in Molecular Genetics of Plant-Microbe Interactions ed. Hennecke, H. and Verma, D.P.S. pp. 457-464. Dordrecht: Kluwer Academic Publishers.
21. Lupi, F., Casella, S., Toffanin, A. and Squartini, A. (1988) Introduction of Rhizobium 'hedysarf in alkaline clay-loam soil by different inoculation techniques. Arid Soil Research and Rehabilitation 2, 19-28.
22. Materon, L.A. and Weaver, R.W. (1985) Inoculant maturity influences survival of rhizobia on seed. Applied and Environmental Microbiology 49, 46567.
23. McClure, N.C., Weightman, A.J. and Fry, J.C. (1989) Survival of Pseudomonas putida UWC1 containing cloned catabolic genes in a model activated-sludge unit. Applied and Environmental Microbiology 55, 2627-2634.
24. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
25. Nuti, M.P., Squartini, A. and Giacomini, A. (1994a) European community regulation for the use and release of genetically modified organisms (GMOs) in the environment. In Molecular Ecology of Rhizosphere Microorganisms ed. O'Gara, F., Dowling, D.N. and Boesten, B. pp. 165-173. New York: VCH Publishers Inc.
26. Nuti, M.P., Squartini, A., Giacomini, A., Casella, S. and Corich, V. (1994b) The use of genetically modified organisms (GMOs) in the environment: biosafety results of field tests and key scientific issues. In Proceedings of the 6th European Congress on Biotechnology ed. Alberghina, L., Frontali, L. and Sensi P. Vol. II, pp. 12911296. Amsterdam: Elsevier Science Publishers.
27. Orvos, D.R., Lacy, G.H. and Cairns, J. (1990) Genetically engi-neered Errvinia carotovora: survival, intraspecific competition, and effects upon selected bacterial genera. Applied and Environmental Microbiology 56, 1689-1694.
28. Richaume, A., Smit, E., Faurie, G. and van Elsas, J.D. (1992) Influence of soil type on the transfer of plasmid RP4p from Pseudomonas fluorescens to introduced recipient and to indigenous bacteria. FEMS Microbiology Ecology 101, 281-292.
29. Robinson, J.B. and Tuovinen, O.H. (1984) Mechanism of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical and genetic analyses. Microbiological Reviews 48, 95-124.
30. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
31. Scanferlato, V.S., Orvos, D.R., Cairns, J. and Lacy, G.H. (1990) Genetically engineered Erwinia carotovora in aquatic microcosms: survival and effects on functional groups of indigenous bacteria. Applied and Environmental Microbiology 55, 1477-1482.
32. Schmidhauser, T.J., Ditta, G. and Helinski, D.R. (1988) Broadhost-range plasmid cloning vectors for gram-negative bacteria. In Vectors: A Survey of Molecular Cloning Vectors and Their Uses ed. Rodriguez, R.L. and Denhardt, D.T. pp. 287-332. Stoneham, MA: Butterworth Publishers.
33. Selbitschka, W., Arnold, W., Priefer, U.B., Rottschäfer, T., Schmidt, M., Simon, R. and Fühler, A. (1991) Characterization of recA genes and recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae. Molecular and General Genetics 229, 86-95.
34. Silver, S. and Misra, T.K. (1988) Plasmid-mediated heavy metal resistances. Annual Review of Microbiology 42, 717-743.
35. Simon, R., Priefer, U. and Fühler, A. (1983) A broad host range mobilization system for in vitro genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1, 748-751.
36. Somasegaran, P. (1985) Inoculant production with diluted liquid cultures of Rhizobium spp. and autoclaved peat: evaluation of diluents, Rhizobium spp., peats, sterility requirements, storage, and plant effectiveness. Applied and Environmental Microbiology 50, 398-405.
37. Tas, E., Saano, A., Leinonen, P. and Lindström, K. (1995) Identification of Rhizobium spp. in peat-based inoculants by DNA hybridization and PCR and its application in inoculant quality control. Applied and Environmental Microbiology 61, 1822-1827.
38. Vincent, J.M. (1910) A Manual for the Practical Studies of the Nodule. IBP Handbook no. 15. Oxford: Blackwell Science.
39. Wilson, M. and Lindow, S.E. (1993) Release of recombinant microorganisms. Annual Review of Microbiology 47, 913-944.
40. Zund, P. and Lebek, G. (1980) Generation time-prolonging R plasmids: correlation between increases in generation time of Escherichia coli caused by R plasmid and their molecular size. Plasmid 3, 65-69.
41. Zwietering, M.H., Jongenburger, I., Rombout, F.M. and van't Riet, K. (1990) Modeling of the bacterial growth curve. Applied and Environmental Microbiology 56, 1875-1881.