Comparison of methods for extracting DNA from formalin-fixed paraffin sections for nonisotopic PCR

Diagnostic Molecular Pathology

Volumn 5, Issue 3, 1996, Pages 220-224

  Frank, Thomas S ^a,b   Svoboda Newman, Suzette M ^a   Hsi, Eric D ^a  

^a UNIVERSITY OF MICHIGAN HOSPITALS   (United States)

^b Myriad Genetic Laboratories   (United States)

Author keywords

DNA Paraffin; Polymerase chain reaction

Indexed keywords

ARTICLE; DNA DETERMINATION; GENE AMPLIFICATION; HUMAN; LYMPH NODE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TISSUE FIXATION; TISSUE SECTION;

BASE SEQUENCE; DNA; FORMALDEHYDE; HUMANS; MOLECULAR SEQUENCE DATA; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; TISSUE FIXATION;

EID: 0029817020     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/00019606-199609000-00012     Document Type: Article

References (22)

1. Akane A, Matsubara K, Takahashi S, Kimura K. Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J Forensic Sci 1994;39:362-72.
2. An SF, Fleming KA. Removal of inhibitor(s) of the polymerase chain reaction from formalin fixed, paraffin wax embedded tissues. J Clin Pathol 1991;44:924-7.
3. Caduff RF, Johnston CM, Frank TS. Mutations of the Ki-ras oncogene in carcinoma of the endometrium. Am J Pathol 1995;146:182-8.
4. Chamberlain JS, Boehnke M, Frank TS. et al. BRCA1 maps proximal to D17S579 on chromosome 17q21 by genetic analysis. Am J Hum Genet 1993;52:792-8.
5. Cook SM, Bartos RE, Pierson CL, Frank TS. Detection and characterization of atypical mycobacteria by the polymerase chain reaction. Diagn Mol Pathol 1994;3:53-8.
6. Crisan D, Mattson JC. Retrospective DNA analysis using fixed tissue specimens [Review]. DNA Cell Biol. 1993;12: 455-64.
7. Dubeau L, Chandler LA, Gralow JR, Nichols PW, Jones PA. Southern blot analysis of DNA extracted from formalinfixed pathology specimens. Cancer Res 1986;46:2964-9.
8. Einsele H, Ehninger G, Steidle M, et al. Polymerase chain reaction to evaluate antiviral therapy for cytomegalovirus disease. Lancet 1991;338:1170-2.
9. Forsthoefel KF, Papp AC, Snyder PJ, Prior TW. Optimization of DNA extraction from formalin-fixed tissue and its clinical application in Duchenne muscular dystrophy. Am J Clin Pathol 1992;98:98-104.
10. Frank TS, Cook SM, Del Buono EA, Wilson MD. A simplified method for detecting cytomegalovirus by polymerase chain reaction from histologic sections of small biopsies. Mod Pathol 1992;5:449-54.
11. Goelz SE, Hamilton SR, Vogelstein B. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem Biophys Res Commun 1985;130:118-26.
12. Goldblum JR, Bartos RE, Carr KA, Frank TS. Hepatitis B and alterations of the p53 tumor suppressor gene in hepato-cellular carcinoma. Am J Surg Pathol 1993;17:1244-51.
13. Greer CE, Wheeler CM, Manos MM. Sample preparation and PCR amplification from paraffin-embedded tissues. PCR Methods Appl 1994;3:S113-22.
14. Heller MJ, Robinson RA, Burgart LJ, Teneyck CJ, Wilke WW, DNA extraction by sonication: a comparison of fresh, frozen, and paraffin-embedded tissues extracted for use in polymerase chain reaction assays. Mod Pathol 1992;5: 203-6.
15. Hsi ED, Greenson JK, Singleton TP, Siddiqui J, Schnitzer B, Ross CW. Detection of immunoglobulin heavy chain (IgH) gene rearrangement by polymerase chain reaction (PCR) in chronic active gastritis associated with Helicobacter pylori (CAG-Hp). Mod Pathol 1995;8:113A.
16. Impraim CC, Saiki RK, Erlich HA, Teplitz RL. Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides. Biochem Biophys Res Commun 1987;142:710-6.
17. Jackson DP, Lewis FA, Taylor GR, Boylston AW, Quirke P. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J Clin Pathol 1990;43:499-504.
18. Onadim Z, Cowell JK. Application of PCR amplification of DNA from paraffin embedded tissue sections to linkage analysis in familial retinoblastoma. J Med Genet 1991;28: 312-6.
19. Rogers BB, Alpert LC, Hine EA, Buffone GJ. Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction. Am J Pathol 1990;136:541-8.
20. Shibata D, Martin WJ, Arnheim N. Analysis of DNA sequences in forty-year-old paraffin-embedded thin-tissue sections: a bridge between molecular biology and classical histology. Cancer Res 1988;48:4564-6.
21. Shibata DK, Arnheim N, Martin WJ. Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. J Exp Med 1988;167:5225-30.
22. Smith M. Synthetic oligodeoxyribonucleotides as probes for nucleic acids and as primers in sequence determination. In: Weisman SM, ed. Methods of DNA and RNA sequencing. New York: Praeger, 1983:23-68.