Optimization of a mammalian expression system for the measurement of sodium channel gating currents

American Journal of Physiology - Cell Physiology

Volumn 271, Issue 3 40-3, 1996, Pages

  Sheets, Michael F ^a   Kyle, John W ^b   Krueger, Stephanie ^b   Hanck, Dorothy A ^b  

^a NORTHWESTERN UNIVERSITY   (United States)

^b NONE

Author keywords

fusion; polyethylene glycol; sodium current

Indexed keywords

COMPLEMENTARY DNA; MACROGOL; SODIUM CHANNEL; SODIUM ION;

ARTICLE; CELL FUSION; CHANNEL GATING; CONTROLLED STUDY; DNA TRANSFECTION; HUMAN; HUMAN CELL; MAMMAL CELL; MULTINUCLEAR CELL; PRIORITY JOURNAL; SODIUM CURRENT; VOLTAGE CLAMP;

EID: 0029817157     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1996.271.3.c1001     Document Type: Article

References (22)

1. Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts, and J. D. Watson. Molecular Biology of the Cell (3rd ed.). New York: Garland, 1994.
2. Bangalore, R., G. Mehrke, K. Gingrich, F. Hofmann, and R. S. Kass. Gating currents of recombinant cardiac L-type calcium channels expressed in human embryonic kidney (HEK-293) cells: relationship between charge movement and ionic current (Abstract). Biophys. J. 68: A14, 1995.
3. Bouachour, G., G. Planelles, and T. Anagnostopoulos. Fusion of amphibian proximal convoluted cells into giant cells. Pfluegers Arch. 441: 220-222, 1988.
4. Chahine, M., A. L. George, Jr., M. Zhou, S. Ji, W. Sun, R. L. Barchi, and R. Horn. Sodium channel mutations in paramyotonia congenita uncouple inactivation from activation. Neuron 12: 281-294, 1994.
5. Conti, F., and W. Stühmer. Quantal charge redistribution accompanying the structural transition of sodium channels. Eur. Biophys. J. 17: 53-59, 1989.
6. Hanck, D. A., and M. F. Sheets. Time-dependent changes in the kinetics of the sodium current in single canine cardiac Purkinje cells. Am. J. Physiol. 262 (Heart Circ. Physiol. 31): H1197-H1207, 1992.
7. Hanck, D. A., M. F. Sheets, and H. A. Fozzard. Gating currents associated with Na channels in canine cardiac Purkinje cells. J. Gen. Physiol. 95: 439-457, 1990.
8. + channel inactivation gating. Circ. Res. 75: 114-122, 1994.
9. + channels. J. Gen. Physiol. 106: 1053-1068, 1995.
10. Kersting, U., H. Joha, W. Steigner, B. Gassner, G. Gstraunthaler, W. Pfaller, and H. Oberleithner. Fusion of cultured dog kidney (MDCK) cells. I. Technique, fate of plasma membranes and of cell nuclei. J. Membr. Biol. 111: 37-48, 1989.
11. Kontis, K. J., F. Bezanilla, and A. L. Goldin. Gating current studies of the the rat brain 2A sodium channel expressed in Xenopus oocytes. Neurosci. Abstr. 20: 66, 1994.
12. Leous, M. R. An overview of the polyethylene glycols. In: Polyethylene Glycol as an Embedment for Microscopy and Histochemisiry, edited by K. Gao. Boca Raton, FL: CRC, 1993, p. 1-20.
13. Margolskee, R. F., B. McHendry-Rinde, and R. Horn. Panning transfected cells for electrophysiological studies. Biotechniques 15: 906-911, 1993.
14. Neely, A., X. Wei, R. Olcese, L. Birnbaumer, and E. Stefani. Potentiation by the beta subunit of the ratio of the ionic current to the charge movement in the cardiac calcium channel. Science Wash. DC 262: 575-578, 1993.
15. Oberleithner, H., B. Schmidt, and P. Dietl. Fusion of epithelial cells: a model for studying cellular mechanisms of ion transport. Proc. Natl. Acad. Sci. USA 83: 3547-3551, 1986.
16. + channels. Physiol. Rev. 71: 1047-1083, 1991.
17. + channels. Neuron 11: 353-358, 1993.
18. Satin, J. C., J. W. Kyle, R. B. Rogart, H. A. Fozzard, and J. C. Makielski. Post-repolarization block of cloned sodium channels by saxitoxin: the contribution of pore-region amino acids. Biophys. J. 66: 1353-1363, 1994.
19. Sheets, M. F., and D. A. Hanck. Voltage-dependent open-state inactivation of cardiac sodium channels: gating currents studies with Anthopleurin-A toxin. J. Gen. Physiol. 106: 617-640, 1995.
20. Sheets, M. F., and R. E. TenEick. Whole-cell voltage clamp of cardiac sodium current. In: Ion Channels of Excitable Cells, edited by T. Narahashi. San Diego, CA: Academic, 1994, vol. 19, p. 169-188.
21. + channels expressed transiently in human embryonic kidney cells: biochemical and biophysical properties. Neuron 8: 663-676, 1992.
22. Zagotta, W. N., T. Hoshi, J. Dittman, and R. W. Aldrich. Shaker potassium channel gating. II. Transitions in the activation pathway. J. Gen. Physiol. 103: 279-319, 1994.